SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis
Abstract Mapping the spatial organization of tissues is critical to understanding organ biology in health and disease. Developments in multiplexed antibody-based, fluorescence labelling methods have provided unique insights into tissue microenvironments. However, many current methods have a variety...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-04-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-08052-5 |
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| author | Michael D. Anderson Abigail Plone Jeffrey La Madison Wong Krishnan Raghunathan Jocelyn A. Silvester Jay R. Thiagarajah |
| author_facet | Michael D. Anderson Abigail Plone Jeffrey La Madison Wong Krishnan Raghunathan Jocelyn A. Silvester Jay R. Thiagarajah |
| author_sort | Michael D. Anderson |
| collection | DOAJ |
| description | Abstract Mapping the spatial organization of tissues is critical to understanding organ biology in health and disease. Developments in multiplexed antibody-based, fluorescence labelling methods have provided unique insights into tissue microenvironments. However, many current methods have a variety of limitations that reduce their practical utilization including cost, time and technical complexity. To address these drawbacks, we developed Spatial Photo-inactivation Enhanced Cyclic Target REsolved multiPlexing (SPECTRE-Plex). SPECTRE-Plex is a relatively low cost, end-to-end technique based on a series of methods that significantly improve speed, automation, and resolution of cyclic multiplex immunofluorescence imaging. We describe a representative example of the application of the method by investigating spatial cellular and neighborhood changes in the proximal small intestine between healthy tissue and active celiac disease. |
| format | Article |
| id | doaj-art-33aa737f78ab4f8ba7a1df7e07059593 |
| institution | OA Journals |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-33aa737f78ab4f8ba7a1df7e070595932025-08-20T02:17:56ZengNature PortfolioCommunications Biology2399-36422025-04-01811610.1038/s42003-025-08052-5SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysisMichael D. Anderson0Abigail Plone1Jeffrey La2Madison Wong3Krishnan Raghunathan4Jocelyn A. Silvester5Jay R. Thiagarajah6Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolDivision of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolDivision of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolDivision of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolDivision of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolDivision of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolDivision of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Harvard Medical SchoolAbstract Mapping the spatial organization of tissues is critical to understanding organ biology in health and disease. Developments in multiplexed antibody-based, fluorescence labelling methods have provided unique insights into tissue microenvironments. However, many current methods have a variety of limitations that reduce their practical utilization including cost, time and technical complexity. To address these drawbacks, we developed Spatial Photo-inactivation Enhanced Cyclic Target REsolved multiPlexing (SPECTRE-Plex). SPECTRE-Plex is a relatively low cost, end-to-end technique based on a series of methods that significantly improve speed, automation, and resolution of cyclic multiplex immunofluorescence imaging. We describe a representative example of the application of the method by investigating spatial cellular and neighborhood changes in the proximal small intestine between healthy tissue and active celiac disease.https://doi.org/10.1038/s42003-025-08052-5 |
| spellingShingle | Michael D. Anderson Abigail Plone Jeffrey La Madison Wong Krishnan Raghunathan Jocelyn A. Silvester Jay R. Thiagarajah SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis Communications Biology |
| title | SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis |
| title_full | SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis |
| title_fullStr | SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis |
| title_full_unstemmed | SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis |
| title_short | SPECTREPlex: an automated, fast, high-resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis |
| title_sort | spectreplex an automated fast high resolution enabled approach for multiplexed cyclic imaging and tissue spatial analysis |
| url | https://doi.org/10.1038/s42003-025-08052-5 |
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