Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats
Ethnopharmacological relevanceEucommia ulmoides, recognized as a traditional Chinese medicinal herb, can tonify liver and kidney and strengthen bones and muscles. Modern pharmacological research has proved that E. ulmoides could prohibit the occurrence of osteoporosis and arthritis.AimTo investigate...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1513863/full |
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| author | Baocang Yin Baocang Yin Mingzhen Yang Mingzhen Yang Bowen Wang Bowen Wang Yun Zhang Yun Zhang Ningli Li Ningli Li Qin Li Yingying Li Cory J. Xian Tiejun Li Tiejun Li Yuankun Zhai Yuankun Zhai |
| author_facet | Baocang Yin Baocang Yin Mingzhen Yang Mingzhen Yang Bowen Wang Bowen Wang Yun Zhang Yun Zhang Ningli Li Ningli Li Qin Li Yingying Li Cory J. Xian Tiejun Li Tiejun Li Yuankun Zhai Yuankun Zhai |
| author_sort | Baocang Yin |
| collection | DOAJ |
| description | Ethnopharmacological relevanceEucommia ulmoides, recognized as a traditional Chinese medicinal herb, can tonify liver and kidney and strengthen bones and muscles. Modern pharmacological research has proved that E. ulmoides could prohibit the occurrence of osteoporosis and arthritis.AimTo investigate the effect and action mechanism of total flavonoids isolated from the leaves of E. ulmoides (TFEL) on bone loss in ovariectomized (OVX) rats, and to study its effect on intestinal flora.Materials and methodsThe 3-month-old female rats were randomly divided into six groups: sham operation group, OVX model group, estradiol group, TFEL low (TFEL-L) (50), mid (-M) (100) and high (-H) (200 mg/kg/d) dose groups. After 13 weeks of treatment, the rats were sacrificed to measure bone turnover markers, related tissue biochemical indices, microstructure parameters, and osteoclastogenesis promotor RANKL and inhibitor OPG expression levels. Additionally, fecal samples were obtained for high-throughput sequencing to analyze the intestinal flora.ResultsOral administration of TFEL for 13 weeks increased the serum level of bone formation marker PINP and decreased the level of bone resorption marker NTX-I. The femoral microstructure parameters of the TFEL-M and TFEL-H groups were significantly improved compared with the OVX group, which were also confirmed by H&E histological staining. High-throughput sequencing indicated that TFEL may regulate the composition of intestinal flora and intestinal microecology.ConclusionTFEL can prevent osteoporosis in OVX rats and has no toxic side effects. Meanwhile, TFEL can increase the diversity and improve the composition of intestinal flora in OVX rats. |
| format | Article |
| id | doaj-art-32e7d388502d450d9db5b05ae24d7845 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-32e7d388502d450d9db5b05ae24d78452025-02-07T09:04:49ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-02-011610.3389/fphar.2025.15138631513863Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized ratsBaocang Yin0Baocang Yin1Mingzhen Yang2Mingzhen Yang3Bowen Wang4Bowen Wang5Yun Zhang6Yun Zhang7Ningli Li8Ningli Li9Qin Li10Yingying Li11Cory J. Xian12Tiejun Li13Tiejun Li14Yuankun Zhai15Yuankun Zhai16The First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaKaifeng Key Laboratory of Periodontal Tissue Engineering, Kaifeng, Henan, ChinaThe First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaKaifeng Key Laboratory of Periodontal Tissue Engineering, Kaifeng, Henan, ChinaThe First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaKaifeng Key Laboratory of Periodontal Tissue Engineering, Kaifeng, Henan, ChinaThe First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaSchool of Pharmacy, Henan University, Kaifeng, Henan, ChinaThe First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaKaifeng Key Laboratory of Periodontal Tissue Engineering, Kaifeng, Henan, ChinaSchool of Pharmacy, Henan University, Kaifeng, Henan, ChinaOsteoporosis Department, Luoyang Orthopedic-Traumatological Hospital, Luoyang, Henan, ChinaUniSA Clinical and Health Sciences, University of South Australia, Adelaide, SA, AustraliaThe First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaDepartment of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, ChinaThe First Affiliated Hospital of Henan University, Henan University School of Stomatology, Kaifeng, ChinaKaifeng Key Laboratory of Periodontal Tissue Engineering, Kaifeng, Henan, ChinaEthnopharmacological relevanceEucommia ulmoides, recognized as a traditional Chinese medicinal herb, can tonify liver and kidney and strengthen bones and muscles. Modern pharmacological research has proved that E. ulmoides could prohibit the occurrence of osteoporosis and arthritis.AimTo investigate the effect and action mechanism of total flavonoids isolated from the leaves of E. ulmoides (TFEL) on bone loss in ovariectomized (OVX) rats, and to study its effect on intestinal flora.Materials and methodsThe 3-month-old female rats were randomly divided into six groups: sham operation group, OVX model group, estradiol group, TFEL low (TFEL-L) (50), mid (-M) (100) and high (-H) (200 mg/kg/d) dose groups. After 13 weeks of treatment, the rats were sacrificed to measure bone turnover markers, related tissue biochemical indices, microstructure parameters, and osteoclastogenesis promotor RANKL and inhibitor OPG expression levels. Additionally, fecal samples were obtained for high-throughput sequencing to analyze the intestinal flora.ResultsOral administration of TFEL for 13 weeks increased the serum level of bone formation marker PINP and decreased the level of bone resorption marker NTX-I. The femoral microstructure parameters of the TFEL-M and TFEL-H groups were significantly improved compared with the OVX group, which were also confirmed by H&E histological staining. High-throughput sequencing indicated that TFEL may regulate the composition of intestinal flora and intestinal microecology.ConclusionTFEL can prevent osteoporosis in OVX rats and has no toxic side effects. Meanwhile, TFEL can increase the diversity and improve the composition of intestinal flora in OVX rats.https://www.frontiersin.org/articles/10.3389/fphar.2025.1513863/fullEucommia ulmoidestotal flavonoidsosteoporosisOvxintestinal microbiotauterus |
| spellingShingle | Baocang Yin Baocang Yin Mingzhen Yang Mingzhen Yang Bowen Wang Bowen Wang Yun Zhang Yun Zhang Ningli Li Ningli Li Qin Li Yingying Li Cory J. Xian Tiejun Li Tiejun Li Yuankun Zhai Yuankun Zhai Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats Frontiers in Pharmacology Eucommia ulmoides total flavonoids osteoporosis Ovx intestinal microbiota uterus |
| title | Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats |
| title_full | Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats |
| title_fullStr | Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats |
| title_full_unstemmed | Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats |
| title_short | Total flavonoids isolated from Eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats |
| title_sort | total flavonoids isolated from eucommia ulmoides can alleviate bone loss and regulate intestinal microbiota in ovariectomized rats |
| topic | Eucommia ulmoides total flavonoids osteoporosis Ovx intestinal microbiota uterus |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1513863/full |
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