Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer

Abstract The interdisciplinary development of synthetic biology and material sciences propels medicine into a new era. For cancer therapy, living biotherapeutics integrating functional living bacteria with nanomedicine are particularly interesting. The current study developed a living biotherapeutic...

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Main Authors: Zhengwei Xu, Mingsong Zang, Hui Li, Ruizhen Tian, Zherui Zhang, Wang Liu, Fei Xiao, Xuesha Yan, Yan Zhu, Canhong Zhu, Jiayun Xu, Shuangjiang Yu, Tingting Wang, Hongcheng Sun, Junqiu Liu
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202502746
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author Zhengwei Xu
Mingsong Zang
Hui Li
Ruizhen Tian
Zherui Zhang
Wang Liu
Fei Xiao
Xuesha Yan
Yan Zhu
Canhong Zhu
Jiayun Xu
Shuangjiang Yu
Tingting Wang
Hongcheng Sun
Junqiu Liu
author_facet Zhengwei Xu
Mingsong Zang
Hui Li
Ruizhen Tian
Zherui Zhang
Wang Liu
Fei Xiao
Xuesha Yan
Yan Zhu
Canhong Zhu
Jiayun Xu
Shuangjiang Yu
Tingting Wang
Hongcheng Sun
Junqiu Liu
author_sort Zhengwei Xu
collection DOAJ
description Abstract The interdisciplinary development of synthetic biology and material sciences propels medicine into a new era. For cancer therapy, living biotherapeutics integrating functional living bacteria with nanomedicine are particularly interesting. The current study developed a living biotherapeutic platform integrating oxygen‐self‐supplying cyanobacteria with multifunctional prodrug nanoparticles to boost photodynamic immunotherapy. Generally, tetracarboxyl porphyrin is associated with cisplatin via a covalent self‐assembly strategy into uniform prodrug‐skeletal nanoparticles (ZnNCs). This helped encapsulate the antitumor drug dicumarol derivative (DicTBS). Later, these developed DicTBS‐ZnNC nanoparticles helped arm the surface of cyanobacteria using electrostatic adsorption to yield living nanotherapeutics (Cyano@DicTBS‐ZnNCs). Cyano@DicTBS‐ZnNCs achieved a self‐supply of nanoparticles and oxygen under 660 nm laser irradiation, producing PDT therapeutic effects. Furthermore, combining cisplatin and dicoumarol achieved synergistic anticancer effects. This approach also induced immunogenic cell death (ICD) and regulated the tumor microenvironment (TME). This promoted an immune‐supportive environment to improve antitumor immune responses.
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spelling doaj-art-32fb06dae7b849a48512e4cd64842a232025-08-20T02:40:00ZengWileyAdvanced Science2198-38442025-07-011227n/an/a10.1002/advs.202502746Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of CancerZhengwei Xu0Mingsong Zang1Hui Li2Ruizhen Tian3Zherui Zhang4Wang Liu5Fei Xiao6Xuesha Yan7Yan Zhu8Canhong Zhu9Jiayun Xu10Shuangjiang Yu11Tingting Wang12Hongcheng Sun13Junqiu Liu14College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaCollege of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education Hangzhou Normal University Hangzhou 311121 P. R. ChinaAbstract The interdisciplinary development of synthetic biology and material sciences propels medicine into a new era. For cancer therapy, living biotherapeutics integrating functional living bacteria with nanomedicine are particularly interesting. The current study developed a living biotherapeutic platform integrating oxygen‐self‐supplying cyanobacteria with multifunctional prodrug nanoparticles to boost photodynamic immunotherapy. Generally, tetracarboxyl porphyrin is associated with cisplatin via a covalent self‐assembly strategy into uniform prodrug‐skeletal nanoparticles (ZnNCs). This helped encapsulate the antitumor drug dicumarol derivative (DicTBS). Later, these developed DicTBS‐ZnNC nanoparticles helped arm the surface of cyanobacteria using electrostatic adsorption to yield living nanotherapeutics (Cyano@DicTBS‐ZnNCs). Cyano@DicTBS‐ZnNCs achieved a self‐supply of nanoparticles and oxygen under 660 nm laser irradiation, producing PDT therapeutic effects. Furthermore, combining cisplatin and dicoumarol achieved synergistic anticancer effects. This approach also induced immunogenic cell death (ICD) and regulated the tumor microenvironment (TME). This promoted an immune‐supportive environment to improve antitumor immune responses.https://doi.org/10.1002/advs.202502746cancer immunotherapycovalent self‐assemblyhypoxic tumorliving biotherapeuticsphotodynamic immunotherapy
spellingShingle Zhengwei Xu
Mingsong Zang
Hui Li
Ruizhen Tian
Zherui Zhang
Wang Liu
Fei Xiao
Xuesha Yan
Yan Zhu
Canhong Zhu
Jiayun Xu
Shuangjiang Yu
Tingting Wang
Hongcheng Sun
Junqiu Liu
Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer
Advanced Science
cancer immunotherapy
covalent self‐assembly
hypoxic tumor
living biotherapeutics
photodynamic immunotherapy
title Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer
title_full Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer
title_fullStr Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer
title_full_unstemmed Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer
title_short Living Biotherapeutics Using Nanoparticles‐Armed Cyanobacteria for Boosting Photodynamic‐Immunotherapy of Cancer
title_sort living biotherapeutics using nanoparticles armed cyanobacteria for boosting photodynamic immunotherapy of cancer
topic cancer immunotherapy
covalent self‐assembly
hypoxic tumor
living biotherapeutics
photodynamic immunotherapy
url https://doi.org/10.1002/advs.202502746
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