Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is a major challenge for food safety and public health. This study aimed to investigate the potential role of oysters sold in Egypt as a source for Staphylococcus aureus (S. aureus), MRSA, and multidrug-resistant methicillin-resistant Staph...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-88743-5 |
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| author | Rahma Mohammed Sara M. Nader Dalia A. Hamza Maha A. Sabry |
| author_facet | Rahma Mohammed Sara M. Nader Dalia A. Hamza Maha A. Sabry |
| author_sort | Rahma Mohammed |
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| description | Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is a major challenge for food safety and public health. This study aimed to investigate the potential role of oysters sold in Egypt as a source for Staphylococcus aureus (S. aureus), MRSA, and multidrug-resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA). It also examined the occurrence of tsst-1 virulence gene and assessed the associated zoonotic risks. Thirty-three pooled fresh oyster samples were acquired from different retail fish markets in Egypt. S. aureus was identified by conventional culture-based and molecular methods. Antimicrobial resistance was performed by the disk-diffusion method, and the multiple antibiotic resistance index (MARI) was calculated. Antimicrobial resistance (mecA and mecC) and virulence (tsst-1) genes were screened using polymerase chain reaction. The clustering of virulent MDR-MRSA isolates was performed using R with the pheatmap package. The prevalence of S. aureus was 39.4% (13 /33), and 77% of them (10/13) were classified as MDR with MARI values greater than 0.2. Notably, 46.2% (6 /13) of isolates were identified as MRSA and all MRSA isolates displayed MDR. Of the MDR-MRSA isolates, 66.7% (4 /6) possessed the mecA gene, while 16.7% (1 /6) tested positive for the mecC gene. Additionally, the tsst-1 gene was identified in one isolate (16.7%). Interestingly, two MDR-MRSA isolates exhibited a clustered pattern. The study sheds light on the emergence of virulent MDR-MRSA isolates in Egyptian oysters. It highlights oysters as a potential source for spreading these isolates within aquatic ecosystems, posing a threat to food safety and public health. |
| format | Article |
| id | doaj-art-33decd085d114901996b1cc9cf1184b9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-33decd085d114901996b1cc9cf1184b92025-02-09T12:37:44ZengNature PortfolioScientific Reports2045-23222025-02-011511910.1038/s41598-025-88743-5Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oystersRahma Mohammed0Sara M. Nader1Dalia A. Hamza2Maha A. Sabry3Department of Zoonoses, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Zoonoses, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Zoonoses, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Zoonoses, Faculty of Veterinary Medicine, Cairo UniversityAbstract Methicillin-resistant Staphylococcus aureus (MRSA) is a major challenge for food safety and public health. This study aimed to investigate the potential role of oysters sold in Egypt as a source for Staphylococcus aureus (S. aureus), MRSA, and multidrug-resistant methicillin-resistant Staphylococcus aureus (MDR-MRSA). It also examined the occurrence of tsst-1 virulence gene and assessed the associated zoonotic risks. Thirty-three pooled fresh oyster samples were acquired from different retail fish markets in Egypt. S. aureus was identified by conventional culture-based and molecular methods. Antimicrobial resistance was performed by the disk-diffusion method, and the multiple antibiotic resistance index (MARI) was calculated. Antimicrobial resistance (mecA and mecC) and virulence (tsst-1) genes were screened using polymerase chain reaction. The clustering of virulent MDR-MRSA isolates was performed using R with the pheatmap package. The prevalence of S. aureus was 39.4% (13 /33), and 77% of them (10/13) were classified as MDR with MARI values greater than 0.2. Notably, 46.2% (6 /13) of isolates were identified as MRSA and all MRSA isolates displayed MDR. Of the MDR-MRSA isolates, 66.7% (4 /6) possessed the mecA gene, while 16.7% (1 /6) tested positive for the mecC gene. Additionally, the tsst-1 gene was identified in one isolate (16.7%). Interestingly, two MDR-MRSA isolates exhibited a clustered pattern. The study sheds light on the emergence of virulent MDR-MRSA isolates in Egyptian oysters. It highlights oysters as a potential source for spreading these isolates within aquatic ecosystems, posing a threat to food safety and public health.https://doi.org/10.1038/s41598-025-88743-5S. aureusMRSAMulti-drug resistanceVirulence factorsOystersEgypt |
| spellingShingle | Rahma Mohammed Sara M. Nader Dalia A. Hamza Maha A. Sabry Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters Scientific Reports S. aureus MRSA Multi-drug resistance Virulence factors Oysters Egypt |
| title | Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters |
| title_full | Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters |
| title_fullStr | Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters |
| title_full_unstemmed | Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters |
| title_short | Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters |
| title_sort | public health implications of multidrugresistant and methicillinresistant staphylococcus aureus in retail oysters |
| topic | S. aureus MRSA Multi-drug resistance Virulence factors Oysters Egypt |
| url | https://doi.org/10.1038/s41598-025-88743-5 |
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