The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging
Background. Mesenchymal stem cells are the most commonly used seed cells in biomedical research and tissue engineering. Their secretory proteins have also been proven to play an important role in tissue healing. Methods. We isolated adipose stem cells and placental stem cells and performed analysis...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/7315830 |
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| author | Yan Xu Shilei Guo Cui Wei Honglan Li Lei Chen Chang Yin Chuansen Zhang |
| author_facet | Yan Xu Shilei Guo Cui Wei Honglan Li Lei Chen Chang Yin Chuansen Zhang |
| author_sort | Yan Xu |
| collection | DOAJ |
| description | Background. Mesenchymal stem cells are the most commonly used seed cells in biomedical research and tissue engineering. Their secretory proteins have also been proven to play an important role in tissue healing. Methods. We isolated adipose stem cells and placental stem cells and performed analysis examining characteristics. The secretory proteins were extracted from conditioned medium and analyzed by MALDI-TOF/TOF. The antiaging effect of conditioned mediums was evaluated by the results of facial skin application. Results. Adipose stem cells and placental stem cells were found to be very similar in their surface markers and multipotency. The specific proteins secreted from adipose stem cells were more adept at cell adhesion, migration, wound healing, and tissue remodeling, while the proteins secreted by placental stem cells were more adept at angiogenesis, cell proliferation, differentiation, cell survival, immunomodulation, and collagen degradation. While these two types of conditioned medium could improve the facial index, the improvement of Melanin index after injection of the adipose stem cell conditioned medium was much more significant. Conclusion. The results suggest that the secreted proteins are ideal cell-free substances for regeneration medicine, especially in the antiaging field. |
| format | Article |
| id | doaj-art-32effcbf62714834a2b42f6569d2e949 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-32effcbf62714834a2b42f6569d2e9492025-08-20T02:24:22ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/73158307315830The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial AntiagingYan Xu0Shilei Guo1Cui Wei2Honglan Li3Lei Chen4Chang Yin5Chuansen Zhang6Nanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, ChinaNanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, ChinaNanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, ChinaNanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, ChinaNanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, ChinaNanjing Regenerative Medicine Engineering and Technology Research Center, Nanjing 210046, ChinaRegenerative Medicine Center, The Second Military Medical University, Shanghai 200433, ChinaBackground. Mesenchymal stem cells are the most commonly used seed cells in biomedical research and tissue engineering. Their secretory proteins have also been proven to play an important role in tissue healing. Methods. We isolated adipose stem cells and placental stem cells and performed analysis examining characteristics. The secretory proteins were extracted from conditioned medium and analyzed by MALDI-TOF/TOF. The antiaging effect of conditioned mediums was evaluated by the results of facial skin application. Results. Adipose stem cells and placental stem cells were found to be very similar in their surface markers and multipotency. The specific proteins secreted from adipose stem cells were more adept at cell adhesion, migration, wound healing, and tissue remodeling, while the proteins secreted by placental stem cells were more adept at angiogenesis, cell proliferation, differentiation, cell survival, immunomodulation, and collagen degradation. While these two types of conditioned medium could improve the facial index, the improvement of Melanin index after injection of the adipose stem cell conditioned medium was much more significant. Conclusion. The results suggest that the secreted proteins are ideal cell-free substances for regeneration medicine, especially in the antiaging field.http://dx.doi.org/10.1155/2016/7315830 |
| spellingShingle | Yan Xu Shilei Guo Cui Wei Honglan Li Lei Chen Chang Yin Chuansen Zhang The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging Stem Cells International |
| title | The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging |
| title_full | The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging |
| title_fullStr | The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging |
| title_full_unstemmed | The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging |
| title_short | The Comparison of Adipose Stem Cell and Placental Stem Cell in Secretion Characteristics and in Facial Antiaging |
| title_sort | comparison of adipose stem cell and placental stem cell in secretion characteristics and in facial antiaging |
| url | http://dx.doi.org/10.1155/2016/7315830 |
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