Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity
Background: This study investigated the ability of <i>Enterococcus lactis</i> (<i>E. lactis</i>) and Hasawi rice protein lysate (HPL) to suppress colitis induced by dextran sulfate sodium (DSS) in miceColitis is characterized by inflammation of the colon, and exploring potent...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/17/11/1463 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850267036795535360 |
|---|---|
| author | Ashraf Khalifa Mayyadah Abdullah Alkuwayti Basem M. Abdallah Enas M. Ali Hairul Islam M. Ibrahim |
| author_facet | Ashraf Khalifa Mayyadah Abdullah Alkuwayti Basem M. Abdallah Enas M. Ali Hairul Islam M. Ibrahim |
| author_sort | Ashraf Khalifa |
| collection | DOAJ |
| description | Background: This study investigated the ability of <i>Enterococcus lactis</i> (<i>E. lactis</i>) and Hasawi rice protein lysate (HPL) to suppress colitis induced by dextran sulfate sodium (DSS) in miceColitis is characterized by inflammation of the colon, and exploring potential therapeutic agents could lead to improved management strategies. Methods: Male mice were subjected to DSS treatment to induce colitis, followed by supplementation with <i>E. lactis</i> and/or HPL. The study assessed various parameters, including disease activity index (DAI) scores, gut permeability measured using FITC-dextran, and superoxide dismutase (SOD) activity in excised colon tissues from both treated and untreated control groups. Results: <i>E. lactis</i> supplementation significantly alleviated DSS-induced colitis, as evidenced by improved DAI scores and enhanced gut permeability. Notably, <i>E. lactis</i> combined with HPL (0.1 mg/10<sup>8</sup>) exhibited superior tolerance to a 0.5% pancreatin solution compared to <i>E. lactis</i> alone. Both <i>E. lactis</i> and the combination treatment significantly increased SOD activity (5.6 ± 0.23 SOD U/mg protein for <i>E. lactis</i> and 6.7 ± 0.23 SOD U/mg protein for the combination) relative to the Azoxymethane (AOM)/DSS group, suggesting a reduction in oxidative stress. Additionally, pro-inflammatory markers were significantly reduced in the group receiving both <i>E. lactis</i> and HPL compared to the E. lactis-only group. Levels of proteins associated with cell death, such as PCNA, PTEN, VEGF, COX-2, and STAT-3, were significantly decreased by 14.8% to 80% following <i>E. lactis</i> supplementation, with the combination treatment showing the most pronounced effects. Conclusions: These findings suggest <i>E. lactis</i> supplementation may be beneficial for colitis, with HPL potential to enhance its effectiveness. |
| format | Article |
| id | doaj-art-32e25cf4fd1f407fa44885cf078f9987 |
| institution | OA Journals |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-32e25cf4fd1f407fa44885cf078f99872025-08-20T01:53:57ZengMDPI AGPharmaceuticals1424-82472024-10-011711146310.3390/ph17111463Probiotic and Rice-Derived Compound Combination Mitigates Colitis SeverityAshraf Khalifa0Mayyadah Abdullah Alkuwayti1Basem M. Abdallah2Enas M. Ali3Hairul Islam M. Ibrahim4Biological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaBiological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaBiological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaBiological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaBiological Science Department, College of Science, King Faisal University, P.O. Box 400, Al-Ahsa 31982, Saudi ArabiaBackground: This study investigated the ability of <i>Enterococcus lactis</i> (<i>E. lactis</i>) and Hasawi rice protein lysate (HPL) to suppress colitis induced by dextran sulfate sodium (DSS) in miceColitis is characterized by inflammation of the colon, and exploring potential therapeutic agents could lead to improved management strategies. Methods: Male mice were subjected to DSS treatment to induce colitis, followed by supplementation with <i>E. lactis</i> and/or HPL. The study assessed various parameters, including disease activity index (DAI) scores, gut permeability measured using FITC-dextran, and superoxide dismutase (SOD) activity in excised colon tissues from both treated and untreated control groups. Results: <i>E. lactis</i> supplementation significantly alleviated DSS-induced colitis, as evidenced by improved DAI scores and enhanced gut permeability. Notably, <i>E. lactis</i> combined with HPL (0.1 mg/10<sup>8</sup>) exhibited superior tolerance to a 0.5% pancreatin solution compared to <i>E. lactis</i> alone. Both <i>E. lactis</i> and the combination treatment significantly increased SOD activity (5.6 ± 0.23 SOD U/mg protein for <i>E. lactis</i> and 6.7 ± 0.23 SOD U/mg protein for the combination) relative to the Azoxymethane (AOM)/DSS group, suggesting a reduction in oxidative stress. Additionally, pro-inflammatory markers were significantly reduced in the group receiving both <i>E. lactis</i> and HPL compared to the E. lactis-only group. Levels of proteins associated with cell death, such as PCNA, PTEN, VEGF, COX-2, and STAT-3, were significantly decreased by 14.8% to 80% following <i>E. lactis</i> supplementation, with the combination treatment showing the most pronounced effects. Conclusions: These findings suggest <i>E. lactis</i> supplementation may be beneficial for colitis, with HPL potential to enhance its effectiveness.https://www.mdpi.com/1424-8247/17/11/1463<i>Enterococcus lactis</i>Al-Asfar Lakeinflammatory disordersHasawi riceprotein hydrolysate |
| spellingShingle | Ashraf Khalifa Mayyadah Abdullah Alkuwayti Basem M. Abdallah Enas M. Ali Hairul Islam M. Ibrahim Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity Pharmaceuticals <i>Enterococcus lactis</i> Al-Asfar Lake inflammatory disorders Hasawi rice protein hydrolysate |
| title | Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity |
| title_full | Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity |
| title_fullStr | Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity |
| title_full_unstemmed | Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity |
| title_short | Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity |
| title_sort | probiotic and rice derived compound combination mitigates colitis severity |
| topic | <i>Enterococcus lactis</i> Al-Asfar Lake inflammatory disorders Hasawi rice protein hydrolysate |
| url | https://www.mdpi.com/1424-8247/17/11/1463 |
| work_keys_str_mv | AT ashrafkhalifa probioticandricederivedcompoundcombinationmitigatescolitisseverity AT mayyadahabdullahalkuwayti probioticandricederivedcompoundcombinationmitigatescolitisseverity AT basemmabdallah probioticandricederivedcompoundcombinationmitigatescolitisseverity AT enasmali probioticandricederivedcompoundcombinationmitigatescolitisseverity AT hairulislammibrahim probioticandricederivedcompoundcombinationmitigatescolitisseverity |