Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum
Summary: The Planctomycetota-Chlamydiota-Verrucomicrobiota superphylum comprises bacteria with divergent traits, epitomizing the vast biodiversity of non-model organisms that remains unexplored. Among its members, Planctomycetota and Chlamydiota exhibit unique adaptations, including variations in pe...
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Elsevier
2025-08-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225012982 |
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| author | Valentina Henriques David Moyano-Palazuelo Maria Teresa Alonso-Pascual Manuel Pazos Damien P. Devos Elena Rivas-Marin |
| author_facet | Valentina Henriques David Moyano-Palazuelo Maria Teresa Alonso-Pascual Manuel Pazos Damien P. Devos Elena Rivas-Marin |
| author_sort | Valentina Henriques |
| collection | DOAJ |
| description | Summary: The Planctomycetota-Chlamydiota-Verrucomicrobiota superphylum comprises bacteria with divergent traits, epitomizing the vast biodiversity of non-model organisms that remains unexplored. Among its members, Planctomycetota and Chlamydiota exhibit unique adaptations, including variations in peptidoglycan structure and cell division without FtsZ. Verrucomicrobiota represents the only name-giving phylum within the PVC not extensively investigated. Here, exhaustive transposon mutagenesis combined with massive sequencing reveal the essential genome of Verrucomicrobium spinosum. The essentiality report demonstrates that the dcw cluster is essential except mraW. Additionally, peptidoglycan analysis evidences that the composition and saccular arrangement are canonical, contrarily to Planctomycetota and Chlamydiota. Targeted mutagenesis for V. spinosum was also developed, thus reinforcing its status as a model for Verrucomicrobiota. Ultimately, the evolutionary divergences of the dcw cluster in PVC, combined with the previous results, suggest that its Last Common Ancestor was a Gracilicutes-like bacterium dividing by an FtsZ-mediated binary fission with peptidoglycan, from which current phyla diverged. |
| format | Article |
| id | doaj-art-338faecc4f294e4991ba2f23d197cba8 |
| institution | DOAJ |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
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| series | iScience |
| spelling | doaj-art-338faecc4f294e4991ba2f23d197cba82025-08-20T03:13:21ZengElsevieriScience2589-00422025-08-0128811303710.1016/j.isci.2025.113037Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylumValentina Henriques0David Moyano-Palazuelo1Maria Teresa Alonso-Pascual2Manuel Pazos3Damien P. Devos4Elena Rivas-Marin5Centro Andaluz de Biología del Desarrollo-CSIC, Pablo de Olavide University, Seville, SpainCentro Andaluz de Biología del Desarrollo-CSIC, Pablo de Olavide University, Seville, SpainLaboratory of Mass Spectrometry (SIdI-UAM), Autonomous University of Madrid, Madrid, SpainDepartment of Molecular Biology, Center of Molecular Biology “Severo Ochoa” (UAM-CSIC), Autonomous University of Madrid, Madrid, SpainCentro Andaluz de Biología del Desarrollo-CSIC, Pablo de Olavide University, Seville, Spain; Corresponding authorCentro Andaluz de Biología del Desarrollo-CSIC, Pablo de Olavide University, Seville, Spain; Corresponding authorSummary: The Planctomycetota-Chlamydiota-Verrucomicrobiota superphylum comprises bacteria with divergent traits, epitomizing the vast biodiversity of non-model organisms that remains unexplored. Among its members, Planctomycetota and Chlamydiota exhibit unique adaptations, including variations in peptidoglycan structure and cell division without FtsZ. Verrucomicrobiota represents the only name-giving phylum within the PVC not extensively investigated. Here, exhaustive transposon mutagenesis combined with massive sequencing reveal the essential genome of Verrucomicrobium spinosum. The essentiality report demonstrates that the dcw cluster is essential except mraW. Additionally, peptidoglycan analysis evidences that the composition and saccular arrangement are canonical, contrarily to Planctomycetota and Chlamydiota. Targeted mutagenesis for V. spinosum was also developed, thus reinforcing its status as a model for Verrucomicrobiota. Ultimately, the evolutionary divergences of the dcw cluster in PVC, combined with the previous results, suggest that its Last Common Ancestor was a Gracilicutes-like bacterium dividing by an FtsZ-mediated binary fission with peptidoglycan, from which current phyla diverged.http://www.sciencedirect.com/science/article/pii/S2589004225012982Microbial genomicsPhylogenetics |
| spellingShingle | Valentina Henriques David Moyano-Palazuelo Maria Teresa Alonso-Pascual Manuel Pazos Damien P. Devos Elena Rivas-Marin Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum iScience Microbial genomics Phylogenetics |
| title | Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum |
| title_full | Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum |
| title_fullStr | Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum |
| title_full_unstemmed | Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum |
| title_short | Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylum |
| title_sort | verrucomicrobium spinosum essential genome and the divergence of cell division in the pvc superphylum |
| topic | Microbial genomics Phylogenetics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225012982 |
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