Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer
As a member of helix–loop–helix protein family, transcription factor 12 functions as either an oncogene or a tumor suppressor in various human cancers. However, there are no reports on its involvement in prostate cancer. To investigate clinical relevance of transcription factor 12 in prostate cancer...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-06-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317703924 |
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| author | Qing-biao Chen Ying-ke Liang Yan-qiong Zhang Min-yao Jiang Zhao-dong Han Yu-xiang Liang Yue-ping Wan Jie Yin Hui-Chan He Wei-de Zhong |
| author_facet | Qing-biao Chen Ying-ke Liang Yan-qiong Zhang Min-yao Jiang Zhao-dong Han Yu-xiang Liang Yue-ping Wan Jie Yin Hui-Chan He Wei-de Zhong |
| author_sort | Qing-biao Chen |
| collection | DOAJ |
| description | As a member of helix–loop–helix protein family, transcription factor 12 functions as either an oncogene or a tumor suppressor in various human cancers. However, there are no reports on its involvement in prostate cancer. To investigate clinical relevance of transcription factor 12 in prostate cancer and to evaluate its roles in malignant phenotypes of this cancer in vitro and in vivo, we here examined expression patterns of transcription factor 12 protein in 50 prostate cancer tissue specimens by immunohistochemistry. Then, associations of transcription factor 12 expression with various clinicopathological characteristics and patients’ prognosis of prostate cancer were evaluated. Its involvements in cancer cell proliferation, migration, invasion, and tumor growth were determined by in vitro and in vivo experiments. As a result, the positive immunostaining of transcription factor 12 protein was localized in cytoplasm and/or nucleus of prostate cancer cells. Its expression levels were decreased with prostate cancer Gleason score increased. Statistically, the decreased expression of transcription factor 12 protein more frequently occurred in prostate cancer patients with high Gleason score, positive metastasis, prostate-specific antigen failure, and short biochemical recurrence–free survival (all p < 0.05). Importantly, multivariate analysis showed that the status of transcription factor 12 expression was an independent predictor of biochemical recurrence–free survival in prostate cancer. Functionally, enforced expression of transcription factor 12 suppressed cell proliferation, migration, and invasion in vitro and inhibited tumor growth in vivo. In conclusion, transcription factor 12 protein may be a novel molecule which plays a critical role in prostate cancer progression and patients’ prognosis, suggesting it might be a promising therapeutic target for prostate cancer therapy. |
| format | Article |
| id | doaj-art-3338c7bd8b9349b8a09aef6c8d6406fb |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-3338c7bd8b9349b8a09aef6c8d6406fb2025-08-20T03:28:37ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317703924Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancerQing-biao Chen0Ying-ke Liang1Yan-qiong Zhang2Min-yao Jiang3Zhao-dong Han4Yu-xiang Liang5Yue-ping Wan6Jie Yin7Hui-Chan He8Wei-de Zhong9Department of Urology, Huadu District People’s Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, ChinaChina Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaDepartment of Urology, Huadu District People’s Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, ChinaDepartment of Urology, Huadu District People’s Hospital, Southern Medical University, Guangzhou, ChinaAffiliated Foshan Hospital of Southern Medical University, Southern Medical University, Foshan, ChinaUrology Key Laboratory of Guangdong Province, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaUrology Key Laboratory of Guangdong Province, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, ChinaAs a member of helix–loop–helix protein family, transcription factor 12 functions as either an oncogene or a tumor suppressor in various human cancers. However, there are no reports on its involvement in prostate cancer. To investigate clinical relevance of transcription factor 12 in prostate cancer and to evaluate its roles in malignant phenotypes of this cancer in vitro and in vivo, we here examined expression patterns of transcription factor 12 protein in 50 prostate cancer tissue specimens by immunohistochemistry. Then, associations of transcription factor 12 expression with various clinicopathological characteristics and patients’ prognosis of prostate cancer were evaluated. Its involvements in cancer cell proliferation, migration, invasion, and tumor growth were determined by in vitro and in vivo experiments. As a result, the positive immunostaining of transcription factor 12 protein was localized in cytoplasm and/or nucleus of prostate cancer cells. Its expression levels were decreased with prostate cancer Gleason score increased. Statistically, the decreased expression of transcription factor 12 protein more frequently occurred in prostate cancer patients with high Gleason score, positive metastasis, prostate-specific antigen failure, and short biochemical recurrence–free survival (all p < 0.05). Importantly, multivariate analysis showed that the status of transcription factor 12 expression was an independent predictor of biochemical recurrence–free survival in prostate cancer. Functionally, enforced expression of transcription factor 12 suppressed cell proliferation, migration, and invasion in vitro and inhibited tumor growth in vivo. In conclusion, transcription factor 12 protein may be a novel molecule which plays a critical role in prostate cancer progression and patients’ prognosis, suggesting it might be a promising therapeutic target for prostate cancer therapy.https://doi.org/10.1177/1010428317703924 |
| spellingShingle | Qing-biao Chen Ying-ke Liang Yan-qiong Zhang Min-yao Jiang Zhao-dong Han Yu-xiang Liang Yue-ping Wan Jie Yin Hui-Chan He Wei-de Zhong Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer Tumor Biology |
| title | Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer |
| title_full | Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer |
| title_fullStr | Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer |
| title_full_unstemmed | Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer |
| title_short | Decreased expression of TCF12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer |
| title_sort | decreased expression of tcf12 contributes to progression and predicts biochemical recurrence in patients with prostate cancer |
| url | https://doi.org/10.1177/1010428317703924 |
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