Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway

Esophageal squamous cell carcinoma (ESCC) is one of the most common forms of malignancy worldwide. However, there is currently a lack of effective chemotherapeutic drugs for ESCC. Ivermectin is a broad-spectrum antiparasitic drug with notable antitumor activity. However, the cellular and molecular m...

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Main Authors: Liu Huiyang, Chai Zhirong, Gao Ya, Wang Yanming, Lu Mengmeng
Format: Article
Language:English
Published: China Science Publishing & Media Ltd. 2024-11-01
Series:Acta Biochimica et Biophysica Sinica
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Online Access:https://www.sciengine.com/doi/10.3724/abbs.2024210
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author Liu Huiyang
Chai Zhirong
Gao Ya
Wang Yanming
Lu Mengmeng
author_facet Liu Huiyang
Chai Zhirong
Gao Ya
Wang Yanming
Lu Mengmeng
author_sort Liu Huiyang
collection DOAJ
description Esophageal squamous cell carcinoma (ESCC) is one of the most common forms of malignancy worldwide. However, there is currently a lack of effective chemotherapeutic drugs for ESCC. Ivermectin is a broad-spectrum antiparasitic drug with notable antitumor activity. However, the cellular and molecular mechanisms by which ivermectin inhibits cancer growth remain unclear. In this study, we elucidate the role of ivermectin in ESCC suppression by activating the endoplasmic reticulum (ER) stress and autophagy pathways. In transcriptome analyses, we find that activating transcription factor 4 (ATF4) and DNA damage inducible transcript 3 (DDIT3) are involved in the activation of ER stress by ivermectin. Moreover, ivermectin treatment suppresses the growth of ESCC xenograft tumors in nude mice. Taken together, our results establish the antitumor molecular role of ivermectin in targeting the ER stress-autophagy pathway and suggest that ivermectin is a potential drug candidate for the treatment of ESCC.
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institution OA Journals
issn 1672-9145
language English
publishDate 2024-11-01
publisher China Science Publishing & Media Ltd.
record_format Article
series Acta Biochimica et Biophysica Sinica
spelling doaj-art-3304b1ef6deb41eeaf07c1e7c51409752025-08-20T02:37:43ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452024-11-0157995100510.3724/abbs.202421020d259ccIvermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathwayLiu Huiyang0Chai Zhirong1Gao Ya2Wang Yanming3Lu Mengmeng4["Epigenetics & Translational Medicine Laboratory, School of Life Sciences, Henan University, Kaifeng 475004, China"]["Epigenetics & Translational Medicine Laboratory, School of Life Sciences, Henan University, Kaifeng 475004, China"]["Epigenetics & Translational Medicine Laboratory, School of Life Sciences, Henan University, Kaifeng 475004, China"]["The First Affiliated Hospital of Henan University, Henan University, Kaifeng 475004, China"]["Epigenetics & Translational Medicine Laboratory, School of Life Sciences, Henan University, Kaifeng 475004, China","State Key Laboratory of Antiviral Drugs, School of Pharmacy, Henan University, Kaifeng 475004, China"]Esophageal squamous cell carcinoma (ESCC) is one of the most common forms of malignancy worldwide. However, there is currently a lack of effective chemotherapeutic drugs for ESCC. Ivermectin is a broad-spectrum antiparasitic drug with notable antitumor activity. However, the cellular and molecular mechanisms by which ivermectin inhibits cancer growth remain unclear. In this study, we elucidate the role of ivermectin in ESCC suppression by activating the endoplasmic reticulum (ER) stress and autophagy pathways. In transcriptome analyses, we find that activating transcription factor 4 (ATF4) and DNA damage inducible transcript 3 (DDIT3) are involved in the activation of ER stress by ivermectin. Moreover, ivermectin treatment suppresses the growth of ESCC xenograft tumors in nude mice. Taken together, our results establish the antitumor molecular role of ivermectin in targeting the ER stress-autophagy pathway and suggest that ivermectin is a potential drug candidate for the treatment of ESCC.https://www.sciengine.com/doi/10.3724/abbs.2024210ivermectinESCCER stressATF4autophagy
spellingShingle Liu Huiyang
Chai Zhirong
Gao Ya
Wang Yanming
Lu Mengmeng
Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway
Acta Biochimica et Biophysica Sinica
ivermectin
ESCC
ER stress
ATF4
autophagy
title Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway
title_full Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway
title_fullStr Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway
title_full_unstemmed Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway
title_short Ivermectin inhibits the growth of ESCC by activating the ATF4-mediated endoplasmic reticulum stress-autophagy pathway
title_sort ivermectin inhibits the growth of escc by activating the atf4 mediated endoplasmic reticulum stress autophagy pathway
topic ivermectin
ESCC
ER stress
ATF4
autophagy
url https://www.sciengine.com/doi/10.3724/abbs.2024210
work_keys_str_mv AT liuhuiyang ivermectininhibitsthegrowthofesccbyactivatingtheatf4mediatedendoplasmicreticulumstressautophagypathway
AT chaizhirong ivermectininhibitsthegrowthofesccbyactivatingtheatf4mediatedendoplasmicreticulumstressautophagypathway
AT gaoya ivermectininhibitsthegrowthofesccbyactivatingtheatf4mediatedendoplasmicreticulumstressautophagypathway
AT wangyanming ivermectininhibitsthegrowthofesccbyactivatingtheatf4mediatedendoplasmicreticulumstressautophagypathway
AT lumengmeng ivermectininhibitsthegrowthofesccbyactivatingtheatf4mediatedendoplasmicreticulumstressautophagypathway