Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm

Environmental contamination with Klebsiella pneumoniae biofilm can be a source of healthcare-associated infections. Disinfection with various biocidal active substances is usually the method of choice to remove contamination with biofilm. In this study we tested 13 different disinfection protocols u...

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Main Authors: Piletić Kaća, Mežnarić Silvestar, Keržić Eli, Oder Martina, Gobin Ivana
Format: Article
Language:English
Published: Sciendo 2024-12-01
Series:Arhiv za Higijenu Rada i Toksikologiju
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Online Access:https://doi.org/10.2478/aiht-2024-75-3920
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author Piletić Kaća
Mežnarić Silvestar
Keržić Eli
Oder Martina
Gobin Ivana
author_facet Piletić Kaća
Mežnarić Silvestar
Keržić Eli
Oder Martina
Gobin Ivana
author_sort Piletić Kaća
collection DOAJ
description Environmental contamination with Klebsiella pneumoniae biofilm can be a source of healthcare-associated infections. Disinfection with various biocidal active substances is usually the method of choice to remove contamination with biofilm. In this study we tested 13 different disinfection protocols using gaseous ozone, citric acid, and three working concentrations of benzalkonium chloride-based professional disinfecting products on 24-hour-old biofilms formed by two K. pneumoniae strains on ceramic tiles. All tested protocols significantly reduced total bacterial counts compared to control, varying from a log10 CFU reduction factor of 1.4 to 5.6. Disinfection combining two or more biocidal active substances resulted in significantly better anti-biofilm efficacy than disinfection with single substances, and the most effective combination for both strains was that of citric acid, gaseous ozone, and benzalkonium chloride. This follow up study is limited to K. pneumoniae alone, and to overcome this limitation, future studies should include more bacterial species, both Gram-positive and Gramnegative, and more samples for us to find optimal disinfection protocols, applicable in real hospital settings.
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series Arhiv za Higijenu Rada i Toksikologiju
spelling doaj-art-33a2b0bfacc64dfc9ffeb611d6dac4b12025-08-20T02:17:47ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122024-12-0175428929610.2478/aiht-2024-75-3920Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilmPiletić Kaća0Mežnarić Silvestar1Keržić Eli2Oder Martina3Gobin Ivana41University of Rijeka Faculty of Medicine, Department of Microbiology and Parasitology, Rijeka, Croatia2University of Rijeka Faculty of Medicine, Department of Basic and Clinical Pharmacology and Toxicology, Rijeka, Croatia3University of Ljubljana Biotechnical Faculty, Department of Wood Science and Technology, Ljubljana, Slovenia4University of Ljubljana Faculty of Health Sciences, Department of Sanitary Engineering, Ljubljana, Slovenia1University of Rijeka Faculty of Medicine, Department of Microbiology and Parasitology, Rijeka, CroatiaEnvironmental contamination with Klebsiella pneumoniae biofilm can be a source of healthcare-associated infections. Disinfection with various biocidal active substances is usually the method of choice to remove contamination with biofilm. In this study we tested 13 different disinfection protocols using gaseous ozone, citric acid, and three working concentrations of benzalkonium chloride-based professional disinfecting products on 24-hour-old biofilms formed by two K. pneumoniae strains on ceramic tiles. All tested protocols significantly reduced total bacterial counts compared to control, varying from a log10 CFU reduction factor of 1.4 to 5.6. Disinfection combining two or more biocidal active substances resulted in significantly better anti-biofilm efficacy than disinfection with single substances, and the most effective combination for both strains was that of citric acid, gaseous ozone, and benzalkonium chloride. This follow up study is limited to K. pneumoniae alone, and to overcome this limitation, future studies should include more bacterial species, both Gram-positive and Gramnegative, and more samples for us to find optimal disinfection protocols, applicable in real hospital settings.https://doi.org/10.2478/aiht-2024-75-3920benzalkonium chloridecitric acidk. pneumoniaeozonebenzaklonijev kloridbiofilmk. pneumoniaelimunska kiselinaozon
spellingShingle Piletić Kaća
Mežnarić Silvestar
Keržić Eli
Oder Martina
Gobin Ivana
Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm
Arhiv za Higijenu Rada i Toksikologiju
benzalkonium chloride
citric acid
k. pneumoniae
ozone
benzaklonijev klorid
biofilm
k. pneumoniae
limunska kiselina
ozon
title Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm
title_full Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm
title_fullStr Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm
title_full_unstemmed Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm
title_short Comparison of different disinfection protocols against contamination of ceramic surfaces with Klebsiella pneumoniae biofilm
title_sort comparison of different disinfection protocols against contamination of ceramic surfaces with klebsiella pneumoniae biofilm
topic benzalkonium chloride
citric acid
k. pneumoniae
ozone
benzaklonijev klorid
biofilm
k. pneumoniae
limunska kiselina
ozon
url https://doi.org/10.2478/aiht-2024-75-3920
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