The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023
This study represents the first report on the detection and whole-genome sequencing of African swine fever (ASF) viruses in wild boar in Hong Kong in 2021–2023. Wild boar samples collected via an ASF surveillance program by the Agriculture, Fisheries, and Conservation Department were tested for ASF...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/17/7/896 |
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| author | Karina W. S. Tam Candy C. Y. Lau Timothy T. L. Ng Sin Ming Ip Sin Fat Pun Amanda Corla Carrie Batten Christopher J. Brackman |
| author_facet | Karina W. S. Tam Candy C. Y. Lau Timothy T. L. Ng Sin Ming Ip Sin Fat Pun Amanda Corla Carrie Batten Christopher J. Brackman |
| author_sort | Karina W. S. Tam |
| collection | DOAJ |
| description | This study represents the first report on the detection and whole-genome sequencing of African swine fever (ASF) viruses in wild boar in Hong Kong in 2021–2023. Wild boar samples collected via an ASF surveillance program by the Agriculture, Fisheries, and Conservation Department were tested for ASF viruses (ASFVs) using real-time polymerase chain reaction. ASF-positive carcasses were detected in four cases and hemadsorption, virus isolation, and whole-genome sequencing were conducted. The <i>B646L</i> gene, <i>E183L</i> gene, central variable region within the <i>B602L</i> gene, intergenic region between the <i>I73R</i> and <i>I329L</i> genes, <i>EP420R</i> gene, and multigene family members of the four ASFV strains were compared. The whole-genome phylogenetic relationships were studied. The comparative analysis of the genomes indicates that the ASFVs in these four cases have genetic similarities to Asian genotype II ASFVs, but are genetically distinct from each other, as well as the ASFV previously identified in a domestic pig farm in Hong Kong in 2021. |
| format | Article |
| id | doaj-art-331da51e89234a3dbc631ad34accc1c2 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-331da51e89234a3dbc631ad34accc1c22025-08-20T03:32:19ZengMDPI AGViruses1999-49152025-06-0117789610.3390/v17070896The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023Karina W. S. Tam0Candy C. Y. Lau1Timothy T. L. Ng2Sin Ming Ip3Sin Fat Pun4Amanda Corla5Carrie Batten6Christopher J. Brackman7Agriculture, Fisheries and Conservation Department, The Government of the Hong Kong Special Administrative Region, Hong Kong SAR, ChinaAgriculture, Fisheries and Conservation Department, The Government of the Hong Kong Special Administrative Region, Hong Kong SAR, ChinaAgriculture, Fisheries and Conservation Department, The Government of the Hong Kong Special Administrative Region, Hong Kong SAR, ChinaAgriculture, Fisheries and Conservation Department, The Government of the Hong Kong Special Administrative Region, Hong Kong SAR, ChinaAgriculture, Fisheries and Conservation Department, The Government of the Hong Kong Special Administrative Region, Hong Kong SAR, ChinaThe Pirbright Institute, Ash Road, Woking GU24 0NF, UKThe Pirbright Institute, Ash Road, Woking GU24 0NF, UKAgriculture, Fisheries and Conservation Department, The Government of the Hong Kong Special Administrative Region, Hong Kong SAR, ChinaThis study represents the first report on the detection and whole-genome sequencing of African swine fever (ASF) viruses in wild boar in Hong Kong in 2021–2023. Wild boar samples collected via an ASF surveillance program by the Agriculture, Fisheries, and Conservation Department were tested for ASF viruses (ASFVs) using real-time polymerase chain reaction. ASF-positive carcasses were detected in four cases and hemadsorption, virus isolation, and whole-genome sequencing were conducted. The <i>B646L</i> gene, <i>E183L</i> gene, central variable region within the <i>B602L</i> gene, intergenic region between the <i>I73R</i> and <i>I329L</i> genes, <i>EP420R</i> gene, and multigene family members of the four ASFV strains were compared. The whole-genome phylogenetic relationships were studied. The comparative analysis of the genomes indicates that the ASFVs in these four cases have genetic similarities to Asian genotype II ASFVs, but are genetically distinct from each other, as well as the ASFV previously identified in a domestic pig farm in Hong Kong in 2021.https://www.mdpi.com/1999-4915/17/7/896African swine feverAfrican swine fever virusASFVwild boarpigfull-length genome |
| spellingShingle | Karina W. S. Tam Candy C. Y. Lau Timothy T. L. Ng Sin Ming Ip Sin Fat Pun Amanda Corla Carrie Batten Christopher J. Brackman The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023 Viruses African swine fever African swine fever virus ASFV wild boar pig full-length genome |
| title | The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023 |
| title_full | The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023 |
| title_fullStr | The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023 |
| title_full_unstemmed | The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023 |
| title_short | The First African Swine Fever Viruses Detected in Wild Boar in Hong Kong, 2021–2023 |
| title_sort | first african swine fever viruses detected in wild boar in hong kong 2021 2023 |
| topic | African swine fever African swine fever virus ASFV wild boar pig full-length genome |
| url | https://www.mdpi.com/1999-4915/17/7/896 |
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