Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway
Abstract Background Diabetes-associated cognitive dysfunction has become a major public health concern. However, the mechanisms driving this disease are elusive. Herein, we explored how electroacupuncture improves learning and memory function in diabetic rats. Methods The diabetic model was establis...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
|
| Series: | Journal of Physiological Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12576-022-00854-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850157112932433920 |
|---|---|
| author | Xia Ge Ling Wang Qianqian Cui Hongli Yan Zhongbao Wang Shandong Ye Qingping Zhang Aihua Fei |
| author_facet | Xia Ge Ling Wang Qianqian Cui Hongli Yan Zhongbao Wang Shandong Ye Qingping Zhang Aihua Fei |
| author_sort | Xia Ge |
| collection | DOAJ |
| description | Abstract Background Diabetes-associated cognitive dysfunction has become a major public health concern. However, the mechanisms driving this disease are elusive. Herein, we explored how electroacupuncture improves learning and memory function in diabetic rats. Methods The diabetic model was established by intraperitoneal injection of streptozotocin (STZ) in adult Sprague–Dawley rats. Rats were fed on high-fat and high-sugar diets. Learning and memory functions were assessed using behavioral tests. The hematoxylin and eosin (H&E) staining, Western blotting, real-time PCR, ELISA, immunohistochemistry, and transmission electronic microscopy (TEM) was performed to test related indicators. Results High-fat and high-sugar diets impaired learning and memory function in rats, while electroacupuncture treatment reversed these changes. The model group presented highly prolonged escape latency compared to the control group, indicating impaired learning and memory functions. The TEM examination showed that electroacupuncture enhanced Aβ clearance and mitochondrial autophagy in hippocampal neuronal cells by increasing DISC1 expression. Conclusions Electroacupuncture improves learning and memory function in diabetic rats by increasing DISC1 expression to promote mitophagy. This enhanced Aβ clearance, alleviating cytotoxicity in hippocampal neuronal cells. |
| format | Article |
| id | doaj-art-33e07e55e4154d159c3f696f7a1f9c93 |
| institution | OA Journals |
| issn | 1880-6546 1880-6562 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Physiological Sciences |
| spelling | doaj-art-33e07e55e4154d159c3f696f7a1f9c932025-08-20T02:24:17ZengElsevierJournal of Physiological Sciences1880-65461880-65622022-11-0172111210.1186/s12576-022-00854-0Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathwayXia Ge0Ling Wang1Qianqian Cui2Hongli Yan3Zhongbao Wang4Shandong Ye5Qingping Zhang6Aihua Fei7Department of Endocrinology, The Second Affiliated Hospital of Anhui University of Chinese MedicineCollege of Second Clinical Medical, Anhui University of Chinese MedicineDepartment of Emergency, The Second Affiliated Hospital of Anhui University of Chinese MedicineDepartment of Acupuncture-Moxibustion and Rehabilitation, Mingguang Hospital of Traditional Chinese MedicineCollege of Third Clinical Medical, Anhui University of Chinese MedicineDepartment of Endocrinology, The First Affiliated Hospital of University of Science and Technology of ChinaCollege of Acupuncture-Moxibustion and Tuina, Anhui University of Chinese MedicineDepartment of Endocrinology, The Second Affiliated Hospital of Anhui University of Chinese MedicineAbstract Background Diabetes-associated cognitive dysfunction has become a major public health concern. However, the mechanisms driving this disease are elusive. Herein, we explored how electroacupuncture improves learning and memory function in diabetic rats. Methods The diabetic model was established by intraperitoneal injection of streptozotocin (STZ) in adult Sprague–Dawley rats. Rats were fed on high-fat and high-sugar diets. Learning and memory functions were assessed using behavioral tests. The hematoxylin and eosin (H&E) staining, Western blotting, real-time PCR, ELISA, immunohistochemistry, and transmission electronic microscopy (TEM) was performed to test related indicators. Results High-fat and high-sugar diets impaired learning and memory function in rats, while electroacupuncture treatment reversed these changes. The model group presented highly prolonged escape latency compared to the control group, indicating impaired learning and memory functions. The TEM examination showed that electroacupuncture enhanced Aβ clearance and mitochondrial autophagy in hippocampal neuronal cells by increasing DISC1 expression. Conclusions Electroacupuncture improves learning and memory function in diabetic rats by increasing DISC1 expression to promote mitophagy. This enhanced Aβ clearance, alleviating cytotoxicity in hippocampal neuronal cells.https://doi.org/10.1186/s12576-022-00854-0Diabetes-associated cognitive impairmentElectroacupunctureMitophagyDISC1 |
| spellingShingle | Xia Ge Ling Wang Qianqian Cui Hongli Yan Zhongbao Wang Shandong Ye Qingping Zhang Aihua Fei Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway Journal of Physiological Sciences Diabetes-associated cognitive impairment Electroacupuncture Mitophagy DISC1 |
| title | Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway |
| title_full | Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway |
| title_fullStr | Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway |
| title_full_unstemmed | Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway |
| title_short | Electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway |
| title_sort | electroacupuncture improves cognitive impairment in diabetic cognitive dysfunction rats by regulating the mitochondrial autophagy pathway |
| topic | Diabetes-associated cognitive impairment Electroacupuncture Mitophagy DISC1 |
| url | https://doi.org/10.1186/s12576-022-00854-0 |
| work_keys_str_mv | AT xiage electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT lingwang electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT qianqiancui electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT hongliyan electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT zhongbaowang electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT shandongye electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT qingpingzhang electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway AT aihuafei electroacupunctureimprovescognitiveimpairmentindiabeticcognitivedysfunctionratsbyregulatingthemitochondrialautophagypathway |