Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure
The effects of moderate hydrostatic pressure (MHP) pretreatment on bioactive metabolites in Tartary buckwheat sprouts remain insufficiently studied. In this work, a non-targeted metabolomics approach, combined with feature-based molecular networking (FBMN), identified 22 metabolites in sprouts treat...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015752500135X |
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| author | Liangyu Zhang Shuangyong Ding Rui Xu Li Xiao Jianxiong Chen Tao Wu Weili Li |
| author_facet | Liangyu Zhang Shuangyong Ding Rui Xu Li Xiao Jianxiong Chen Tao Wu Weili Li |
| author_sort | Liangyu Zhang |
| collection | DOAJ |
| description | The effects of moderate hydrostatic pressure (MHP) pretreatment on bioactive metabolites in Tartary buckwheat sprouts remain insufficiently studied. In this work, a non-targeted metabolomics approach, combined with feature-based molecular networking (FBMN), identified 22 metabolites in sprouts treated with 0–30 MPa pressure, four of which were previously unreported, thus expanding the species' phytochemical diversity. MHP treatment activated phenylalanine ammonia-lyase (PAL), resulting in a 2.3-fold increase in total flavonoids, particularly glycosylated derivatives such as quercetin 3-rutinoside 7-glucoside (13.2-fold increase at 25 MPa). In contrast, condensed tannins, responsible for astringency, were reduced by 40–60 %. These findings suggest that MHP pretreatment may serve as a sustainable alternative to chemical elicitors, promoting biofortification and improving sensory attributes in sprout-based functional foods. |
| format | Article |
| id | doaj-art-32f0f4cce2ff4dc7b81a6272b4efdcab |
| institution | DOAJ |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-32f0f4cce2ff4dc7b81a6272b4efdcab2025-08-20T02:55:29ZengElsevierFood Chemistry: X2590-15752025-02-012610228810.1016/j.fochx.2025.102288Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressureLiangyu Zhang0Shuangyong Ding1Rui Xu2Li Xiao3Jianxiong Chen4Tao Wu5Weili Li6Food Microbiology Key Laboratory of Sichuan Province, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Xihua University, No.999 Guangchang Road, Chengdu 610039, China.Food Microbiology Key Laboratory of Sichuan Province, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Xihua University, No.999 Guangchang Road, Chengdu 610039, China.College of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.Food Microbiology Key Laboratory of Sichuan Province, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Xihua University, No.999 Guangchang Road, Chengdu 610039, China.Sichuan Huan Tai Biotechnology Co., Ltd., 979 Konggang 3rd Road, Shuangliu District, Chengdu, Sichuan Province, Chengdu 610000, China.Food Microbiology Key Laboratory of Sichuan Province, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Xihua University, No.999 Guangchang Road, Chengdu 610039, China.; Corresponding authors:Food Microbiology Key Laboratory of Sichuan Province, Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Xihua University, No.999 Guangchang Road, Chengdu 610039, China.; Corresponding authors:The effects of moderate hydrostatic pressure (MHP) pretreatment on bioactive metabolites in Tartary buckwheat sprouts remain insufficiently studied. In this work, a non-targeted metabolomics approach, combined with feature-based molecular networking (FBMN), identified 22 metabolites in sprouts treated with 0–30 MPa pressure, four of which were previously unreported, thus expanding the species' phytochemical diversity. MHP treatment activated phenylalanine ammonia-lyase (PAL), resulting in a 2.3-fold increase in total flavonoids, particularly glycosylated derivatives such as quercetin 3-rutinoside 7-glucoside (13.2-fold increase at 25 MPa). In contrast, condensed tannins, responsible for astringency, were reduced by 40–60 %. These findings suggest that MHP pretreatment may serve as a sustainable alternative to chemical elicitors, promoting biofortification and improving sensory attributes in sprout-based functional foods.http://www.sciencedirect.com/science/article/pii/S259015752500135XFagopyrum tataricumGerminationFeature-based molecular networkModerate -hydrostatic pressureUHPLC-Q-TOF-MS/MS |
| spellingShingle | Liangyu Zhang Shuangyong Ding Rui Xu Li Xiao Jianxiong Chen Tao Wu Weili Li Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure Food Chemistry: X Fagopyrum tataricum Germination Feature-based molecular network Moderate -hydrostatic pressure UHPLC-Q-TOF-MS/MS |
| title | Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure |
| title_full | Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure |
| title_fullStr | Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure |
| title_full_unstemmed | Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure |
| title_short | Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure |
| title_sort | integrative metabolomics and molecular networking reveal metabolic rewiring in tartary buckwheat sprouts under moderate hydrostatic pressure |
| topic | Fagopyrum tataricum Germination Feature-based molecular network Moderate -hydrostatic pressure UHPLC-Q-TOF-MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S259015752500135X |
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