Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L.
The aim of this work is the isolation, identification and evaluation of the antibacterial activity of endophytic fungi from the Algerian medicinal plant Marrubium vulgare L. The isolation of endophytic fungi is carried out by the method of sterilization of surface to eliminate epiphytes then incubat...
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| Language: | English |
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Hasan Eleroğlu
2022-04-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5178 |
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| author | Abderrahim Benslama Warda Nouiri |
| author_facet | Abderrahim Benslama Warda Nouiri |
| author_sort | Abderrahim Benslama |
| collection | DOAJ |
| description | The aim of this work is the isolation, identification and evaluation of the antibacterial activity of endophytic fungi from the Algerian medicinal plant Marrubium vulgare L. The isolation of endophytic fungi is carried out by the method of sterilization of surface to eliminate epiphytes then incubation of the fragments treated according to a precise protocol. The identification of the grown isolates allowed us to obtain three fungal strains belonging to three genera: Fusarium, Alternaria and Chaetomium, which are generally of the Deuteromycete phylum. The antibacterial capacity of the fungi was tested against five human pathogenic bacteruim using the agar-fungi disk diffusion method. With an inhibition zone (IZ) spanning from 7.5 to 25 mm, all isolated fungal strains showed antibacterial activity against at least one of the bacteria tested.. However, Fusarium sp has the highest antibacterial activity with an IZ of 19 and 24 mm against S. aureus and B. subtilis, respectively. Finally, our results clearly confirm that the medicinal plant M. vulgare L. presents a reservoir of endophytic fungi, which can be used in various fields, especially pharmaceutical fields. |
| format | Article |
| id | doaj-art-336f81f6f8ac4f4eacba6c415024b503 |
| institution | DOAJ |
| issn | 2148-127X |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-336f81f6f8ac4f4eacba6c415024b5032025-08-20T03:18:30ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2022-04-0110476677010.24925/turjaf.v10i4.766-770.51782460Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L.Abderrahim Benslama0Warda Nouiri1Department of Biochemistry and Microbiology, University of M’sila, Faculty of Sciences, M’silaDepartment of Biochemistry and Microbiology, University of M’sila, Faculty of Sciences, M’silaThe aim of this work is the isolation, identification and evaluation of the antibacterial activity of endophytic fungi from the Algerian medicinal plant Marrubium vulgare L. The isolation of endophytic fungi is carried out by the method of sterilization of surface to eliminate epiphytes then incubation of the fragments treated according to a precise protocol. The identification of the grown isolates allowed us to obtain three fungal strains belonging to three genera: Fusarium, Alternaria and Chaetomium, which are generally of the Deuteromycete phylum. The antibacterial capacity of the fungi was tested against five human pathogenic bacteruim using the agar-fungi disk diffusion method. With an inhibition zone (IZ) spanning from 7.5 to 25 mm, all isolated fungal strains showed antibacterial activity against at least one of the bacteria tested.. However, Fusarium sp has the highest antibacterial activity with an IZ of 19 and 24 mm against S. aureus and B. subtilis, respectively. Finally, our results clearly confirm that the medicinal plant M. vulgare L. presents a reservoir of endophytic fungi, which can be used in various fields, especially pharmaceutical fields.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5178antibacterial activityendophytic fungimarrubium vulgare lmedicinal plants |
| spellingShingle | Abderrahim Benslama Warda Nouiri Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L. Turkish Journal of Agriculture: Food Science and Technology antibacterial activity endophytic fungi marrubium vulgare l medicinal plants |
| title | Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L. |
| title_full | Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L. |
| title_fullStr | Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L. |
| title_full_unstemmed | Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L. |
| title_short | Isolation, Characterization and Antibacterial Activity of Endophytic Fungi from Marrubium vulgare L. |
| title_sort | isolation characterization and antibacterial activity of endophytic fungi from marrubium vulgare l |
| topic | antibacterial activity endophytic fungi marrubium vulgare l medicinal plants |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5178 |
| work_keys_str_mv | AT abderrahimbenslama isolationcharacterizationandantibacterialactivityofendophyticfungifrommarrubiumvulgarel AT wardanouiri isolationcharacterizationandantibacterialactivityofendophyticfungifrommarrubiumvulgarel |