Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique.
<h4>Background</h4>Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and eva...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-06-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010138&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849337596594880512 |
|---|---|
| author | Rebeca Santano Rocío Rubio Berta Grau-Pujol Valdemiro Escola Osvaldo Muchisse Inocência Cuamba Marta Vidal Gemma Ruiz-Olalla Ruth Aguilar Javier Gandasegui Maria Demontis Jose Carlos Jamine Anélsio Cossa Charfudin Sacoor Jorge Cano Luis Izquierdo Chetan E Chitnis Ross L Coppel Virander Chauhan David Cavanagh Sheetij Dutta Evelina Angov Lisette van Lieshout Bin Zhan José Muñoz Carlota Dobaño Gemma Moncunill |
| author_facet | Rebeca Santano Rocío Rubio Berta Grau-Pujol Valdemiro Escola Osvaldo Muchisse Inocência Cuamba Marta Vidal Gemma Ruiz-Olalla Ruth Aguilar Javier Gandasegui Maria Demontis Jose Carlos Jamine Anélsio Cossa Charfudin Sacoor Jorge Cano Luis Izquierdo Chetan E Chitnis Ross L Coppel Virander Chauhan David Cavanagh Sheetij Dutta Evelina Angov Lisette van Lieshout Bin Zhan José Muñoz Carlota Dobaño Gemma Moncunill |
| author_sort | Rebeca Santano |
| collection | DOAJ |
| description | <h4>Background</h4>Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure.<h4>Methodology</h4>We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference.<h4>Principal findings</h4>IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed.<h4>Conclusions</h4>We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens. |
| format | Article |
| id | doaj-art-331b78039938433b9edbdb67c9d577ce |
| institution | Kabale University |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-331b78039938433b9edbdb67c9d577ce2025-08-20T03:44:39ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-06-01166e001013810.1371/journal.pntd.0010138Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique.Rebeca SantanoRocío RubioBerta Grau-PujolValdemiro EscolaOsvaldo MuchisseInocência CuambaMarta VidalGemma Ruiz-OlallaRuth AguilarJavier GandaseguiMaria DemontisJose Carlos JamineAnélsio CossaCharfudin SacoorJorge CanoLuis IzquierdoChetan E ChitnisRoss L CoppelVirander ChauhanDavid CavanaghSheetij DuttaEvelina AngovLisette van LieshoutBin ZhanJosé MuñozCarlota DobañoGemma Moncunill<h4>Background</h4>Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure.<h4>Methodology</h4>We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference.<h4>Principal findings</h4>IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed.<h4>Conclusions</h4>We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010138&type=printable |
| spellingShingle | Rebeca Santano Rocío Rubio Berta Grau-Pujol Valdemiro Escola Osvaldo Muchisse Inocência Cuamba Marta Vidal Gemma Ruiz-Olalla Ruth Aguilar Javier Gandasegui Maria Demontis Jose Carlos Jamine Anélsio Cossa Charfudin Sacoor Jorge Cano Luis Izquierdo Chetan E Chitnis Ross L Coppel Virander Chauhan David Cavanagh Sheetij Dutta Evelina Angov Lisette van Lieshout Bin Zhan José Muñoz Carlota Dobaño Gemma Moncunill Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique. PLoS Neglected Tropical Diseases |
| title | Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique. |
| title_full | Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique. |
| title_fullStr | Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique. |
| title_full_unstemmed | Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique. |
| title_short | Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique. |
| title_sort | evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co endemic area in southern mozambique |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010138&type=printable |
| work_keys_str_mv | AT rebecasantano evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT rociorubio evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT bertagraupujol evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT valdemiroescola evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT osvaldomuchisse evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT inocenciacuamba evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT martavidal evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT gemmaruizolalla evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT ruthaguilar evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT javiergandasegui evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT mariademontis evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT josecarlosjamine evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT anelsiocossa evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT charfudinsacoor evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT jorgecano evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT luisizquierdo evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT chetanechitnis evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT rosslcoppel evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT viranderchauhan evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT davidcavanagh evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT sheetijdutta evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT evelinaangov evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT lisettevanlieshout evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT binzhan evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT josemunoz evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT carlotadobano evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique AT gemmamoncunill evaluationofantibodyserologytodeterminecurrenthelminthandplasmodiumfalciparuminfectionsinacoendemicareainsouthernmozambique |