Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes
Autologous thrombocyte concentrate lysates, for example, platelet-released growth factors, (PRGFs) or their clinically related formulations (e.g., Vivostat PRF®) came recently into the physicians’ focus as they revealed promising effects in regenerative and reparative medicine such as the support of...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/5671615 |
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| _version_ | 1849387153174298624 |
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| author | Andreas Bayer Mersedeh Tohidnezhad Justus Lammel Sebastian Lippross Peter Behrendt Tim Klüter Thomas Pufe Holger Jahr Jochen Cremer Franziska Rademacher Regine Gläser Jürgen Harder |
| author_facet | Andreas Bayer Mersedeh Tohidnezhad Justus Lammel Sebastian Lippross Peter Behrendt Tim Klüter Thomas Pufe Holger Jahr Jochen Cremer Franziska Rademacher Regine Gläser Jürgen Harder |
| author_sort | Andreas Bayer |
| collection | DOAJ |
| description | Autologous thrombocyte concentrate lysates, for example, platelet-released growth factors, (PRGFs) or their clinically related formulations (e.g., Vivostat PRF®) came recently into the physicians’ focus as they revealed promising effects in regenerative and reparative medicine such as the support of healing of chronic wounds. To elucidate the underlying mechanisms, we analyzed the influence of PRGF and Vivostat PRF on human keratinocyte differentiation in vitro and on epidermal differentiation status of skin wounds in vivo. Therefore, we investigated the expression of early (keratin 1 and keratin 10) and late (transglutaminase-1 and involucrin) differentiation markers. PRGF treatment of primary human keratinocytes decreased keratin 1 and keratin 10 gene expression but induced involucrin and transglutaminase-1 gene expression in an epidermal growth factor receptor- (EGFR-) dependent manner. In concordance with these results, microscopic analyses revealed that PRGF-treated human keratinocytes displayed morphological features typical of keratinocytes undergoing terminal differentiation. In vivo treatment of artificial human wounds with Vivostat PRF revealed a significant induction of involucrin and transglutaminase-1 gene expression. Together, our results indicate that PRGF and Vivostat PRF induce terminal differentiation of primary human keratinocytes. This potential mechanism may contribute to the observed beneficial effects in the treatment of hard-to-heal wounds with autologous thrombocyte concentrate lysates in vivo. |
| format | Article |
| id | doaj-art-32f8eae91eae469bb2b21babc3aceaef |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-32f8eae91eae469bb2b21babc3aceaef2025-08-20T03:55:17ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/56716155671615Platelet-Released Growth Factors Induce Differentiation of Primary KeratinocytesAndreas Bayer0Mersedeh Tohidnezhad1Justus Lammel2Sebastian Lippross3Peter Behrendt4Tim Klüter5Thomas Pufe6Holger Jahr7Jochen Cremer8Franziska Rademacher9Regine Gläser10Jürgen Harder11Department of Heart and Vascular Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 18, 24105 Kiel, GermanyInstitute of Anatomy and Cell Biology, RWTH University of Aachen, Wendlingweg 2, 52072 Aachen, GermanyDepartment of Dermatology, University Hospital of Schleswig-Holstein, Campus Kiel, Rosalind-Franklin-Straße 7, 24105 Kiel, GermanyDepartment of Traumatology, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 18, 24105 Kiel, GermanyDepartment of Traumatology, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 18, 24105 Kiel, GermanyDepartment of Traumatology, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 18, 24105 Kiel, GermanyInstitute of Anatomy and Cell Biology, RWTH University of Aachen, Wendlingweg 2, 52072 Aachen, GermanyDepartment of Orthopedics, Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, GermanyDepartment of Heart and Vascular Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Arnold-Heller Straße 3, Haus 18, 24105 Kiel, GermanyDepartment of Dermatology, University Hospital of Schleswig-Holstein, Campus Kiel, Rosalind-Franklin-Straße 7, 24105 Kiel, GermanyDepartment of Dermatology, University Hospital of Schleswig-Holstein, Campus Kiel, Rosalind-Franklin-Straße 7, 24105 Kiel, GermanyDepartment of Dermatology, University Hospital of Schleswig-Holstein, Campus Kiel, Rosalind-Franklin-Straße 7, 24105 Kiel, GermanyAutologous thrombocyte concentrate lysates, for example, platelet-released growth factors, (PRGFs) or their clinically related formulations (e.g., Vivostat PRF®) came recently into the physicians’ focus as they revealed promising effects in regenerative and reparative medicine such as the support of healing of chronic wounds. To elucidate the underlying mechanisms, we analyzed the influence of PRGF and Vivostat PRF on human keratinocyte differentiation in vitro and on epidermal differentiation status of skin wounds in vivo. Therefore, we investigated the expression of early (keratin 1 and keratin 10) and late (transglutaminase-1 and involucrin) differentiation markers. PRGF treatment of primary human keratinocytes decreased keratin 1 and keratin 10 gene expression but induced involucrin and transglutaminase-1 gene expression in an epidermal growth factor receptor- (EGFR-) dependent manner. In concordance with these results, microscopic analyses revealed that PRGF-treated human keratinocytes displayed morphological features typical of keratinocytes undergoing terminal differentiation. In vivo treatment of artificial human wounds with Vivostat PRF revealed a significant induction of involucrin and transglutaminase-1 gene expression. Together, our results indicate that PRGF and Vivostat PRF induce terminal differentiation of primary human keratinocytes. This potential mechanism may contribute to the observed beneficial effects in the treatment of hard-to-heal wounds with autologous thrombocyte concentrate lysates in vivo.http://dx.doi.org/10.1155/2017/5671615 |
| spellingShingle | Andreas Bayer Mersedeh Tohidnezhad Justus Lammel Sebastian Lippross Peter Behrendt Tim Klüter Thomas Pufe Holger Jahr Jochen Cremer Franziska Rademacher Regine Gläser Jürgen Harder Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes Mediators of Inflammation |
| title | Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes |
| title_full | Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes |
| title_fullStr | Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes |
| title_full_unstemmed | Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes |
| title_short | Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes |
| title_sort | platelet released growth factors induce differentiation of primary keratinocytes |
| url | http://dx.doi.org/10.1155/2017/5671615 |
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