Mechanism of NOTCH2 in promoting intrahepatic bile duct development
Abstract Background The Notch signaling pathway plays a crucial role in intrahepatic bile duct development. Here, we aimed to investigate the effect of the Notch receptor, NOTCH2, on intrahepatic bile duct development to better understand congenital intrahepatic bile duct dysplasia. Results Estradio...
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| Format: | Article |
| Language: | English |
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BMC
2025-08-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-025-04217-y |
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| author | Chen Dong Sheng-xuan Liu Biao Zou Sai-nan Shu Ben-ping Zhang |
| author_facet | Chen Dong Sheng-xuan Liu Biao Zou Sai-nan Shu Ben-ping Zhang |
| author_sort | Chen Dong |
| collection | DOAJ |
| description | Abstract Background The Notch signaling pathway plays a crucial role in intrahepatic bile duct development. Here, we aimed to investigate the effect of the Notch receptor, NOTCH2, on intrahepatic bile duct development to better understand congenital intrahepatic bile duct dysplasia. Results Estradiol increased NOTCH2 and its downstream proteins expression, which promoted the differentiation of hepatoblasts into intrahepatic cholangiocytes and the development of intrahepatic bile ducts by upregulating the Notch signaling pathway. NOTCH2 siRNA inhibited the above processes (P < 0.05). There was no significant difference between estradiol and estradiol + non-targeting siRNA groups (P > 0.1). Conclusions In conclusion, the activation of the Notch signaling pathway leads to increased NOTCH2 expression, which promotes the differentiation of hepatoblasts into intrahepatic cholangiocytes and the development of intrahepatic bile ducts during embryonic stages in C57BL/6CrSlc mice. The results of this study may provide a theoretical basis for infantile intrahepatic cholestasis treatment. |
| format | Article |
| id | doaj-art-33caf834cf354c16bb9ca52fef84bc99 |
| institution | Kabale University |
| issn | 1471-230X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj-art-33caf834cf354c16bb9ca52fef84bc992025-08-24T11:33:02ZengBMCBMC Gastroenterology1471-230X2025-08-012511910.1186/s12876-025-04217-yMechanism of NOTCH2 in promoting intrahepatic bile duct developmentChen Dong0Sheng-xuan Liu1Biao Zou2Sai-nan Shu3Ben-ping Zhang4Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Endocrinology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Background The Notch signaling pathway plays a crucial role in intrahepatic bile duct development. Here, we aimed to investigate the effect of the Notch receptor, NOTCH2, on intrahepatic bile duct development to better understand congenital intrahepatic bile duct dysplasia. Results Estradiol increased NOTCH2 and its downstream proteins expression, which promoted the differentiation of hepatoblasts into intrahepatic cholangiocytes and the development of intrahepatic bile ducts by upregulating the Notch signaling pathway. NOTCH2 siRNA inhibited the above processes (P < 0.05). There was no significant difference between estradiol and estradiol + non-targeting siRNA groups (P > 0.1). Conclusions In conclusion, the activation of the Notch signaling pathway leads to increased NOTCH2 expression, which promotes the differentiation of hepatoblasts into intrahepatic cholangiocytes and the development of intrahepatic bile ducts during embryonic stages in C57BL/6CrSlc mice. The results of this study may provide a theoretical basis for infantile intrahepatic cholestasis treatment.https://doi.org/10.1186/s12876-025-04217-yNOTCH2Notch signaling pathwayHepatoblastIntrahepatic bile duct |
| spellingShingle | Chen Dong Sheng-xuan Liu Biao Zou Sai-nan Shu Ben-ping Zhang Mechanism of NOTCH2 in promoting intrahepatic bile duct development BMC Gastroenterology NOTCH2 Notch signaling pathway Hepatoblast Intrahepatic bile duct |
| title | Mechanism of NOTCH2 in promoting intrahepatic bile duct development |
| title_full | Mechanism of NOTCH2 in promoting intrahepatic bile duct development |
| title_fullStr | Mechanism of NOTCH2 in promoting intrahepatic bile duct development |
| title_full_unstemmed | Mechanism of NOTCH2 in promoting intrahepatic bile duct development |
| title_short | Mechanism of NOTCH2 in promoting intrahepatic bile duct development |
| title_sort | mechanism of notch2 in promoting intrahepatic bile duct development |
| topic | NOTCH2 Notch signaling pathway Hepatoblast Intrahepatic bile duct |
| url | https://doi.org/10.1186/s12876-025-04217-y |
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