Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip
Cell fusion is a widely employed process in various biological procedures, demonstrating significant application value in biotechnology. Cell pairing is a crucial manipulation for cell fusion. Standard fusion techniques, however, often provide poor and random cell contact, leading to low yields. In...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2025-03-01
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| Series: | APL Bioengineering |
| Online Access: | http://dx.doi.org/10.1063/5.0250472 |
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| author | Xuefeng Wang Yaqi Bai Xiaoling Zhang Wei Li Jun Yang Ning Hu |
| author_facet | Xuefeng Wang Yaqi Bai Xiaoling Zhang Wei Li Jun Yang Ning Hu |
| author_sort | Xuefeng Wang |
| collection | DOAJ |
| description | Cell fusion is a widely employed process in various biological procedures, demonstrating significant application value in biotechnology. Cell pairing is a crucial manipulation for cell fusion. Standard fusion techniques, however, often provide poor and random cell contact, leading to low yields. In this study, we present a novel microfluidic device that utilizes a three-path symmetrical channel hydrodynamic capture method to achieve high-efficiency cell capture and pairing. The device contains several symmetrical channels and capture units, enabling three-path capture of two kinds of cells. To better understand the conditions necessary for effective cell pairing, we established a theoretical model of the three-path trapping flow field and conducted a qualitative force analysis on cells. Using K562 cells to explore the effect of different volumetric flow ratios of symmetric channels on cell capture and pairing efficiency, we finally got the optimized structure and obtained a single-cell capture efficiency of approximately 95.6 ± 2.0% and a cell pairing efficiency of approximately 83.3 ± 8.8%. Subsequently, electrofusion experiments were carried out on the paired cells, resulting in a fusion efficiency of approximately 77.8 ± 9.6%. |
| format | Article |
| id | doaj-art-33ba686f91ae41cabf7c9f4086d9b2dd |
| institution | OA Journals |
| issn | 2473-2877 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Bioengineering |
| spelling | doaj-art-33ba686f91ae41cabf7c9f4086d9b2dd2025-08-20T01:55:52ZengAIP Publishing LLCAPL Bioengineering2473-28772025-03-0191016111016111-1110.1063/5.0250472Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chipXuefeng Wang0Yaqi Bai1Xiaoling Zhang2Wei Li3Jun Yang4Ning Hu5Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400044, ChinaSchool of Smart Health, Chongqing College of Electronic Engineering, Chongqing 401331, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400044, ChinaKey Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400044, ChinaCell fusion is a widely employed process in various biological procedures, demonstrating significant application value in biotechnology. Cell pairing is a crucial manipulation for cell fusion. Standard fusion techniques, however, often provide poor and random cell contact, leading to low yields. In this study, we present a novel microfluidic device that utilizes a three-path symmetrical channel hydrodynamic capture method to achieve high-efficiency cell capture and pairing. The device contains several symmetrical channels and capture units, enabling three-path capture of two kinds of cells. To better understand the conditions necessary for effective cell pairing, we established a theoretical model of the three-path trapping flow field and conducted a qualitative force analysis on cells. Using K562 cells to explore the effect of different volumetric flow ratios of symmetric channels on cell capture and pairing efficiency, we finally got the optimized structure and obtained a single-cell capture efficiency of approximately 95.6 ± 2.0% and a cell pairing efficiency of approximately 83.3 ± 8.8%. Subsequently, electrofusion experiments were carried out on the paired cells, resulting in a fusion efficiency of approximately 77.8 ± 9.6%.http://dx.doi.org/10.1063/5.0250472 |
| spellingShingle | Xuefeng Wang Yaqi Bai Xiaoling Zhang Wei Li Jun Yang Ning Hu Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip APL Bioengineering |
| title | Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip |
| title_full | Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip |
| title_fullStr | Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip |
| title_full_unstemmed | Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip |
| title_short | Hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip |
| title_sort | hydrodynamic efficient cell capture and pairing method on microfluidic cell electrofusion chip |
| url | http://dx.doi.org/10.1063/5.0250472 |
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