Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China
Pokkah Boeng Disease (PBD) is a severe and devastating disease that causes significant damage and yield losses in China. The pathogenic fungus <i>Fusarium oxysporum</i> is responsible for the rapid onset of top rot symptoms in sugarcane. In this study, we selected a representative strain...
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2025-03-01
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| author | Wenfeng Lin Chi Zhang Sehrish Akbar Suyan Wu Yabing Yue Gege Wang Yu Zhou Charles A. Powell Wei Yao Jianlong Xu Baoshan Chen Muqing Zhang Yixue Bao |
| author_facet | Wenfeng Lin Chi Zhang Sehrish Akbar Suyan Wu Yabing Yue Gege Wang Yu Zhou Charles A. Powell Wei Yao Jianlong Xu Baoshan Chen Muqing Zhang Yixue Bao |
| author_sort | Wenfeng Lin |
| collection | DOAJ |
| description | Pokkah Boeng Disease (PBD) is a severe and devastating disease that causes significant damage and yield losses in China. The pathogenic fungus <i>Fusarium oxysporum</i> is responsible for the rapid onset of top rot symptoms in sugarcane. In this study, we selected a representative strain, BS2-6, to perform morphological observations of colonies and determine pathogenicity. We examined the effects of BS2-6 infestation on the ultrastructure of sugarcane leaves. Moreover, we sequenced the whole genome of BS2-6 and examined the effects of various nitrogen sources and chemical reagents on its growth and pathogenicity. Our results indicate that sugarcane leaves inoculated with BS2-6 quickly succumb to heart leaf and growing rot. Ultrastructural analysis revealed that the surface tissues of the diseased leaves were destroyed with mycelium, and conidia blocked leaf stomata, which ultimately led to the degradation of leaf tissues. Ammoniacal nitrogen significantly promoted mycelial growth, pigment secretion, and the expression of genes related to secondary metabolite synthesis, thereby accelerating the development of PBD. In addition, we found that carbendazim effectively inhibited the growth of BS2-6 at various concentrations. These findings provide important insights for the effective prevention and control of PBD during sugarcane production. |
| format | Article |
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| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-33233cd49a2c4e8aa8cfcde03a633a752025-08-20T01:48:53ZengMDPI AGMicroorganisms2076-26072025-03-0113357310.3390/microorganisms13030573Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in ChinaWenfeng Lin0Chi Zhang1Sehrish Akbar2Suyan Wu3Yabing Yue4Gege Wang5Yu Zhou6Charles A. Powell7Wei Yao8Jianlong Xu9Baoshan Chen10Muqing Zhang11Yixue Bao12State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaIndian River Research and Education Center-Institute of Food and Agricultura, University of Florida, Fort Pierce, FL 34945, USAState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaState Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology, Guangxi University, Nanning 530004, ChinaPokkah Boeng Disease (PBD) is a severe and devastating disease that causes significant damage and yield losses in China. The pathogenic fungus <i>Fusarium oxysporum</i> is responsible for the rapid onset of top rot symptoms in sugarcane. In this study, we selected a representative strain, BS2-6, to perform morphological observations of colonies and determine pathogenicity. We examined the effects of BS2-6 infestation on the ultrastructure of sugarcane leaves. Moreover, we sequenced the whole genome of BS2-6 and examined the effects of various nitrogen sources and chemical reagents on its growth and pathogenicity. Our results indicate that sugarcane leaves inoculated with BS2-6 quickly succumb to heart leaf and growing rot. Ultrastructural analysis revealed that the surface tissues of the diseased leaves were destroyed with mycelium, and conidia blocked leaf stomata, which ultimately led to the degradation of leaf tissues. Ammoniacal nitrogen significantly promoted mycelial growth, pigment secretion, and the expression of genes related to secondary metabolite synthesis, thereby accelerating the development of PBD. In addition, we found that carbendazim effectively inhibited the growth of BS2-6 at various concentrations. These findings provide important insights for the effective prevention and control of PBD during sugarcane production.https://www.mdpi.com/2076-2607/13/3/573<i>Fusarium oxysporum</i>Pokkah Boeng diseasepathogenic mechanismdisease prevention and control |
| spellingShingle | Wenfeng Lin Chi Zhang Sehrish Akbar Suyan Wu Yabing Yue Gege Wang Yu Zhou Charles A. Powell Wei Yao Jianlong Xu Baoshan Chen Muqing Zhang Yixue Bao Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China Microorganisms <i>Fusarium oxysporum</i> Pokkah Boeng disease pathogenic mechanism disease prevention and control |
| title | Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China |
| title_full | Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China |
| title_fullStr | Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China |
| title_full_unstemmed | Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China |
| title_short | Characterization and Genome Analysis of <i>Fusarium oxysporum</i> Provides Insights into the Pathogenic Mechanisms of the Pokkah Boeng Disease in China |
| title_sort | characterization and genome analysis of i fusarium oxysporum i provides insights into the pathogenic mechanisms of the pokkah boeng disease in china |
| topic | <i>Fusarium oxysporum</i> Pokkah Boeng disease pathogenic mechanism disease prevention and control |
| url | https://www.mdpi.com/2076-2607/13/3/573 |
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