Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes
Abstract Eucommia ulmoides (E. ulmoides) is a valuable gum-producing plant and traditional Chinese medicine. The utilization value of E. ulmoides varies according to the sex of the plant, and due to its perennial characteristics, the identification of male and female is challenging. To meet producti...
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Nature Portfolio
2024-12-01
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| author | Jinyu Yang Runying Wang Na Ren De-gang Zhao Xiaozhen Huang Yichen Zhao |
| author_facet | Jinyu Yang Runying Wang Na Ren De-gang Zhao Xiaozhen Huang Yichen Zhao |
| author_sort | Jinyu Yang |
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| description | Abstract Eucommia ulmoides (E. ulmoides) is a valuable gum-producing plant and traditional Chinese medicine. The utilization value of E. ulmoides varies according to the sex of the plant, and due to its perennial characteristics, the identification of male and female is challenging. To meet production demands, gender selection through grafting has been employed. The fusion of rootstock and scion cells in grafted plants can be enhanced by β-1, 4-glucanase, thus improving the grafting survival rate. However, extracting β-1, 4-glucanase in vivo poses difficulties. In this study, the β-1, 4-glucanase gene of E. ulmoides was cloned, and the total length of the gene was 1917 bp, encoding 638 amino acids. Pichia pastoris engineering bacteria were used to express β-1, 4-glucanase. The optimal fermentation conditions included a pH of 6, a temperature of 28 ℃, a methanol content of 1.5%, and a fermentation period of 96 hours. After purification, the enzyme activity of the target protein was measured to be 286.35 U/mL. Protein concentrations of 5 mg/mL, 10 mg/mL, and 15 mg/mL were tested for E. ulmoides grafting. The results showed that the protein could promote wound healing and improve the survival rate of E. ulmoides grafting. In conclusion, this study successfully developed an enzyme that improves the survival rate of E. ulmoides grafting, and provides valuable insights for the breeding of male and female E. ulmoides grafts. |
| format | Article |
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| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-33b89b4e4a834e40b0ab3419a901c52b2025-08-20T02:31:04ZengNature PortfolioScientific Reports2045-23222024-12-0114111010.1038/s41598-024-81695-2Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genesJinyu Yang0Runying Wang1Na Ren2De-gang Zhao3Xiaozhen Huang4Yichen Zhao5College of Life Sciences, College of Tea Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Life Sciences, College of Tea Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Life Sciences, College of Tea Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Life Sciences, College of Tea Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Life Sciences, College of Tea Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityCollege of Life Sciences, College of Tea Sciences, The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou UniversityAbstract Eucommia ulmoides (E. ulmoides) is a valuable gum-producing plant and traditional Chinese medicine. The utilization value of E. ulmoides varies according to the sex of the plant, and due to its perennial characteristics, the identification of male and female is challenging. To meet production demands, gender selection through grafting has been employed. The fusion of rootstock and scion cells in grafted plants can be enhanced by β-1, 4-glucanase, thus improving the grafting survival rate. However, extracting β-1, 4-glucanase in vivo poses difficulties. In this study, the β-1, 4-glucanase gene of E. ulmoides was cloned, and the total length of the gene was 1917 bp, encoding 638 amino acids. Pichia pastoris engineering bacteria were used to express β-1, 4-glucanase. The optimal fermentation conditions included a pH of 6, a temperature of 28 ℃, a methanol content of 1.5%, and a fermentation period of 96 hours. After purification, the enzyme activity of the target protein was measured to be 286.35 U/mL. Protein concentrations of 5 mg/mL, 10 mg/mL, and 15 mg/mL were tested for E. ulmoides grafting. The results showed that the protein could promote wound healing and improve the survival rate of E. ulmoides grafting. In conclusion, this study successfully developed an enzyme that improves the survival rate of E. ulmoides grafting, and provides valuable insights for the breeding of male and female E. ulmoides grafts.https://doi.org/10.1038/s41598-024-81695-2DioecismProtein expressionPichia pastorisGrafting |
| spellingShingle | Jinyu Yang Runying Wang Na Ren De-gang Zhao Xiaozhen Huang Yichen Zhao Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes Scientific Reports Dioecism Protein expression Pichia pastoris Grafting |
| title | Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes |
| title_full | Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes |
| title_fullStr | Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes |
| title_full_unstemmed | Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes |
| title_short | Exogenous application of Eucommia ulmoides β-1, 4-glucanase promotes propagation by increasing the expression of wound healing genes |
| title_sort | exogenous application of eucommia ulmoides β 1 4 glucanase promotes propagation by increasing the expression of wound healing genes |
| topic | Dioecism Protein expression Pichia pastoris Grafting |
| url | https://doi.org/10.1038/s41598-024-81695-2 |
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