Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment
Abstract Osteoarthritis (OA) is a joint disease characterized by inflammation, cartilage degeneration, and pain. Recent studies have focused on the potential of active phenolic compounds as a treatment for OA, owing to their anti-inflammatory, antioxidant, and chondroprotective effects. Specifically...
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Nature Portfolio
2025-07-01
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| author | Mónica Paesa Mauricio David Izquierdo Cristina Remírez de Ganuza Pedro Ferreira-Santos María Jesús Rodríguez-Yoldi Gracia Mendoza |
| author_facet | Mónica Paesa Mauricio David Izquierdo Cristina Remírez de Ganuza Pedro Ferreira-Santos María Jesús Rodríguez-Yoldi Gracia Mendoza |
| author_sort | Mónica Paesa |
| collection | DOAJ |
| description | Abstract Osteoarthritis (OA) is a joint disease characterized by inflammation, cartilage degeneration, and pain. Recent studies have focused on the potential of active phenolic compounds as a treatment for OA, owing to their anti-inflammatory, antioxidant, and chondroprotective effects. Specifically, Boletus edulis (BE) extracts, a natural product from mushrooms, have been shown as significantly active compounds against oxidative stress and inflammation. Our aim was to evaluate the anti-inflammatory and chondroprotective potential of BE extracts, primarily composed of phenolic compounds and flavonoids, in OA in vitro models. Our results showed that cell treatment with 250 µg/mL of BE extracts maintained cell viability above 70% without affecting apoptosis or cell cycle. At the molecular level, BE extracts exerted anti-inflammatory effects by reducing nitric oxide (NO) levels, as well as the expression of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines and chemokines (interleukin-6 (IL-6), IL-8, (C-X-C motif) ligand 1 (CXCL-1)) in lipopolysaccharide (LPS)-stimulated cells. Additionally, BE extracts treatment demonstrated a significant decrease in matrix metalloproteinases 3 and 13 (MMP-3, MMP-13) expression, while avoiding the depletion in the expression of chondrogenic markers, aggrecan and collagen II. Furthermore, inflammation was also significantly reduced through the inhibition of p65 nuclear translocation. These findings suggest that BE extracts may provide a promising basis for the development of novel therapeutic strategies in the treatment of OA, reducing inflammation and chondrocytes damage. |
| format | Article |
| id | doaj-art-32e79963b22442e1abc079bfc60f8ed6 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-32e79963b22442e1abc079bfc60f8ed62025-08-20T03:42:49ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-09822-1Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatmentMónica Paesa0Mauricio David Izquierdo1Cristina Remírez de Ganuza2Pedro Ferreira-Santos3María Jesús Rodríguez-Yoldi4Gracia Mendoza5Department of Chemical Engineering, University of ZaragozaDepartment of Pharmacology and Physiology, Forensic and Legal Medicine, Veterinary Faculty, University of ZaragozaDepartment of Chemical Engineering, University of ZaragozaDepartment of Chemical Engineering, Faculty of Science, University of VigoAragon Health Research Institute (IIS Aragon)Aragon Health Research Institute (IIS Aragon)Abstract Osteoarthritis (OA) is a joint disease characterized by inflammation, cartilage degeneration, and pain. Recent studies have focused on the potential of active phenolic compounds as a treatment for OA, owing to their anti-inflammatory, antioxidant, and chondroprotective effects. Specifically, Boletus edulis (BE) extracts, a natural product from mushrooms, have been shown as significantly active compounds against oxidative stress and inflammation. Our aim was to evaluate the anti-inflammatory and chondroprotective potential of BE extracts, primarily composed of phenolic compounds and flavonoids, in OA in vitro models. Our results showed that cell treatment with 250 µg/mL of BE extracts maintained cell viability above 70% without affecting apoptosis or cell cycle. At the molecular level, BE extracts exerted anti-inflammatory effects by reducing nitric oxide (NO) levels, as well as the expression of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines and chemokines (interleukin-6 (IL-6), IL-8, (C-X-C motif) ligand 1 (CXCL-1)) in lipopolysaccharide (LPS)-stimulated cells. Additionally, BE extracts treatment demonstrated a significant decrease in matrix metalloproteinases 3 and 13 (MMP-3, MMP-13) expression, while avoiding the depletion in the expression of chondrogenic markers, aggrecan and collagen II. Furthermore, inflammation was also significantly reduced through the inhibition of p65 nuclear translocation. These findings suggest that BE extracts may provide a promising basis for the development of novel therapeutic strategies in the treatment of OA, reducing inflammation and chondrocytes damage.https://doi.org/10.1038/s41598-025-09822-1OsteoarthritisBoletus edulis extractPhenolic compoundsATDC-5 cellsChondroprotectiveAnti-inflammatory |
| spellingShingle | Mónica Paesa Mauricio David Izquierdo Cristina Remírez de Ganuza Pedro Ferreira-Santos María Jesús Rodríguez-Yoldi Gracia Mendoza Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment Scientific Reports Osteoarthritis Boletus edulis extract Phenolic compounds ATDC-5 cells Chondroprotective Anti-inflammatory |
| title | Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment |
| title_full | Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment |
| title_fullStr | Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment |
| title_full_unstemmed | Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment |
| title_short | Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment |
| title_sort | unveiling the therapeutic potential of phenolic compounds from boletus edulis in osteoarthritis treatment |
| topic | Osteoarthritis Boletus edulis extract Phenolic compounds ATDC-5 cells Chondroprotective Anti-inflammatory |
| url | https://doi.org/10.1038/s41598-025-09822-1 |
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