Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry
Flow cytometry is a conceptually straightforward quantitative method that can be used to determine the DNA content (and many other parameters) of individual cells. It has been used in chronic ulcerative colitis to evaluate the presence of aneuploidy as a marker of colon cancer risk. In this paper, t...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1990-01-01
|
| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/1990/981048 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849387140776984576 |
|---|---|
| author | DJ Ahnen JB McHugh JM Bozdech GH Warren |
| author_facet | DJ Ahnen JB McHugh JM Bozdech GH Warren |
| author_sort | DJ Ahnen |
| collection | DOAJ |
| description | Flow cytometry is a conceptually straightforward quantitative
method that can be used to determine the DNA content (and many other
parameters) of individual cells. It has been used in chronic ulcerative colitis to
evaluate the presence of aneuploidy as a marker of colon cancer risk. In this paper,
the basic principles of flow cytometry are reviewed, the current status of published
studies using flow cytometric analysis of DNA content in chronic ulcerative
colitis outlined, conclusions from the research are proposed and future research
needs identified. Overall, aneuploidy correlates with the current best markers of
cancer risk in ulcerative colitis, ie, dysplasia and established cancer. Whether
aneuplo1dy will be useful for the prospective identification of individuals at
increased risk for colon cancer deserves rigorous evaluation. An important
technical issue of potential variability in interpretation of near diploid peaks is
emphasized. |
| format | Article |
| id | doaj-art-32ce6b4b4d0042d2bde8567fa777579d |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 1990-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-32ce6b4b4d0042d2bde8567fa777579d2025-08-20T03:55:22ZengWileyCanadian Journal of Gastroenterology0835-79001990-01-014739039610.1155/1990/981048Carcinogenesis in Chronic Ulcerative Colitis: Flow CytometryDJ Ahnen0JB McHugh1JM Bozdech2GH Warren3Department of Medicine, Denver Veterans Administration Medical Cencer, University of Colorado Health Sciences Cencer, USADepartment of Medicine, Denver Veterans Administration Medical Cencer, University of Colorado Health Sciences Cencer, USADepartment of Medicine, Cleveland Clinic Foundation, Cleveland, Ohio, USADepartment of Medicine, Denver Veterans Administration Medical Cencer, University of Colorado Health Sciences Cencer, USAFlow cytometry is a conceptually straightforward quantitative method that can be used to determine the DNA content (and many other parameters) of individual cells. It has been used in chronic ulcerative colitis to evaluate the presence of aneuploidy as a marker of colon cancer risk. In this paper, the basic principles of flow cytometry are reviewed, the current status of published studies using flow cytometric analysis of DNA content in chronic ulcerative colitis outlined, conclusions from the research are proposed and future research needs identified. Overall, aneuploidy correlates with the current best markers of cancer risk in ulcerative colitis, ie, dysplasia and established cancer. Whether aneuplo1dy will be useful for the prospective identification of individuals at increased risk for colon cancer deserves rigorous evaluation. An important technical issue of potential variability in interpretation of near diploid peaks is emphasized.http://dx.doi.org/10.1155/1990/981048 |
| spellingShingle | DJ Ahnen JB McHugh JM Bozdech GH Warren Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry Canadian Journal of Gastroenterology |
| title | Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry |
| title_full | Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry |
| title_fullStr | Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry |
| title_full_unstemmed | Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry |
| title_short | Carcinogenesis in Chronic Ulcerative Colitis: Flow Cytometry |
| title_sort | carcinogenesis in chronic ulcerative colitis flow cytometry |
| url | http://dx.doi.org/10.1155/1990/981048 |
| work_keys_str_mv | AT djahnen carcinogenesisinchroniculcerativecolitisflowcytometry AT jbmchugh carcinogenesisinchroniculcerativecolitisflowcytometry AT jmbozdech carcinogenesisinchroniculcerativecolitisflowcytometry AT ghwarren carcinogenesisinchroniculcerativecolitisflowcytometry |