Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma
ABSTRACT Purpose: To evaluate peripheral blood mononuclear cells (PBMCs) from patients with advanced head and neck squamous cell carcinoma (HNSCC) in comparison with healthy volunteers, as they can be potential biomarkers. Methods: Immunophenotyping was performed using flow cytometry of blood mono...
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2025-08-01
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| Series: | Acta Cirúrgica Brasileira |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100806&tlng=en |
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| author | Adriana Torres da Silva Érica Leandro Marciano Vieira Ana Cristina Simões e Silva Andy Petroianu |
| author_facet | Adriana Torres da Silva Érica Leandro Marciano Vieira Ana Cristina Simões e Silva Andy Petroianu |
| author_sort | Adriana Torres da Silva |
| collection | DOAJ |
| description | ABSTRACT Purpose: To evaluate peripheral blood mononuclear cells (PBMCs) from patients with advanced head and neck squamous cell carcinoma (HNSCC) in comparison with healthy volunteers, as they can be potential biomarkers. Methods: Immunophenotyping was performed using flow cytometry of blood mononuclear cells from two groups of adult men: group 1 (n = 14), diagnosed with HNSCC (mouth, larynx, and hypopharynx); and group 2 (n = 14), volunteers, healthy, and without the use of drugs. The cell groups studied were T lymphocytes (CD3, CD4, CD8, CD56 and CD69), B lymphocytes (CD19, CD69), neutrophils (CD11a, CD16, CD66b, HLA-DR), and monocytes (CD14, CD86). Results: In group 1, there were an increase in CD3+CD4+ T lymphocytes (p < 0.001) and NK 56+ cells (p = 0.009) and a decrease in CD3+CD8+ T lymphocytes (p = 0.02) in comparison with group 2. In patients with HNSCC, an increase was found in the expression of the CD69 marker in CD3+CD4+ T lymphocytes (p = 0.03) and CD19+ B lymphocytes (p = 0.01) when compared to healthy volunteers. Conclusion: HNSCC triggers a systemic inflammatory response with a decrease in CD8 T cells and an increase in CD4 T and CD56 natural killer cells. CD69 early activation marker was expressed by T and B cells. |
| format | Article |
| id | doaj-art-32c832c73a214e2baad5316843e05e6e |
| institution | DOAJ |
| issn | 1678-2674 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj-art-32c832c73a214e2baad5316843e05e6e2025-08-20T03:03:50ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742025-08-014010.1590/acb405425Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinomaAdriana Torres da Silvahttps://orcid.org/0000-0002-7258-7176Érica Leandro Marciano Vieirahttps://orcid.org/0000-0002-4147-5614Ana Cristina Simões e Silvahttps://orcid.org/0000-0001-9222-3882Andy Petroianuhttps://orcid.org/0000-0002-8659-6866ABSTRACT Purpose: To evaluate peripheral blood mononuclear cells (PBMCs) from patients with advanced head and neck squamous cell carcinoma (HNSCC) in comparison with healthy volunteers, as they can be potential biomarkers. Methods: Immunophenotyping was performed using flow cytometry of blood mononuclear cells from two groups of adult men: group 1 (n = 14), diagnosed with HNSCC (mouth, larynx, and hypopharynx); and group 2 (n = 14), volunteers, healthy, and without the use of drugs. The cell groups studied were T lymphocytes (CD3, CD4, CD8, CD56 and CD69), B lymphocytes (CD19, CD69), neutrophils (CD11a, CD16, CD66b, HLA-DR), and monocytes (CD14, CD86). Results: In group 1, there were an increase in CD3+CD4+ T lymphocytes (p < 0.001) and NK 56+ cells (p = 0.009) and a decrease in CD3+CD8+ T lymphocytes (p = 0.02) in comparison with group 2. In patients with HNSCC, an increase was found in the expression of the CD69 marker in CD3+CD4+ T lymphocytes (p = 0.03) and CD19+ B lymphocytes (p = 0.01) when compared to healthy volunteers. Conclusion: HNSCC triggers a systemic inflammatory response with a decrease in CD8 T cells and an increase in CD4 T and CD56 natural killer cells. CD69 early activation marker was expressed by T and B cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100806&tlng=enCarcinoma, Squamous CellHead and Neck NeoplasmsImmunophenotypingLymphocytesLeukocytes, Mononuclear |
| spellingShingle | Adriana Torres da Silva Érica Leandro Marciano Vieira Ana Cristina Simões e Silva Andy Petroianu Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma Acta Cirúrgica Brasileira Carcinoma, Squamous Cell Head and Neck Neoplasms Immunophenotyping Lymphocytes Leukocytes, Mononuclear |
| title | Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma |
| title_full | Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma |
| title_fullStr | Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma |
| title_full_unstemmed | Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma |
| title_short | Expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma |
| title_sort | expression of immunophenotypic markers in blood mononuclear cells of patients with advanced head and neck squamous cells carcinoma |
| topic | Carcinoma, Squamous Cell Head and Neck Neoplasms Immunophenotyping Lymphocytes Leukocytes, Mononuclear |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502025000100806&tlng=en |
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