Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology
Objective To establish a homozygous zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology so to provide an animal model for investigation of potential role of map3k15 in renal diseases. Methods 1)Analyzing the expression pattern of map3k15 in zebrafish; 2)Establishing a homozygou...
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2025-03-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-290.pdf |
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| author | JIA Chunyu, SHEN Yan, CHEN Gang, ZHENG Ke, QIN Yan, LI Xuemei |
| author_facet | JIA Chunyu, SHEN Yan, CHEN Gang, ZHENG Ke, QIN Yan, LI Xuemei |
| author_sort | JIA Chunyu, SHEN Yan, CHEN Gang, ZHENG Ke, QIN Yan, LI Xuemei |
| collection | DOAJ |
| description | Objective To establish a homozygous zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology so to provide an animal model for investigation of potential role of map3k15 in renal diseases. Methods 1)Analyzing the expression pattern of map3k15 in zebrafish; 2)Establishing a homozygous map3k15 knockout zebrafish line using CRISPR/Cas9; 3)Observing the phenotypic effects of map3k15 deficiency in zebrafish. Results 1)map3k15 was expressed in the zebrafish pronephros, and map3k15/map3k15 protein was found highly conserved across species; 2)map3k15-/- mutant zebrafish model was constructed with +2 bp mutation and +1 bp mutation; 3)map3k15 mutant zebrafish exhibited edema in yolk sac, pericardium and head during embryonic development. Conclusions A homozygous zebrafish map3k15 knockout line is successfully developed which may provide an important model for future research on the role of map3k15 in renal development and disease. |
| format | Article |
| id | doaj-art-338cbf5eddcc41e7ba711069daa4a196 |
| institution | OA Journals |
| issn | 1001-6325 |
| language | zho |
| publishDate | 2025-03-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj-art-338cbf5eddcc41e7ba711069daa4a1962025-08-20T01:50:33ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252025-03-0145329029710.16352/j.issn.1001-6325.2025.03.0290Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technologyJIA Chunyu, SHEN Yan, CHEN Gang, ZHENG Ke, QIN Yan, LI Xuemei01. Department of Nephrology, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730; ;2. Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100871, ChinaObjective To establish a homozygous zebrafish map3k15 knockout line using CRISPR/Cas9 gene editing technology so to provide an animal model for investigation of potential role of map3k15 in renal diseases. Methods 1)Analyzing the expression pattern of map3k15 in zebrafish; 2)Establishing a homozygous map3k15 knockout zebrafish line using CRISPR/Cas9; 3)Observing the phenotypic effects of map3k15 deficiency in zebrafish. Results 1)map3k15 was expressed in the zebrafish pronephros, and map3k15/map3k15 protein was found highly conserved across species; 2)map3k15-/- mutant zebrafish model was constructed with +2 bp mutation and +1 bp mutation; 3)map3k15 mutant zebrafish exhibited edema in yolk sac, pericardium and head during embryonic development. Conclusions A homozygous zebrafish map3k15 knockout line is successfully developed which may provide an important model for future research on the role of map3k15 in renal development and disease.https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-290.pdfmap3k15|crispr/cas9 gene editing technology|zebrafish|kidney development |
| spellingShingle | JIA Chunyu, SHEN Yan, CHEN Gang, ZHENG Ke, QIN Yan, LI Xuemei Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology Jichu yixue yu linchuang map3k15|crispr/cas9 gene editing technology|zebrafish|kidney development |
| title | Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology |
| title_full | Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology |
| title_fullStr | Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology |
| title_full_unstemmed | Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology |
| title_short | Establishment of a zebrafish map3k15knockout line using CRISPR/Cas9 gene editing technology |
| title_sort | establishment of a zebrafish map3k15knockout line using crispr cas9 gene editing technology |
| topic | map3k15|crispr/cas9 gene editing technology|zebrafish|kidney development |
| url | https://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2025-45-3-281-290.pdf |
| work_keys_str_mv | AT jiachunyushenyanchengangzhengkeqinyanlixuemei establishmentofazebrafishmap3k15knockoutlineusingcrisprcas9geneeditingtechnology |