Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents

In order to establish an efficient process for the pretreatment of lignocellulosic biomass to deconstruct the complex structure and improve enzymatic hydrolysis efficiency of polysaccharide fractions, choline chloride(ChCl) and glycerol(Gly) were used to synthesize green deep eutectic solvent(DES) f...

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Main Authors: Jiabin WANG, Shijie WANG, Qianyue YANG, Rui ZHANG, Ruihong GAO, Rongling YANG, Hongzhen LUO
Format: Article
Language:zho
Published: Hebei University of Science and Technology 2025-06-01
Series:Journal of Hebei University of Science and Technology
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Online Access:https://xuebao.hebust.edu.cn/hbkjdx/article/pdf/b202503006?st=article_issue
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author Jiabin WANG
Shijie WANG
Qianyue YANG
Rui ZHANG
Ruihong GAO
Rongling YANG
Hongzhen LUO
author_facet Jiabin WANG
Shijie WANG
Qianyue YANG
Rui ZHANG
Ruihong GAO
Rongling YANG
Hongzhen LUO
author_sort Jiabin WANG
collection DOAJ
description In order to establish an efficient process for the pretreatment of lignocellulosic biomass to deconstruct the complex structure and improve enzymatic hydrolysis efficiency of polysaccharide fractions, choline chloride(ChCl) and glycerol(Gly) were used to synthesize green deep eutectic solvent(DES) for corn stover pretreatment.The deconstruction effects of DES on corn stover under different synthesis conditions were compared, and the chemical reagents were added to enhance pretreatment. The results indicate that, pretreatment at 120 ℃ for 3 h by DES (at a molar ratio of 1∶2) with 1% H2SO4 addition improves the glucan content from 33.4% to 51.2%. In this case, the removal rates of xylan and lignin reache up to 86.7% and 39.0%, respectively. After 72 h enzymatic hydrolysis by cellulase, the productions of glucose and fermentable sugars are relatively high, reaching 29.0 g and 30.7 g from 100 g of corn stover. Therefore, the H2SO4-assisted DES system efficiently remove xylan and lignin, which facilitates the improvements of hydrolysis and fermentable sugars production. This study develops a green and high-efficient pretreatment to provide fundamental data and technical support for valorization of renewable and abundant agricultural residues in China.
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institution Kabale University
issn 1008-1542
language zho
publishDate 2025-06-01
publisher Hebei University of Science and Technology
record_format Article
series Journal of Hebei University of Science and Technology
spelling doaj-art-335351e62ec04ca29941fcf925d845d42025-08-20T03:29:09ZzhoHebei University of Science and TechnologyJournal of Hebei University of Science and Technology1008-15422025-06-0146328829510.7535/hbkd.2025yx03006b202503006Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solventsJiabin WANG0Shijie WANG1Qianyue YANG2Rui ZHANG3Ruihong GAO4Rongling YANG5Hongzhen LUO6School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai′an, Jiangsu 223003, ChinaIn order to establish an efficient process for the pretreatment of lignocellulosic biomass to deconstruct the complex structure and improve enzymatic hydrolysis efficiency of polysaccharide fractions, choline chloride(ChCl) and glycerol(Gly) were used to synthesize green deep eutectic solvent(DES) for corn stover pretreatment.The deconstruction effects of DES on corn stover under different synthesis conditions were compared, and the chemical reagents were added to enhance pretreatment. The results indicate that, pretreatment at 120 ℃ for 3 h by DES (at a molar ratio of 1∶2) with 1% H2SO4 addition improves the glucan content from 33.4% to 51.2%. In this case, the removal rates of xylan and lignin reache up to 86.7% and 39.0%, respectively. After 72 h enzymatic hydrolysis by cellulase, the productions of glucose and fermentable sugars are relatively high, reaching 29.0 g and 30.7 g from 100 g of corn stover. Therefore, the H2SO4-assisted DES system efficiently remove xylan and lignin, which facilitates the improvements of hydrolysis and fermentable sugars production. This study develops a green and high-efficient pretreatment to provide fundamental data and technical support for valorization of renewable and abundant agricultural residues in China.https://xuebao.hebust.edu.cn/hbkjdx/article/pdf/b202503006?st=article_issuebiochemical engineering; agricultural residue; pretreatment; deep eutectic solvent; enzymatic hydrolysis; fermentable sugar
spellingShingle Jiabin WANG
Shijie WANG
Qianyue YANG
Rui ZHANG
Ruihong GAO
Rongling YANG
Hongzhen LUO
Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents
Journal of Hebei University of Science and Technology
biochemical engineering; agricultural residue; pretreatment; deep eutectic solvent; enzymatic hydrolysis; fermentable sugar
title Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents
title_full Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents
title_fullStr Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents
title_full_unstemmed Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents
title_short Research on fractionation of corn stover to produce fermentable sugar by using choline chloride-based deep eutectic solvents
title_sort research on fractionation of corn stover to produce fermentable sugar by using choline chloride based deep eutectic solvents
topic biochemical engineering; agricultural residue; pretreatment; deep eutectic solvent; enzymatic hydrolysis; fermentable sugar
url https://xuebao.hebust.edu.cn/hbkjdx/article/pdf/b202503006?st=article_issue
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