DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN
Photooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX...
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2019-02-01
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| Series: | Тонкие химические технологии |
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| Online Access: | https://www.finechem-mirea.ru/jour/article/view/185 |
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| author | V. V. Skarga E. V. Nevezhin A. А. Matrosov V. V. Negrebetsky M. V. Malakhov |
| author_facet | V. V. Skarga E. V. Nevezhin A. А. Matrosov V. V. Negrebetsky M. V. Malakhov |
| author_sort | V. V. Skarga |
| collection | DOAJ |
| description | Photooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX reagent with 10× content of Xylenol Orange, modified for quantitative analysis of up to 50 μM of hydroperoxides in aqueous phase was used in experiments. During photooxidation of 0.1 mM psoralen in phosphate buffer solution, hydroperoxide production increases with dose of UVA irradiation (~2.5 μM eq. of H2O2 for dose of 252 kJ/m2 and ~11 μM eq. of H2O2 for dose of 1512 kJ/m2) and reaches ~16.5 μM eq. of H2O2 at the highest dose investigated (3024 kJ/m2). A comparison of kinetics of psoralen photolysis and hydroperoxide generation allows us to suggest that generation of hydroperoxide results from the secondary photochemical processes involving psoralen photoproducts, presumably from photoinduced autooxidation of aldehydic photoproducts of psoralen. |
| format | Article |
| id | doaj-art-33632c745826421eafa5bf035b66927f |
| institution | Kabale University |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| publishDate | 2019-02-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj-art-33632c745826421eafa5bf035b66927f2025-08-20T03:42:45ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752019-02-01141323810.32362/2410-6593-2019-14-1-32-38185DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALENV. V. Skarga0E. V. Nevezhin1A. А. Matrosov2V. V. Negrebetsky3M. V. Malakhov4Pirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityPirogov Russian National Research Medical UniversityPhotooxidized psoralen solutions possess a variety of biological effects, which implementation mechanism may presumably involve hydroperoxides. Here, the hydroperoxide content in photooxidized psoralen solutions was assessed using photometric FOX assay (from Ferrous Oxidation + Xylenol Orange). FOX reagent with 10× content of Xylenol Orange, modified for quantitative analysis of up to 50 μM of hydroperoxides in aqueous phase was used in experiments. During photooxidation of 0.1 mM psoralen in phosphate buffer solution, hydroperoxide production increases with dose of UVA irradiation (~2.5 μM eq. of H2O2 for dose of 252 kJ/m2 and ~11 μM eq. of H2O2 for dose of 1512 kJ/m2) and reaches ~16.5 μM eq. of H2O2 at the highest dose investigated (3024 kJ/m2). A comparison of kinetics of psoralen photolysis and hydroperoxide generation allows us to suggest that generation of hydroperoxide results from the secondary photochemical processes involving psoralen photoproducts, presumably from photoinduced autooxidation of aldehydic photoproducts of psoralen.https://www.finechem-mirea.ru/jour/article/view/185psoralenphotooxidationhydroperoxideshydrogen peroxidexylenol orangespectrophotometr |
| spellingShingle | V. V. Skarga E. V. Nevezhin A. А. Matrosov V. V. Negrebetsky M. V. Malakhov DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN Тонкие химические технологии psoralen photooxidation hydroperoxides hydrogen peroxide xylenol orange spectrophotometr |
| title | DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN |
| title_full | DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN |
| title_fullStr | DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN |
| title_full_unstemmed | DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN |
| title_short | DETECTION OF HYDROPEROXIDES IN SOLUTIONS OF PHOTOOXIDIZED PSORALEN |
| title_sort | detection of hydroperoxides in solutions of photooxidized psoralen |
| topic | psoralen photooxidation hydroperoxides hydrogen peroxide xylenol orange spectrophotometr |
| url | https://www.finechem-mirea.ru/jour/article/view/185 |
| work_keys_str_mv | AT vvskarga detectionofhydroperoxidesinsolutionsofphotooxidizedpsoralen AT evnevezhin detectionofhydroperoxidesinsolutionsofphotooxidizedpsoralen AT aamatrosov detectionofhydroperoxidesinsolutionsofphotooxidizedpsoralen AT vvnegrebetsky detectionofhydroperoxidesinsolutionsofphotooxidizedpsoralen AT mvmalakhov detectionofhydroperoxidesinsolutionsofphotooxidizedpsoralen |