A mechanism regulating the onset of Sox2 expression in the embryonic neural plate.
In vertebrate embryos, the earliest definitive marker for the neural plate, which will give rise to the entire central nervous system, is the transcription factor Sox2. Although some of the extracellular signals that regulate neural plate fate have been identified, we know very little about the mech...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
|
| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060002&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849686722589229056 |
|---|---|
| author | Costis Papanayotou Anne Mey Anne-Marie Birot Yasushi Saka Sharon Boast Jim C Smith Jacques Samarut Claudio D Stern |
| author_facet | Costis Papanayotou Anne Mey Anne-Marie Birot Yasushi Saka Sharon Boast Jim C Smith Jacques Samarut Claudio D Stern |
| author_sort | Costis Papanayotou |
| collection | DOAJ |
| description | In vertebrate embryos, the earliest definitive marker for the neural plate, which will give rise to the entire central nervous system, is the transcription factor Sox2. Although some of the extracellular signals that regulate neural plate fate have been identified, we know very little about the mechanisms controlling Sox2 expression and thus neural plate identity. Here, we use electroporation for gain- and loss-of-function in the chick embryo, in combination with bimolecular fluorescence complementation, two-hybrid screens, chromatin immunoprecipitation, and reporter assays to study protein interactions that regulate expression of N2, the earliest enhancer of Sox2 to be activated and which directs expression to the largest part of the neural plate. We show that interactions between three coiled-coil domain proteins (ERNI, Geminin, and BERT), the heterochromatin proteins HP1alpha and HP1gamma acting as repressors, and the chromatin-remodeling enzyme Brm acting as activator control the N2 enhancer. We propose that this mechanism regulates the timing of Sox2 expression as part of the process of establishing neural plate identity. |
| format | Article |
| id | doaj-art-32f7ab3ffd2b4a5bad1fad64176a1616 |
| institution | DOAJ |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-32f7ab3ffd2b4a5bad1fad64176a16162025-08-20T03:22:35ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852008-01-0161e210.1371/journal.pbio.0060002A mechanism regulating the onset of Sox2 expression in the embryonic neural plate.Costis PapanayotouAnne MeyAnne-Marie BirotYasushi SakaSharon BoastJim C SmithJacques SamarutClaudio D SternIn vertebrate embryos, the earliest definitive marker for the neural plate, which will give rise to the entire central nervous system, is the transcription factor Sox2. Although some of the extracellular signals that regulate neural plate fate have been identified, we know very little about the mechanisms controlling Sox2 expression and thus neural plate identity. Here, we use electroporation for gain- and loss-of-function in the chick embryo, in combination with bimolecular fluorescence complementation, two-hybrid screens, chromatin immunoprecipitation, and reporter assays to study protein interactions that regulate expression of N2, the earliest enhancer of Sox2 to be activated and which directs expression to the largest part of the neural plate. We show that interactions between three coiled-coil domain proteins (ERNI, Geminin, and BERT), the heterochromatin proteins HP1alpha and HP1gamma acting as repressors, and the chromatin-remodeling enzyme Brm acting as activator control the N2 enhancer. We propose that this mechanism regulates the timing of Sox2 expression as part of the process of establishing neural plate identity.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060002&type=printable |
| spellingShingle | Costis Papanayotou Anne Mey Anne-Marie Birot Yasushi Saka Sharon Boast Jim C Smith Jacques Samarut Claudio D Stern A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. PLoS Biology |
| title | A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. |
| title_full | A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. |
| title_fullStr | A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. |
| title_full_unstemmed | A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. |
| title_short | A mechanism regulating the onset of Sox2 expression in the embryonic neural plate. |
| title_sort | mechanism regulating the onset of sox2 expression in the embryonic neural plate |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0060002&type=printable |
| work_keys_str_mv | AT costispapanayotou amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT annemey amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT annemariebirot amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT yasushisaka amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT sharonboast amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT jimcsmith amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT jacquessamarut amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT claudiodstern amechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT costispapanayotou mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT annemey mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT annemariebirot mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT yasushisaka mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT sharonboast mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT jimcsmith mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT jacquessamarut mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate AT claudiodstern mechanismregulatingtheonsetofsox2expressionintheembryonicneuralplate |