Protocol for assessing regional pathology in the rodent optic nerve using longitudinal cryosections and cross-sectional electron microscopy
Summary: Visual system pathology has been established as a prominent feature in demyelinating diseases such as multiple sclerosis (MS). Here, we present a protocol for assessing regional pathology in the rodent optic nerve using longitudinal cryosections and cross-sectional electron microscopy. We d...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002667 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Summary: Visual system pathology has been established as a prominent feature in demyelinating diseases such as multiple sclerosis (MS). Here, we present a protocol for assessing regional pathology in the rodent optic nerve using longitudinal cryosections and cross-sectional electron microscopy. We describe steps for optic nerve dissection, post-fixation, myelin detection, slide scanning of myelin basic protein, and the analysis of demyelination. We then detail procedures for fluorescent antibody staining, analysis for transmission electron microscopy (TEM), and the analysis of myelinated and unmyelinated axons.For complete details on the use and execution of this protocol, please refer to Mey et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
|---|---|
| ISSN: | 2666-1667 |