Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review
Food-borne pathogens such as E. coli., Salmonella, Listeria monocytogenes, Campylobacter jejuni are the common ones that pose a significant threat to human health and therefore require quick accurate affordable detection of food pathogens. Traditional pathogen identification procedures in foods are...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225004950 |
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| author | Hiya Borah Omar Bashir Prashant Anil Pawase Imdad Ul Hoque Mondal Szilvia Várallyay Diána Ungai Béla Kovács |
| author_facet | Hiya Borah Omar Bashir Prashant Anil Pawase Imdad Ul Hoque Mondal Szilvia Várallyay Diána Ungai Béla Kovács |
| author_sort | Hiya Borah |
| collection | DOAJ |
| description | Food-borne pathogens such as E. coli., Salmonella, Listeria monocytogenes, Campylobacter jejuni are the common ones that pose a significant threat to human health and therefore require quick accurate affordable detection of food pathogens. Traditional pathogen identification procedures in foods are laborious and expensive and require specialized equipments. Magnetic Relaxation Switching (MRS) biosensors, which work by monitoring changes on dispersion, aggregation, or concentration change of magnetic nanoparticle (MNPs), have emerged as an potential alternative. Therefore, MNPs have gained wide applications due to the typical magnetic properties that may efficiently enhance the target, separate, and capture with high sensitivity. However, traditional MRS biosensors are often insensitive and unstable. The review, therefore, focuses on the latest development of CRISPR-Cas12a-assisted MRS biosensors for improving food safety monitoring. The CRISPR-Cas12a system enhances pathogen detection by recognizing and cleaving target sequence with high precision. Lastly, the review sums up the improvements of different biosensors in comparison with conventional biosensors and shows the use of MNPs in the identification process of various pathogens. |
| format | Article |
| id | doaj-art-337f1fb2dfce4489bd3c89e6fc33c916 |
| institution | Kabale University |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-337f1fb2dfce4489bd3c89e6fc33c9162025-08-20T03:36:18ZengElsevierApplied Food Research2772-50222025-12-015210119010.1016/j.afres.2025.101190Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive reviewHiya Borah0Omar Bashir1Prashant Anil Pawase2Imdad Ul Hoque Mondal3Szilvia Várallyay4Diána Ungai5Béla Kovács6Department of Food Technology and Nutrition, Lovely Professional University, Phagwara, 144402, Punjab, IndiaDepartment of Food Technology and Nutrition, Lovely Professional University, Phagwara, 144402, Punjab, India; Corresponding authors.School of Food Technology, Maharashtra Institute of Technology, Art design and Technology University, Pune, Maharashtra, IndiaDepartment of Food Technology and Nutrition, Lovely Professional University, Phagwara, 144402, Punjab, IndiaFaculty of Agriculture, Food Science and Environmental Management, Institute of Food Science, University of Debrecen, 4032, Debrecen, HungaryFaculty of Agriculture, Food Science and Environmental Management, Institute of Food Science, University of Debrecen, 4032, Debrecen, HungaryFaculty of Agriculture, Food Science and Environmental Management, Institute of Food Science, University of Debrecen, 4032, Debrecen, Hungary; Corresponding authors.Food-borne pathogens such as E. coli., Salmonella, Listeria monocytogenes, Campylobacter jejuni are the common ones that pose a significant threat to human health and therefore require quick accurate affordable detection of food pathogens. Traditional pathogen identification procedures in foods are laborious and expensive and require specialized equipments. Magnetic Relaxation Switching (MRS) biosensors, which work by monitoring changes on dispersion, aggregation, or concentration change of magnetic nanoparticle (MNPs), have emerged as an potential alternative. Therefore, MNPs have gained wide applications due to the typical magnetic properties that may efficiently enhance the target, separate, and capture with high sensitivity. However, traditional MRS biosensors are often insensitive and unstable. The review, therefore, focuses on the latest development of CRISPR-Cas12a-assisted MRS biosensors for improving food safety monitoring. The CRISPR-Cas12a system enhances pathogen detection by recognizing and cleaving target sequence with high precision. Lastly, the review sums up the improvements of different biosensors in comparison with conventional biosensors and shows the use of MNPs in the identification process of various pathogens.http://www.sciencedirect.com/science/article/pii/S2772502225004950Magnetic Relaxation Switch biosensors (MRS)Magnetic nano-particles (MNP)PathogenClustered regularly interspaced short palindromic repeats (CRISPR) |
| spellingShingle | Hiya Borah Omar Bashir Prashant Anil Pawase Imdad Ul Hoque Mondal Szilvia Várallyay Diána Ungai Béla Kovács Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review Applied Food Research Magnetic Relaxation Switch biosensors (MRS) Magnetic nano-particles (MNP) Pathogen Clustered regularly interspaced short palindromic repeats (CRISPR) |
| title | Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review |
| title_full | Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review |
| title_fullStr | Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review |
| title_full_unstemmed | Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review |
| title_short | Exploring the versatility of Magnetic Relaxation Switching (MRS) and CRISPR-based biosensors for the detection of foodborne pathogens; a comprehensive review |
| title_sort | exploring the versatility of magnetic relaxation switching mrs and crispr based biosensors for the detection of foodborne pathogens a comprehensive review |
| topic | Magnetic Relaxation Switch biosensors (MRS) Magnetic nano-particles (MNP) Pathogen Clustered regularly interspaced short palindromic repeats (CRISPR) |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225004950 |
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