Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae
Background/Objectives: Epilepsy is a common neurological condition characterized by the occurrence of a seizure. It affects around 50 million individuals worldwide, and despite the large quantity of anti-seizure medications available, 30% of epileptic patients still suffer from seizures. Therefore,...
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MDPI AG
2025-05-01
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| Series: | Future Pharmacology |
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| Online Access: | https://www.mdpi.com/2673-9879/5/2/22 |
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| author | Fernanda Barros de Miranda Lucia Emanueli Schimith Dennis Guilherme da Costa Silva Camila de Oliveira Vian Diele Bopsin da Luz Rafael Felipe de Aguiar Crístian Yan Montana da Rocha Anna Maria Siebel Jean Pierre Oses Mariana Appel Hort |
| author_facet | Fernanda Barros de Miranda Lucia Emanueli Schimith Dennis Guilherme da Costa Silva Camila de Oliveira Vian Diele Bopsin da Luz Rafael Felipe de Aguiar Crístian Yan Montana da Rocha Anna Maria Siebel Jean Pierre Oses Mariana Appel Hort |
| author_sort | Fernanda Barros de Miranda |
| collection | DOAJ |
| description | Background/Objectives: Epilepsy is a common neurological condition characterized by the occurrence of a seizure. It affects around 50 million individuals worldwide, and despite the large quantity of anti-seizure medications available, 30% of epileptic patients still suffer from seizures. Therefore, it is necessary to find new therapeutic options. Interestingly, polydatin has shown promising effects on epilepsy treatment due to its antioxidant and anti-inflammatory properties. Thus, this study aimed to evaluate the effects of polydatin (200, 300, and 400 µM) on a pentylenetetrazol (PTZ)-induced seizure model in wild-type zebrafish (<i>Danio rerio</i>) larvae. Methods: Seizure-like behavior, cell death, reactive species (RS) production, and lipid peroxidation were analyzed. Results: Pre-treatment with polydatin at 200 and 300 µM did not have a significant impact on seizure occurrence and the behavior of animals exposed to PTZ. Diazepam decreased seizure occurrence and increased the latency to achieve each seizure stage. Exposure to PTZ increased the swimming activity, and this effect was suppressed by diazepam but not by polydatin. PTZ exposure increased the RS production, which was significantly attenuated by polydatin at 400 µM and DMSO. Cell death and lipid peroxidation were not changed when compared to the experimental groups. Conclusions: Only the experimental positive control (diazepam) showed anti-seizure effects. Therefore, we failed to observe any anti-seizure effects of polydatin using a zebrafish experimental model. However, we cannot rule out its effects in other experimental models and different treatment protocols. |
| format | Article |
| id | doaj-art-332fedf5f8f94acd93605bfb100232a3 |
| institution | Kabale University |
| issn | 2673-9879 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Future Pharmacology |
| spelling | doaj-art-332fedf5f8f94acd93605bfb100232a32025-08-20T03:26:52ZengMDPI AGFuture Pharmacology2673-98792025-05-01522210.3390/futurepharmacol5020022Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish LarvaeFernanda Barros de Miranda0Lucia Emanueli Schimith1Dennis Guilherme da Costa Silva2Camila de Oliveira Vian3Diele Bopsin da Luz4Rafael Felipe de Aguiar5Crístian Yan Montana da Rocha6Anna Maria Siebel7Jean Pierre Oses8Mariana Appel Hort9Postgraduate Program in Health Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Health Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Health Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Health Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Physiological Sciences, Institute of Biological Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilPostgraduate Program in Health Sciences, Federal University of Rio Grande, FURG, Rio Grande 96203-900, RS, BrazilBackground/Objectives: Epilepsy is a common neurological condition characterized by the occurrence of a seizure. It affects around 50 million individuals worldwide, and despite the large quantity of anti-seizure medications available, 30% of epileptic patients still suffer from seizures. Therefore, it is necessary to find new therapeutic options. Interestingly, polydatin has shown promising effects on epilepsy treatment due to its antioxidant and anti-inflammatory properties. Thus, this study aimed to evaluate the effects of polydatin (200, 300, and 400 µM) on a pentylenetetrazol (PTZ)-induced seizure model in wild-type zebrafish (<i>Danio rerio</i>) larvae. Methods: Seizure-like behavior, cell death, reactive species (RS) production, and lipid peroxidation were analyzed. Results: Pre-treatment with polydatin at 200 and 300 µM did not have a significant impact on seizure occurrence and the behavior of animals exposed to PTZ. Diazepam decreased seizure occurrence and increased the latency to achieve each seizure stage. Exposure to PTZ increased the swimming activity, and this effect was suppressed by diazepam but not by polydatin. PTZ exposure increased the RS production, which was significantly attenuated by polydatin at 400 µM and DMSO. Cell death and lipid peroxidation were not changed when compared to the experimental groups. Conclusions: Only the experimental positive control (diazepam) showed anti-seizure effects. Therefore, we failed to observe any anti-seizure effects of polydatin using a zebrafish experimental model. However, we cannot rule out its effects in other experimental models and different treatment protocols.https://www.mdpi.com/2673-9879/5/2/22anti-seizure<i>Danio rerio</i>epilepsypolydatinseizureszebrafish |
| spellingShingle | Fernanda Barros de Miranda Lucia Emanueli Schimith Dennis Guilherme da Costa Silva Camila de Oliveira Vian Diele Bopsin da Luz Rafael Felipe de Aguiar Crístian Yan Montana da Rocha Anna Maria Siebel Jean Pierre Oses Mariana Appel Hort Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae Future Pharmacology anti-seizure <i>Danio rerio</i> epilepsy polydatin seizures zebrafish |
| title | Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae |
| title_full | Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae |
| title_fullStr | Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae |
| title_full_unstemmed | Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae |
| title_short | Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae |
| title_sort | effects of polydatin on pentylenetetrazol induced seizures in zebrafish larvae |
| topic | anti-seizure <i>Danio rerio</i> epilepsy polydatin seizures zebrafish |
| url | https://www.mdpi.com/2673-9879/5/2/22 |
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