Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje
Background/Aim. Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains appear to have rapidly disseminated among population in the community without established risk factors for MRSA worldwide. Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by th...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2016-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501600003P.pdf |
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| Summary: | Background/Aim. Community-associated methicillin-resistant Staphylococcus
aureus (CA-MRSA) strains appear to have rapidly disseminated among population
in the community without established risk factors for MRSA worldwide.
Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by the
lukF-PV and lukF-PV genes. PVL may be the key toxin responsible for enhanced
virulence of CA-MRSA. The aim of this study was to detect the genes encoding
PVL in CA-MRSA isolates from healthy people from the District of Pomoravlje.
Methods. We took throat and nose swabs from healthy, employed persons with
mandatory sanitary examinations and analyzed the presence of MRSA, between
January 2011 and December 2012 in the District of Pomoravlje. Susceptibility
of isolated strains to cefoxitin was investigated by using disc diffusion
according to the recommendation of CLSI (Clinical Laboratory Standard
Institute), and by E test. The presence of penicillin-binding protein 2a
(PBP2a) in Staphylococci was detected using latex agglutination Slidex ®MRSA
Detection test. The gold standard, polymerase chain reaction (PCR) assay, was
used for detection of mecA gene and PVL gene, and typing of SCCmec region.
Results. Our investigation showed that staphylococcal carrier state was
present in 2.58% of 52,910 throat and nasal swabs, and in 50 of them (3.67%)
MRSA was isolated. Among these MRSA, 2 (4%) isolates were PVL-positive.
Conclusion. The prevalence of CAMRSA and the presence of PVL gene among
healthy, employed population in the District of Pomoravlje were low. The
values obtained in this study show that, our region is not significantly
different from the other parts of our country, nor from the other European
countries. |
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| ISSN: | 0042-8450 2406-0720 |