Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model
Abstract Antimicrobial peptides (AMPs) are instrumental in maintaining intestinal homeostasis and have emerged as potential therapeutic candidates for ameliorating intestinal bacterial infections. However, the intrinsic instability associated with the in vivo delivery of AMPs constitutes a substanti...
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BMC
2025-02-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-024-01438-z |
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| author | Wei Li Xue Wang Keyuan Chen Yaohong Zhu Guiyan Yang Yipeng Jin Jiufeng Wang |
| author_facet | Wei Li Xue Wang Keyuan Chen Yaohong Zhu Guiyan Yang Yipeng Jin Jiufeng Wang |
| author_sort | Wei Li |
| collection | DOAJ |
| description | Abstract Antimicrobial peptides (AMPs) are instrumental in maintaining intestinal homeostasis and have emerged as potential therapeutic candidates for ameliorating intestinal bacterial infections. However, the intrinsic instability associated with the in vivo delivery of AMPs constitutes a substantial impediment to their therapeutic efficacy in treating infections. In this study, we genetically modified Bacillus subtilis (B. subtilis) WB600 to express Zophobas atratus defensin (ZD), an antimicrobial peptide with broad-spectrum activity isolated from Zophobas atratus, for oral administration. This engineered strain effectively protects against Salmonella Infantis (S. Infantis) infection in mice. Pretreatment with WB600/ZD prevented NF-κB pathway activation induced by S. Infantis infection and increased expression of antioxidant and tight junction proteins, thus alleviating the severity of intestinal inflammation in both the jejunum and ileum (P < 0.01). Moreover, WB600/ZD pretreatment facilitated the growth of beneficial bacteria such as Lachnospiraceae, Butyricicoccus, Eubacterium_xylanophilum, and Clostridia_UCG-014 while decreasing the abundance of pathogenic bacteria such as Escherichia-Shigella and Salmonella (P < 0.05). In conclusion, this study underscores the protective effects of WB600/ZD on S. Infantis-induced intestinal inflammation, suggesting that oral delivery of B. subtilis WB600/ZD may be a promising prophylactic strategy for combating bacterial infections in the intestine. |
| format | Article |
| id | doaj-art-3322425a9ef64ebc88583bfe4bf01d08 |
| institution | Kabale University |
| issn | 1297-9716 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-3322425a9ef64ebc88583bfe4bf01d082025-02-09T12:50:03ZengBMCVeterinary Research1297-97162025-02-0156111610.1186/s13567-024-01438-zEngineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse modelWei Li0Xue Wang1Keyuan Chen2Yaohong Zhu3Guiyan Yang4Yipeng Jin5Jiufeng Wang6College of Veterinary Medicine, China Agricultural UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Veterinary Medicine, China Agricultural UniversityCollege of Veterinary Medicine, China Agricultural UniversityCollege of Veterinary Medicine, China Agricultural UniversityCollege of Veterinary Medicine, China Agricultural UniversityCollege of Veterinary Medicine, China Agricultural UniversityAbstract Antimicrobial peptides (AMPs) are instrumental in maintaining intestinal homeostasis and have emerged as potential therapeutic candidates for ameliorating intestinal bacterial infections. However, the intrinsic instability associated with the in vivo delivery of AMPs constitutes a substantial impediment to their therapeutic efficacy in treating infections. In this study, we genetically modified Bacillus subtilis (B. subtilis) WB600 to express Zophobas atratus defensin (ZD), an antimicrobial peptide with broad-spectrum activity isolated from Zophobas atratus, for oral administration. This engineered strain effectively protects against Salmonella Infantis (S. Infantis) infection in mice. Pretreatment with WB600/ZD prevented NF-κB pathway activation induced by S. Infantis infection and increased expression of antioxidant and tight junction proteins, thus alleviating the severity of intestinal inflammation in both the jejunum and ileum (P < 0.01). Moreover, WB600/ZD pretreatment facilitated the growth of beneficial bacteria such as Lachnospiraceae, Butyricicoccus, Eubacterium_xylanophilum, and Clostridia_UCG-014 while decreasing the abundance of pathogenic bacteria such as Escherichia-Shigella and Salmonella (P < 0.05). In conclusion, this study underscores the protective effects of WB600/ZD on S. Infantis-induced intestinal inflammation, suggesting that oral delivery of B. subtilis WB600/ZD may be a promising prophylactic strategy for combating bacterial infections in the intestine.https://doi.org/10.1186/s13567-024-01438-zZophobas atratus defensinengineered bacteriaB. subtilis WB600/ZDS. Infantis infectionsmall intestinal inflammation |
| spellingShingle | Wei Li Xue Wang Keyuan Chen Yaohong Zhu Guiyan Yang Yipeng Jin Jiufeng Wang Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model Veterinary Research Zophobas atratus defensin engineered bacteria B. subtilis WB600/ZD S. Infantis infection small intestinal inflammation |
| title | Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model |
| title_full | Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model |
| title_fullStr | Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model |
| title_full_unstemmed | Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model |
| title_short | Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model |
| title_sort | engineered bacillus subtilis wb600 zd prevents salmonella infantis induced intestinal inflammation and alters the colon microbiota in a mouse model |
| topic | Zophobas atratus defensin engineered bacteria B. subtilis WB600/ZD S. Infantis infection small intestinal inflammation |
| url | https://doi.org/10.1186/s13567-024-01438-z |
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