Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers.
<h4>Introduction</h4>Despite implementation of the biological passport to detect erythropoietin abuse, a need for additional biomarkers remains. We used a proteomic approach to identify novel serum biomarkers of prolonged erythropoiesis-stimulating agent (ESA) exposure (Darbepoietin-α) a...
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117119&type=printable |
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| author | Britt Christensen Maja Ludvigsen Birgitte Nellemann John J Kopchick Bent Honoré Jens Otto L Jørgensen |
| author_facet | Britt Christensen Maja Ludvigsen Birgitte Nellemann John J Kopchick Bent Honoré Jens Otto L Jørgensen |
| author_sort | Britt Christensen |
| collection | DOAJ |
| description | <h4>Introduction</h4>Despite implementation of the biological passport to detect erythropoietin abuse, a need for additional biomarkers remains. We used a proteomic approach to identify novel serum biomarkers of prolonged erythropoiesis-stimulating agent (ESA) exposure (Darbepoietin-α) and/or aerobic training.<h4>Trial design</h4>Thirty-six healthy young males were randomly assigned to the following groups: Sedentary-placebo (n = 9), Sedentary-ESA (n = 9), Training-placebo (n = 10), or Training-ESA (n = 8). They were treated with placebo/Darbepoietin-α subcutaneously once/week for 10 weeks followed by a 3-week washout period. Training consisted of supervised biking 3/week for 13 weeks at the highest possible intensity. Serum was collected at baseline, week 3 (high dose Darbepoietin-α), week 10 (reduced dose Darbepoietin-α), and after a 3-week washout period.<h4>Methods</h4>Serum proteins were separated according to charge and molecular mass (2D-gel electrophoresis). The identity of proteins from spots exhibiting altered intensity was determined by mass spectrometry.<h4>Results</h4>Six protein spots changed in response to Darbepoietin-α treatment. Comparing all 4 experimental groups, two protein spots (serotransferrin and haptoglobin/haptoglobin related protein) showed a significant response to Darbepoietin-α treatment. The haptoglobin/haptoglobin related protein spot showed a significantly lower intensity in all subjects in the training-ESA group during the treatment period and increased during the washout period.<h4>Conclusion</h4>An isoform of haptoglobin/haptoglobin related protein could be a new anti-doping marker and merits further research.<h4>Trial registration</h4>ClinicalTrials.gov NCT01320449. |
| format | Article |
| id | doaj-art-332819133b804a729722c8f4ae91ad2d |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-332819133b804a729722c8f4ae91ad2d2025-08-20T02:20:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011711910.1371/journal.pone.0117119Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers.Britt ChristensenMaja LudvigsenBirgitte NellemannJohn J KopchickBent HonoréJens Otto L Jørgensen<h4>Introduction</h4>Despite implementation of the biological passport to detect erythropoietin abuse, a need for additional biomarkers remains. We used a proteomic approach to identify novel serum biomarkers of prolonged erythropoiesis-stimulating agent (ESA) exposure (Darbepoietin-α) and/or aerobic training.<h4>Trial design</h4>Thirty-six healthy young males were randomly assigned to the following groups: Sedentary-placebo (n = 9), Sedentary-ESA (n = 9), Training-placebo (n = 10), or Training-ESA (n = 8). They were treated with placebo/Darbepoietin-α subcutaneously once/week for 10 weeks followed by a 3-week washout period. Training consisted of supervised biking 3/week for 13 weeks at the highest possible intensity. Serum was collected at baseline, week 3 (high dose Darbepoietin-α), week 10 (reduced dose Darbepoietin-α), and after a 3-week washout period.<h4>Methods</h4>Serum proteins were separated according to charge and molecular mass (2D-gel electrophoresis). The identity of proteins from spots exhibiting altered intensity was determined by mass spectrometry.<h4>Results</h4>Six protein spots changed in response to Darbepoietin-α treatment. Comparing all 4 experimental groups, two protein spots (serotransferrin and haptoglobin/haptoglobin related protein) showed a significant response to Darbepoietin-α treatment. The haptoglobin/haptoglobin related protein spot showed a significantly lower intensity in all subjects in the training-ESA group during the treatment period and increased during the washout period.<h4>Conclusion</h4>An isoform of haptoglobin/haptoglobin related protein could be a new anti-doping marker and merits further research.<h4>Trial registration</h4>ClinicalTrials.gov NCT01320449.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117119&type=printable |
| spellingShingle | Britt Christensen Maja Ludvigsen Birgitte Nellemann John J Kopchick Bent Honoré Jens Otto L Jørgensen Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers. PLoS ONE |
| title | Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers. |
| title_full | Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers. |
| title_fullStr | Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers. |
| title_full_unstemmed | Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers. |
| title_short | Serum proteomic changes after randomized prolonged erythropoietin treatment and/or endurance training: detection of novel biomarkers. |
| title_sort | serum proteomic changes after randomized prolonged erythropoietin treatment and or endurance training detection of novel biomarkers |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117119&type=printable |
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