Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses
As the largest organic carbon input in the agroecosystems, crop residues can increase soil carbon sequestration and crop production in greenhouse vegetable fields (GVFs). However, the soil microbiological mechanisms driving straw decomposition in GVFs under different incubation temperatures and fert...
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Frontiers Media S.A.
2024-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1486817/full |
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| author | Long Ma Ruonan Li Haoan Luan Jiwei Tang Liying Wang Tengfei Guo Shaowen Huang |
| author_facet | Long Ma Ruonan Li Haoan Luan Jiwei Tang Liying Wang Tengfei Guo Shaowen Huang |
| author_sort | Long Ma |
| collection | DOAJ |
| description | As the largest organic carbon input in the agroecosystems, crop residues can increase soil carbon sequestration and crop production in greenhouse vegetable fields (GVFs). However, the soil microbiological mechanisms driving straw decomposition in GVFs under different incubation temperatures and fertilization treatments are not clear. Thus, soil samples were collected from a long-term field experiment included chemical fertilizer application alone (CF), 2/4 fertilizer N+2/4 organic fertilizer N (CM), 2/4 fertilizer N+1/4 organic fertilizer N+1/4 straw N (CMS), 2/4 fertilizer N+2/4 straw N (CS), and incubated with 13C-labeled straw at different temperatures (15, 25, and 35°C) for 60 days. Organic-amended treatments (CM, CMS, and CS), especially CMS treatment, increased soil bacterial Alpha diversity before and after straw addition. Straw decomposition process was dominated by soil Proteobacteria, Actinobacteria, and Firmicutes for each treatments. The effect of incubation temperature on soil microbial community composition was higher than that of fertilization treatments. Soil Alphaproteobacteria and Actinomycetia were the most predominant class involved in straw decomposition. Gammaproteobacteria (Pseudomonas, Steroidobacter, Acidibacter, and Arenimonas) were the unique and predominant class involved in straw decomposition at medium and high temperatures as well as in the straw-amended treatments. Organic-amended treatments, especially straw-amended treatments, increased the relative abundance of glycosyl transferases (GT) and auxiliary activities (AA). Alphaproteobacteria, Actinomycetia, and Gammaproteobacteria had higher relative contribution to carbohydrase genes. In summary, the long-term organic-amended treatments altered the structure of soil microbial communities and increased soil bacterial diversity, with the CMS having a greater potential to enhance resistance to external environmental changes. Soil Alphaproteobacteria and Actinomycetia were responsible for the dominance of straw decomposition, and Gammaproteobacteria may be responsible for the acceleration of straw decomposition. Fertilization treatments promote straw decomposition by increasing the abundance of indicator bacterial groups involved in straw decomposition, which is important for isolating key microbial species involved in straw decomposition under global warming. |
| format | Article |
| id | doaj-art-3376d1d238d04f228e714faad0af8f43 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-3376d1d238d04f228e714faad0af8f432025-08-20T02:11:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-10-011510.3389/fpls.2024.14868171486817Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responsesLong Ma0Ruonan Li1Haoan Luan2Jiwei Tang3Liying Wang4Tengfei Guo5Shaowen Huang6State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, ChinaCollege of Forestry, Hebei Agricultural University, Baoding, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Agricultural Resources and Environment, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, ChinaInstitution of Plant Nutrition and Environmental Resources, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaAs the largest organic carbon input in the agroecosystems, crop residues can increase soil carbon sequestration and crop production in greenhouse vegetable fields (GVFs). However, the soil microbiological mechanisms driving straw decomposition in GVFs under different incubation temperatures and fertilization treatments are not clear. Thus, soil samples were collected from a long-term field experiment included chemical fertilizer application alone (CF), 2/4 fertilizer N+2/4 organic fertilizer N (CM), 2/4 fertilizer N+1/4 organic fertilizer N+1/4 straw N (CMS), 2/4 fertilizer N+2/4 straw N (CS), and incubated with 13C-labeled straw at different temperatures (15, 25, and 35°C) for 60 days. Organic-amended treatments (CM, CMS, and CS), especially CMS treatment, increased soil bacterial Alpha diversity before and after straw addition. Straw decomposition process was dominated by soil Proteobacteria, Actinobacteria, and Firmicutes for each treatments. The effect of incubation temperature on soil microbial community composition was higher than that of fertilization treatments. Soil Alphaproteobacteria and Actinomycetia were the most predominant class involved in straw decomposition. Gammaproteobacteria (Pseudomonas, Steroidobacter, Acidibacter, and Arenimonas) were the unique and predominant class involved in straw decomposition at medium and high temperatures as well as in the straw-amended treatments. Organic-amended treatments, especially straw-amended treatments, increased the relative abundance of glycosyl transferases (GT) and auxiliary activities (AA). Alphaproteobacteria, Actinomycetia, and Gammaproteobacteria had higher relative contribution to carbohydrase genes. In summary, the long-term organic-amended treatments altered the structure of soil microbial communities and increased soil bacterial diversity, with the CMS having a greater potential to enhance resistance to external environmental changes. Soil Alphaproteobacteria and Actinomycetia were responsible for the dominance of straw decomposition, and Gammaproteobacteria may be responsible for the acceleration of straw decomposition. Fertilization treatments promote straw decomposition by increasing the abundance of indicator bacterial groups involved in straw decomposition, which is important for isolating key microbial species involved in straw decomposition under global warming.https://www.frontiersin.org/articles/10.3389/fpls.2024.1486817/fullgreenhouse vegetable soilsstraw decompositionlong-term different fertilization treatmentsincubation temperaturesDNA-SIPhigh-throughput and metagenomic sequencing |
| spellingShingle | Long Ma Ruonan Li Haoan Luan Jiwei Tang Liying Wang Tengfei Guo Shaowen Huang Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses Frontiers in Plant Science greenhouse vegetable soils straw decomposition long-term different fertilization treatments incubation temperatures DNA-SIP high-throughput and metagenomic sequencing |
| title | Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses |
| title_full | Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses |
| title_fullStr | Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses |
| title_full_unstemmed | Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses |
| title_short | Impacts of long-term different fertilization regimes on microbial utilization of straw-derived carbon in greenhouse vegetable soils: insights from its ecophysiological roles and temperature responses |
| title_sort | impacts of long term different fertilization regimes on microbial utilization of straw derived carbon in greenhouse vegetable soils insights from its ecophysiological roles and temperature responses |
| topic | greenhouse vegetable soils straw decomposition long-term different fertilization treatments incubation temperatures DNA-SIP high-throughput and metagenomic sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1486817/full |
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