The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes
FTO expression correlates with adipose tissue development in rabbits, yet its regulatory role in preadipocyte differentiation remains poorly understood. Therefore, we employed transcriptome sequencing to identify 422 differentially expressed genes (DEGs) between the FTO overexpression group and the...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/13/1909 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849320010189635584 |
|---|---|
| author | Jiaxue Wang Wenqiang Sun Xianbo Jia Jie Wang Hengwei Yu Songjia Lai |
| author_facet | Jiaxue Wang Wenqiang Sun Xianbo Jia Jie Wang Hengwei Yu Songjia Lai |
| author_sort | Jiaxue Wang |
| collection | DOAJ |
| description | FTO expression correlates with adipose tissue development in rabbits, yet its regulatory role in preadipocyte differentiation remains poorly understood. Therefore, we employed transcriptome sequencing to identify 422 differentially expressed genes (DEGs) between the FTO overexpression group and the FTO-negative control group. Multi-omics evidence from proliferation assays (CCK-8), protein verification (WB), gene quantification (qPCR), and lipid staining (Oil Red O) established FTO as a positive regulator of rabbit preadipocyte development with gain-of-function enhancing and loss-of-function impeding differentiation. In conclusion, FTO regulates the proliferation and differentiation of rabbit preadipocytes, providing deeper insights into livestock energy metabolism and physiological functions. These findings hold significant implications for healthy animal farming, high-quality livestock product production, and the prevention and control of metabolic diseases in animals. |
| format | Article |
| id | doaj-art-3312b7dd4cd34dd2b71e84ca466ac9a0 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-3312b7dd4cd34dd2b71e84ca466ac9a02025-08-20T03:50:16ZengMDPI AGAnimals2076-26152025-06-011513190910.3390/ani15131909The Effects of FTO on the Proliferation and Differentiation of Rabbit PreadipocytesJiaxue Wang0Wenqiang Sun1Xianbo Jia2Jie Wang3Hengwei Yu4Songjia Lai5College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFTO expression correlates with adipose tissue development in rabbits, yet its regulatory role in preadipocyte differentiation remains poorly understood. Therefore, we employed transcriptome sequencing to identify 422 differentially expressed genes (DEGs) between the FTO overexpression group and the FTO-negative control group. Multi-omics evidence from proliferation assays (CCK-8), protein verification (WB), gene quantification (qPCR), and lipid staining (Oil Red O) established FTO as a positive regulator of rabbit preadipocyte development with gain-of-function enhancing and loss-of-function impeding differentiation. In conclusion, FTO regulates the proliferation and differentiation of rabbit preadipocytes, providing deeper insights into livestock energy metabolism and physiological functions. These findings hold significant implications for healthy animal farming, high-quality livestock product production, and the prevention and control of metabolic diseases in animals.https://www.mdpi.com/2076-2615/15/13/1909meat rabbitFTOpreadipocytecell proliferationdifferentiation |
| spellingShingle | Jiaxue Wang Wenqiang Sun Xianbo Jia Jie Wang Hengwei Yu Songjia Lai The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes Animals meat rabbit FTO preadipocyte cell proliferation differentiation |
| title | The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes |
| title_full | The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes |
| title_fullStr | The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes |
| title_full_unstemmed | The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes |
| title_short | The Effects of FTO on the Proliferation and Differentiation of Rabbit Preadipocytes |
| title_sort | effects of fto on the proliferation and differentiation of rabbit preadipocytes |
| topic | meat rabbit FTO preadipocyte cell proliferation differentiation |
| url | https://www.mdpi.com/2076-2615/15/13/1909 |
| work_keys_str_mv | AT jiaxuewang theeffectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT wenqiangsun theeffectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT xianbojia theeffectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT jiewang theeffectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT hengweiyu theeffectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT songjialai theeffectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT jiaxuewang effectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT wenqiangsun effectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT xianbojia effectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT jiewang effectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT hengweiyu effectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes AT songjialai effectsofftoontheproliferationanddifferentiationofrabbitpreadipocytes |