Effects of antibiotics on callus induction in sorghum and subsequent growth of callus
Growth inhibition of Agrobacterium by 3 different antibiotics was demonstrated. Carbenicillin and cefotaxime gave better results than ampicillin in inhibiting Agrobacterium growth. Cefotaxime was significantly more toxic than carbenicillin in induction of callus in sorghum. Carbenicillin of concentr...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2004-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0221 |
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| author | ZHANG Ming-zhou CUI Hai-rui SHU Qing-yao XIA Yin-wu |
| author_facet | ZHANG Ming-zhou CUI Hai-rui SHU Qing-yao XIA Yin-wu |
| author_sort | ZHANG Ming-zhou |
| collection | DOAJ |
| description | Growth inhibition of Agrobacterium by 3 different antibiotics was demonstrated. Carbenicillin and cefotaxime gave better results than ampicillin in inhibiting Agrobacterium growth. Cefotaxime was significantly more toxic than carbenicillin in induction of callus in sorghum. Carbenicillin of concentration below 500 mg/L in MS medium showed positive effects on sorghum callus growth. The regeneration rate of shoots from sorghum callus, however, was decreased with increase of carbenicillin level from 50 mg/L to 1000 mg/L in MS medium. The ideal concentration of carbenicillin was 250 mg/L when used in genetic transformation of sorghum mediated by Agrobacterium. Sorghum callus was more sensitive to hygromycin than to kanamycin. Considering the rate of browning (dying), the responses to kanamycin and hygromycin were different among different sorghum genotypes. With hygromycin as the selection marker, the optimal selecting pressure was 50-75 mg/L. |
| format | Article |
| id | doaj-art-33ac571b3d2f48a1983723eb0bcd1d91 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2004-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-33ac571b3d2f48a1983723eb0bcd1d912025-08-20T03:34:08ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552004-03-013022122510.3785/1008-9209.2004.02.022110089209Effects of antibiotics on callus induction in sorghum and subsequent growth of callusZHANG Ming-zhouCUI Hai-ruiSHU Qing-yaoXIA Yin-wuGrowth inhibition of Agrobacterium by 3 different antibiotics was demonstrated. Carbenicillin and cefotaxime gave better results than ampicillin in inhibiting Agrobacterium growth. Cefotaxime was significantly more toxic than carbenicillin in induction of callus in sorghum. Carbenicillin of concentration below 500 mg/L in MS medium showed positive effects on sorghum callus growth. The regeneration rate of shoots from sorghum callus, however, was decreased with increase of carbenicillin level from 50 mg/L to 1000 mg/L in MS medium. The ideal concentration of carbenicillin was 250 mg/L when used in genetic transformation of sorghum mediated by Agrobacterium. Sorghum callus was more sensitive to hygromycin than to kanamycin. Considering the rate of browning (dying), the responses to kanamycin and hygromycin were different among different sorghum genotypes. With hygromycin as the selection marker, the optimal selecting pressure was 50-75 mg/L.https://www.academax.com/doi/10.3785/1008-9209.2004.02.0221sorghum<italic>Agrobacterium</italic>antibioticscallus |
| spellingShingle | ZHANG Ming-zhou CUI Hai-rui SHU Qing-yao XIA Yin-wu Effects of antibiotics on callus induction in sorghum and subsequent growth of callus 浙江大学学报. 农业与生命科学版 sorghum <italic>Agrobacterium</italic> antibiotics callus |
| title | Effects of antibiotics on callus induction in sorghum and subsequent growth of callus |
| title_full | Effects of antibiotics on callus induction in sorghum and subsequent growth of callus |
| title_fullStr | Effects of antibiotics on callus induction in sorghum and subsequent growth of callus |
| title_full_unstemmed | Effects of antibiotics on callus induction in sorghum and subsequent growth of callus |
| title_short | Effects of antibiotics on callus induction in sorghum and subsequent growth of callus |
| title_sort | effects of antibiotics on callus induction in sorghum and subsequent growth of callus |
| topic | sorghum <italic>Agrobacterium</italic> antibiotics callus |
| url | https://www.academax.com/doi/10.3785/1008-9209.2004.02.0221 |
| work_keys_str_mv | AT zhangmingzhou effectsofantibioticsoncallusinductioninsorghumandsubsequentgrowthofcallus AT cuihairui effectsofantibioticsoncallusinductioninsorghumandsubsequentgrowthofcallus AT shuqingyao effectsofantibioticsoncallusinductioninsorghumandsubsequentgrowthofcallus AT xiayinwu effectsofantibioticsoncallusinductioninsorghumandsubsequentgrowthofcallus |