The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium
Background: There are conflicting findings regarding the effect of various training modalities on the cardiovascular disease mechanisms induced by undesirable modifications in extracellular matrix (ECM) factors such as MMP-9 and TIMP-1.Aim: This study was conducted to investigate the effect of diffe...
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University of Tehran
2023-01-01
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| Series: | Sport Sciences and Health Research |
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| Online Access: | https://sshr.ut.ac.ir/article_94789_77d84850aa53f8abb5c7bce8efd8e4bd.pdf |
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| author | Mehran Ghahremani Elham Pourrezaei Saeed Naghibi Afshar Jafari |
| author_facet | Mehran Ghahremani Elham Pourrezaei Saeed Naghibi Afshar Jafari |
| author_sort | Mehran Ghahremani |
| collection | DOAJ |
| description | Background: There are conflicting findings regarding the effect of various training modalities on the cardiovascular disease mechanisms induced by undesirable modifications in extracellular matrix (ECM) factors such as MMP-9 and TIMP-1.Aim: This study was conducted to investigate the effect of different training protocols on the MMP-9 and TIMP-1 gene expression in the myocardium of Wistar male rats.Materials and Methods: Forty-eight adult Wistar male rats (aged: 8 weeks) were randomly allocated into two control (Con8w/base, n=8; and Con16w/end control, n=8) and four training groups (MICT, n=8; PICT, n=8; HIIT, n=8; and MIST, n=8). The training protocols consisted of 8 weeks (5 days/week) of Moderate-Intensity, Continuous Training (MICT: 37 min running with 65% vVO2max), Moderate-Intensity, Swimming Training (MIST: 30 min free-swimming), Progressive-Intensity, Continuous Training (PICT: 28 min running with a progressive increase in the incline of the treadmill by 2% per week), and High-Intensity Interval Training (HIIT: four running bouts 4 min with 90 to 100% vVO2max + 3 min with 50-65% vVO2max). Myocardial samples were isolated 24 hours after the last training session. Gene expression was determined using the real-time PCR method. All data (M±SD) were analyzed by one-way ANOVA and Tukey's post-hoc tests at α ≤0.05.Results: There were significant differences between MMP-9 and TIMP-1 expression in the animals myocardium after different exercise training protocols (P<0.01). The lowest and highest MMP-9/TIMP-1 ratios were in the HIIT and MICT protocols, respectively.Conclusion: According to our findings, the HIIT protocol may play a more influential role in enhancing myocardial ECM-remodeling and fibrosis indicators during lifespan than the other exercise protocols. |
| format | Article |
| id | doaj-art-3377dcd4fd4e43af827e3486c3f154fe |
| institution | Kabale University |
| issn | 2981-0205 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Sport Sciences and Health Research |
| spelling | doaj-art-3377dcd4fd4e43af827e3486c3f154fe2025-08-20T03:25:15ZengUniversity of TehranSport Sciences and Health Research2981-02052023-01-01151455210.22059/sshr.2023.361500.110694789The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardiumMehran Ghahremani0Elham Pourrezaei1Saeed Naghibi2Afshar Jafari3Department of Physical Education and Sport Sciences, Physical Education and Sport Sciences, Payame Noor University (Alborz), Karaj, Iran.Department of Physical Education and Sport Sciences, Physical Education and Sport Sciences, Payame Noor University (Alborz), Karaj, Iran.Department of Physical Education and Sport Sciences, Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran.Background: There are conflicting findings regarding the effect of various training modalities on the cardiovascular disease mechanisms induced by undesirable modifications in extracellular matrix (ECM) factors such as MMP-9 and TIMP-1.Aim: This study was conducted to investigate the effect of different training protocols on the MMP-9 and TIMP-1 gene expression in the myocardium of Wistar male rats.Materials and Methods: Forty-eight adult Wistar male rats (aged: 8 weeks) were randomly allocated into two control (Con8w/base, n=8; and Con16w/end control, n=8) and four training groups (MICT, n=8; PICT, n=8; HIIT, n=8; and MIST, n=8). The training protocols consisted of 8 weeks (5 days/week) of Moderate-Intensity, Continuous Training (MICT: 37 min running with 65% vVO2max), Moderate-Intensity, Swimming Training (MIST: 30 min free-swimming), Progressive-Intensity, Continuous Training (PICT: 28 min running with a progressive increase in the incline of the treadmill by 2% per week), and High-Intensity Interval Training (HIIT: four running bouts 4 min with 90 to 100% vVO2max + 3 min with 50-65% vVO2max). Myocardial samples were isolated 24 hours after the last training session. Gene expression was determined using the real-time PCR method. All data (M±SD) were analyzed by one-way ANOVA and Tukey's post-hoc tests at α ≤0.05.Results: There were significant differences between MMP-9 and TIMP-1 expression in the animals myocardium after different exercise training protocols (P<0.01). The lowest and highest MMP-9/TIMP-1 ratios were in the HIIT and MICT protocols, respectively.Conclusion: According to our findings, the HIIT protocol may play a more influential role in enhancing myocardial ECM-remodeling and fibrosis indicators during lifespan than the other exercise protocols.https://sshr.ut.ac.ir/article_94789_77d84850aa53f8abb5c7bce8efd8e4bd.pdfangiogenesisexercise modalitiesheart fibrosisgene expressionmatrix metalloproteinase |
| spellingShingle | Mehran Ghahremani Elham Pourrezaei Saeed Naghibi Afshar Jafari The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium Sport Sciences and Health Research angiogenesis exercise modalities heart fibrosis gene expression matrix metalloproteinase |
| title | The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium |
| title_full | The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium |
| title_fullStr | The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium |
| title_full_unstemmed | The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium |
| title_short | The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium |
| title_sort | effect of different exercise training protocols on mmp 9 and timp 1 gene expression in rat myocardium |
| topic | angiogenesis exercise modalities heart fibrosis gene expression matrix metalloproteinase |
| url | https://sshr.ut.ac.ir/article_94789_77d84850aa53f8abb5c7bce8efd8e4bd.pdf |
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