False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor
Crossmatching is essential prior to kidney transplantation to confirm compatibility between the donor and the recipient, particularly to prevent acute antibody-mediated rejection. An unexpected positive crossmatch may be obtained in recipients with an autoimmune disease or preexisting antibodies not...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Case Reports in Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2016/4534898 |
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| _version_ | 1832551450695172096 |
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| author | Judith Desoutter Marie-Joëlle Apithy Ségolène Bartczak Nicolas Guillaume |
| author_facet | Judith Desoutter Marie-Joëlle Apithy Ségolène Bartczak Nicolas Guillaume |
| author_sort | Judith Desoutter |
| collection | DOAJ |
| description | Crossmatching is essential prior to kidney transplantation to confirm compatibility between the donor and the recipient, particularly to prevent acute antibody-mediated rejection. An unexpected positive crossmatch may be obtained in recipients with an autoimmune disease or preexisting antibodies not detected by single-antigen bead array due to complement interference or who have been previously treated by desensitization protocols such as rituximab, antithymocyte globulin, or intravenous immunoglobulins. We report donor and recipient investigations that revealed unexpected positive B-cells crossmatch, probably due to donor cells, as the donor had received rituximab therapy shortly before organ harvesting, in a context of severe idiopathic thrombocytopenic purpura. We consequently detected unexpected Class II IgG complement-dependent cytotoxicity for all sera tested. Other laboratory investigations failed to elucidate the reasons for this recipient-related positivity. |
| format | Article |
| id | doaj-art-32e407a53b834b3e951a3771860fb2f4 |
| institution | Kabale University |
| issn | 2090-6943 2090-6951 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Case Reports in Transplantation |
| spelling | doaj-art-32e407a53b834b3e951a3771860fb2f42025-02-03T06:01:21ZengWileyCase Reports in Transplantation2090-69432090-69512016-01-01201610.1155/2016/45348984534898False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney DonorJudith Desoutter0Marie-Joëlle Apithy1Ségolène Bartczak2Nicolas Guillaume3Department of Histocompatibility, Amiens University Medical Center, 80000 Amiens, FranceDepartment of Histocompatibility, Amiens University Medical Center, 80000 Amiens, FranceDepartment of Histocompatibility, Amiens University Medical Center, 80000 Amiens, FranceDepartment of Histocompatibility, Amiens University Medical Center, 80000 Amiens, FranceCrossmatching is essential prior to kidney transplantation to confirm compatibility between the donor and the recipient, particularly to prevent acute antibody-mediated rejection. An unexpected positive crossmatch may be obtained in recipients with an autoimmune disease or preexisting antibodies not detected by single-antigen bead array due to complement interference or who have been previously treated by desensitization protocols such as rituximab, antithymocyte globulin, or intravenous immunoglobulins. We report donor and recipient investigations that revealed unexpected positive B-cells crossmatch, probably due to donor cells, as the donor had received rituximab therapy shortly before organ harvesting, in a context of severe idiopathic thrombocytopenic purpura. We consequently detected unexpected Class II IgG complement-dependent cytotoxicity for all sera tested. Other laboratory investigations failed to elucidate the reasons for this recipient-related positivity.http://dx.doi.org/10.1155/2016/4534898 |
| spellingShingle | Judith Desoutter Marie-Joëlle Apithy Ségolène Bartczak Nicolas Guillaume False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor Case Reports in Transplantation |
| title | False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor |
| title_full | False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor |
| title_fullStr | False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor |
| title_full_unstemmed | False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor |
| title_short | False Positive B-Cells Crossmatch after Prior Rituximab Exposure of the Kidney Donor |
| title_sort | false positive b cells crossmatch after prior rituximab exposure of the kidney donor |
| url | http://dx.doi.org/10.1155/2016/4534898 |
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