Autophagy is induced during plant grafting to promote wound healing
Abstract Grafting is an agricultural technique that joins tissues from different plants to obtain useful rootstock traits. However, cellular processes involved in joint tissue repair remain poorly understood. We analyzed Nicotiana benthamiana (Nb) and Arabidopsis thaliana (At) interfamily heterograf...
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Nature Portfolio
2025-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-58519-6 |
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| author | Ken-ichi Kurotani Daiki Shinozaki Kentaro Okada Ryo Tabata Yaichi Kawakatsu Ryohei Sugita Yuki Utsugi Koji Okayasu Moe Mori Keitaro Tanoi Yumi Goto Mayuko Sato Kiminori Toyooka Kohki Yoshimoto Michitaka Notaguchi |
| author_facet | Ken-ichi Kurotani Daiki Shinozaki Kentaro Okada Ryo Tabata Yaichi Kawakatsu Ryohei Sugita Yuki Utsugi Koji Okayasu Moe Mori Keitaro Tanoi Yumi Goto Mayuko Sato Kiminori Toyooka Kohki Yoshimoto Michitaka Notaguchi |
| author_sort | Ken-ichi Kurotani |
| collection | DOAJ |
| description | Abstract Grafting is an agricultural technique that joins tissues from different plants to obtain useful rootstock traits. However, cellular processes involved in joint tissue repair remain poorly understood. We analyzed Nicotiana benthamiana (Nb) and Arabidopsis thaliana (At) interfamily heterografting as a high-stress model and At homografting as a low-stress model. Transmission electron micrographs reveal the formation of autophagic structures in cells near the graft boundary over a long period in Nb/At interfamily grafts and in a short period of a few days in At homografts. Using a GFP-ATG8 marker line, the autophagosomes were observed in the cells near the graft boundary, especially on the scion side, where nutrient depletion occurred. Grafting of At autophagy-defective mutants decreases grafting success rates and post-grafting growth. NbATG5 knockdown suppresses graft establishment in Nb/At interfamily heterografts. Moreover, At autophagy-defective mutants show reduced callus formation directed to wounds under the nutrient-deficient conditions. These results suggest that autophagy is induced during grafting, promoting callus formation and contributing to tissue connectivity. |
| format | Article |
| id | doaj-art-33792dc636fc4e90bd1728bdc192ff48 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-33792dc636fc4e90bd1728bdc192ff482025-08-20T03:06:54ZengNature PortfolioNature Communications2041-17232025-04-0116111410.1038/s41467-025-58519-6Autophagy is induced during plant grafting to promote wound healingKen-ichi Kurotani0Daiki Shinozaki1Kentaro Okada2Ryo Tabata3Yaichi Kawakatsu4Ryohei Sugita5Yuki Utsugi6Koji Okayasu7Moe Mori8Keitaro Tanoi9Yumi Goto10Mayuko Sato11Kiminori Toyooka12Kohki Yoshimoto13Michitaka Notaguchi14Bioscience and Biotechnology Center, Nagoya UniversityDepartment of Life Sciences, School of Agriculture, Meiji UniversityBioscience and Biotechnology Center, Nagoya UniversityGraduate School of Bioagricultural Sciences, Nagoya UniversityBioscience and Biotechnology Center, Nagoya UniversityIsotope Facility for Agricultural Education and Research, Graduate School of Agricultural and Life Sciences, The University of TokyoDepartment of Life Sciences, School of Agriculture, Meiji UniversityGraduate School of Bioagricultural Sciences, Nagoya UniversityGraduate School of Bioagricultural Sciences, Nagoya UniversityIsotope Facility for Agricultural Education and Research, Graduate School of Agricultural and Life Sciences, The University of TokyoCenter for Sustainable Resource Science, RIKENCenter for Sustainable Resource Science, RIKENCenter for Sustainable Resource Science, RIKENDepartment of Life Sciences, School of Agriculture, Meiji UniversityBioscience and Biotechnology Center, Nagoya UniversityAbstract Grafting is an agricultural technique that joins tissues from different plants to obtain useful rootstock traits. However, cellular processes involved in joint tissue repair remain poorly understood. We analyzed Nicotiana benthamiana (Nb) and Arabidopsis thaliana (At) interfamily heterografting as a high-stress model and At homografting as a low-stress model. Transmission electron micrographs reveal the formation of autophagic structures in cells near the graft boundary over a long period in Nb/At interfamily grafts and in a short period of a few days in At homografts. Using a GFP-ATG8 marker line, the autophagosomes were observed in the cells near the graft boundary, especially on the scion side, where nutrient depletion occurred. Grafting of At autophagy-defective mutants decreases grafting success rates and post-grafting growth. NbATG5 knockdown suppresses graft establishment in Nb/At interfamily heterografts. Moreover, At autophagy-defective mutants show reduced callus formation directed to wounds under the nutrient-deficient conditions. These results suggest that autophagy is induced during grafting, promoting callus formation and contributing to tissue connectivity.https://doi.org/10.1038/s41467-025-58519-6 |
| spellingShingle | Ken-ichi Kurotani Daiki Shinozaki Kentaro Okada Ryo Tabata Yaichi Kawakatsu Ryohei Sugita Yuki Utsugi Koji Okayasu Moe Mori Keitaro Tanoi Yumi Goto Mayuko Sato Kiminori Toyooka Kohki Yoshimoto Michitaka Notaguchi Autophagy is induced during plant grafting to promote wound healing Nature Communications |
| title | Autophagy is induced during plant grafting to promote wound healing |
| title_full | Autophagy is induced during plant grafting to promote wound healing |
| title_fullStr | Autophagy is induced during plant grafting to promote wound healing |
| title_full_unstemmed | Autophagy is induced during plant grafting to promote wound healing |
| title_short | Autophagy is induced during plant grafting to promote wound healing |
| title_sort | autophagy is induced during plant grafting to promote wound healing |
| url | https://doi.org/10.1038/s41467-025-58519-6 |
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