Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions
Type 2 diabetes and obesity are increasing worldwide and linked to periodontitis, a chronic disease which is characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium. The mechanisms underlying the association of diabetes mellitus and obesity with periodont...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/796565 |
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| author | Marjan Nokhbehsaim Sema Keser Andressa Vilas Boas Nogueira Joni Augusto Cirelli Søren Jepsen Andreas Jäger Sigrun Eick James Deschner |
| author_facet | Marjan Nokhbehsaim Sema Keser Andressa Vilas Boas Nogueira Joni Augusto Cirelli Søren Jepsen Andreas Jäger Sigrun Eick James Deschner |
| author_sort | Marjan Nokhbehsaim |
| collection | DOAJ |
| description | Type 2 diabetes and obesity are increasing worldwide and linked to periodontitis, a chronic disease which is characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium. The mechanisms underlying the association of diabetes mellitus and obesity with periodontal destruction and compromised periodontal healing are not well understood, but decreased plasma levels of adiponectin, as found in diabetic and obese individuals, might be a critical mechanistic link. The aim of this in vitro study was to examine the effects of adiponectin on periodontal ligament (PDL) cells under normal and regenerative conditions, and to study the regulation of adiponectin and its receptors in these cells. Adiponectin stimulated significantly the expression of growth factors and extracellular matrix, proliferation, and in vitro wound healing, reduced significantly the constitutive tumor necrosis factor-α expression, and caused a significant upregulation of its own expression. The beneficial actions of enamel matrix derivative on a number of PDL cell functions critical for periodontal regeneration were partially enhanced by adiponectin. The periodontopathogen Porphyromonas gingivalis inhibited the adiponectin expression and stimulated the expression of its receptors. In conclusion, reduced levels of adiponectin, as found in type 2 diabetes and obesity, may compromise periodontal health and healing. |
| format | Article |
| id | doaj-art-33f46f7be12d4da9b621885a75ddb164 |
| institution | OA Journals |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-33f46f7be12d4da9b621885a75ddb1642025-08-20T02:24:15ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/796565796565Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative ConditionsMarjan Nokhbehsaim0Sema Keser1Andressa Vilas Boas Nogueira2Joni Augusto Cirelli3Søren Jepsen4Andreas Jäger5Sigrun Eick6James Deschner7Experimental Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry, UNESP, 14801-903 Araraquara, SP, BrazilClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, University of Bonn, 53111 Bonn, GermanyDepartment of Periodontology, Laboratory of Oral Microbiology, University of Bern, 3010 Bern, SwitzerlandExperimental Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyType 2 diabetes and obesity are increasing worldwide and linked to periodontitis, a chronic disease which is characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium. The mechanisms underlying the association of diabetes mellitus and obesity with periodontal destruction and compromised periodontal healing are not well understood, but decreased plasma levels of adiponectin, as found in diabetic and obese individuals, might be a critical mechanistic link. The aim of this in vitro study was to examine the effects of adiponectin on periodontal ligament (PDL) cells under normal and regenerative conditions, and to study the regulation of adiponectin and its receptors in these cells. Adiponectin stimulated significantly the expression of growth factors and extracellular matrix, proliferation, and in vitro wound healing, reduced significantly the constitutive tumor necrosis factor-α expression, and caused a significant upregulation of its own expression. The beneficial actions of enamel matrix derivative on a number of PDL cell functions critical for periodontal regeneration were partially enhanced by adiponectin. The periodontopathogen Porphyromonas gingivalis inhibited the adiponectin expression and stimulated the expression of its receptors. In conclusion, reduced levels of adiponectin, as found in type 2 diabetes and obesity, may compromise periodontal health and healing.http://dx.doi.org/10.1155/2014/796565 |
| spellingShingle | Marjan Nokhbehsaim Sema Keser Andressa Vilas Boas Nogueira Joni Augusto Cirelli Søren Jepsen Andreas Jäger Sigrun Eick James Deschner Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions Journal of Diabetes Research |
| title | Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions |
| title_full | Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions |
| title_fullStr | Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions |
| title_full_unstemmed | Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions |
| title_short | Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions |
| title_sort | beneficial effects of adiponectin on periodontal ligament cells under normal and regenerative conditions |
| url | http://dx.doi.org/10.1155/2014/796565 |
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