Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture
IntroductionBone, as both a crucial organ with mechanical support and immune regulatory functions, profoundly influencing the growth and development of fish in aquaculture systems. Deer antler and its aqueous extract Cervi Cornus Colla (CCC), are natural medicinal substances known for their bone-pro...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1620056/full |
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| author | Fengxia Xu Limin Dong Limin Dong Chengxue Qin Yanru Ren Liang Kong Mengru Zhang Haitao Du Qing Xia Cheng Wang Ping Wang Ping Wang |
| author_facet | Fengxia Xu Limin Dong Limin Dong Chengxue Qin Yanru Ren Liang Kong Mengru Zhang Haitao Du Qing Xia Cheng Wang Ping Wang Ping Wang |
| author_sort | Fengxia Xu |
| collection | DOAJ |
| description | IntroductionBone, as both a crucial organ with mechanical support and immune regulatory functions, profoundly influencing the growth and development of fish in aquaculture systems. Deer antler and its aqueous extract Cervi Cornus Colla (CCC), are natural medicinal substances known for their bone-protective properties.MethodsThis study investigated the osteoprotective effects of CCC against lipopolysaccharide (LPS)-induced injury to fish bones. An LPS-mediated zebrafish skeletal injury model was established and evaluated using Alcian Blue/Alizarin Red staining, fluorescence tracing, ELISA, and qPCR.ResultsThe median lethal concentration (LC50) of CCC in zebrafish was 206.3 mg/mL, and staining revealed that CCC reversed LPS-induced reduction in cartilage and bone formation in a dose-dependent manner. In Tg (mpeg1: EGFP) zebrafish, CCC treatment significantly decreased macrophages-associated green fluorescence, suggesting that the extract suppressed the activation of macrophages caused by LPS. CCC also reduced the production of several inflammatory factors (IL-6, IL- 1β, and NO). Furthermore, qRT-PCR confirmed CCC restored COL2α1 and Sox9a mRNA levels. Additional studies revealed CCC inhibits LPS-induced activation of the TGF-β/Smad3 signaling pathway.DiscussionThis study demonstrates that CCC can alleviate zebrafish bone injury, thereby providing an experimental basis for its application in the prevention and treatment of bone diseases in farmed fish. |
| format | Article |
| id | doaj-art-336592e9a58e46b59edba8151b954bfa |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj-art-336592e9a58e46b59edba8151b954bfa2025-08-22T04:10:33ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-08-011210.3389/fmars.2025.16200561620056Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquacultureFengxia Xu0Limin Dong1Limin Dong2Chengxue Qin3Yanru Ren4Liang Kong5Mengru Zhang6Haitao Du7Qing Xia8Cheng Wang9Ping Wang10Ping Wang11Shandong Hanfang Pharmaceutical Co. LTD, Jinan, Shandong, ChinaInstitute of Traditional Chinese Medicine Pharmacology, Shandong Academy of Chinese Medicine, Jinan, Shandong, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaShandong Hanfang Pharmaceutical Co. LTD, Jinan, Shandong, ChinaShandong Hanfang Pharmaceutical Co. LTD, Jinan, Shandong, ChinaShandong Hanfang Pharmaceutical Co. LTD, Jinan, Shandong, ChinaInstitute of Traditional Chinese Medicine Pharmacology, Shandong Academy of Chinese Medicine, Jinan, Shandong, ChinaInstitute of Traditional Chinese Medicine Pharmacology, Shandong Academy of Chinese Medicine, Jinan, Shandong, ChinaBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaInstitute of Traditional Chinese Medicine Pharmacology, Shandong Academy of Chinese Medicine, Jinan, Shandong, ChinaInstitute of Traditional Chinese Medicine Pharmacology, Shandong Academy of Chinese Medicine, Jinan, Shandong, ChinaCollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong, ChinaIntroductionBone, as both a crucial organ with mechanical support and immune regulatory functions, profoundly influencing the growth and development of fish in aquaculture systems. Deer antler and its aqueous extract Cervi Cornus Colla (CCC), are natural medicinal substances known for their bone-protective properties.MethodsThis study investigated the osteoprotective effects of CCC against lipopolysaccharide (LPS)-induced injury to fish bones. An LPS-mediated zebrafish skeletal injury model was established and evaluated using Alcian Blue/Alizarin Red staining, fluorescence tracing, ELISA, and qPCR.ResultsThe median lethal concentration (LC50) of CCC in zebrafish was 206.3 mg/mL, and staining revealed that CCC reversed LPS-induced reduction in cartilage and bone formation in a dose-dependent manner. In Tg (mpeg1: EGFP) zebrafish, CCC treatment significantly decreased macrophages-associated green fluorescence, suggesting that the extract suppressed the activation of macrophages caused by LPS. CCC also reduced the production of several inflammatory factors (IL-6, IL- 1β, and NO). Furthermore, qRT-PCR confirmed CCC restored COL2α1 and Sox9a mRNA levels. Additional studies revealed CCC inhibits LPS-induced activation of the TGF-β/Smad3 signaling pathway.DiscussionThis study demonstrates that CCC can alleviate zebrafish bone injury, thereby providing an experimental basis for its application in the prevention and treatment of bone diseases in farmed fish.https://www.frontiersin.org/articles/10.3389/fmars.2025.1620056/fullaquacultureCervi Cornus Collazebrafishboneimmunity |
| spellingShingle | Fengxia Xu Limin Dong Limin Dong Chengxue Qin Yanru Ren Liang Kong Mengru Zhang Haitao Du Qing Xia Cheng Wang Ping Wang Ping Wang Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture Frontiers in Marine Science aquaculture Cervi Cornus Colla zebrafish bone immunity |
| title | Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture |
| title_full | Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture |
| title_fullStr | Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture |
| title_full_unstemmed | Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture |
| title_short | Therapeutic potential of Cervi Cornus Colla in zebrafish bone injury: implications for aquaculture |
| title_sort | therapeutic potential of cervi cornus colla in zebrafish bone injury implications for aquaculture |
| topic | aquaculture Cervi Cornus Colla zebrafish bone immunity |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1620056/full |
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