Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails

The trial investigated the effects of anise seed powder and protease, alone and combined, in Japanese quail diets. 540 seven-day-old quails were divided into four groups: Control (basal diet), Anise Seed Group (1000 mg/kg anise seed powder), Protease Enzyme Group (30,000 IU/kg Bacillus subtilis-deri...

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Main Authors: Asad Sultan, Sidra Bibi, Shabana Naz, Ziaul Islam, Abdulwahed Fahad Alrefaei, Rifat Ullah Khan, Samia H. Abdelrahman, Maria Selvaggi
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Food and Agricultural Immunology
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Online Access:https://www.tandfonline.com/doi/10.1080/09540105.2024.2351362
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author Asad Sultan
Sidra Bibi
Shabana Naz
Ziaul Islam
Abdulwahed Fahad Alrefaei
Rifat Ullah Khan
Samia H. Abdelrahman
Maria Selvaggi
author_facet Asad Sultan
Sidra Bibi
Shabana Naz
Ziaul Islam
Abdulwahed Fahad Alrefaei
Rifat Ullah Khan
Samia H. Abdelrahman
Maria Selvaggi
author_sort Asad Sultan
collection DOAJ
description The trial investigated the effects of anise seed powder and protease, alone and combined, in Japanese quail diets. 540 seven-day-old quails were divided into four groups: Control (basal diet), Anise Seed Group (1000 mg/kg anise seed powder), Protease Enzyme Group (30,000 IU/kg Bacillus subtilis-derived protease enzyme), and a combination of anise powder and protease. The combination significantly increased weight gain and dressing percentage compared to the control. Quails receiving protease, anise powder, or both showed higher antibody titers against infectious diseases. The population of beneficial Lactobacillus increased in groups receiving protease and the combination. Additionally, the presence of harmful bacteria like Escherichia coli and Salmonella decreased significantly in quails supplemented with both protease enzyme and anise powder. In summary, combining protease and anise powder improved growth performance, immune responses, increased Lactobacillus population, and decreased pathogenic bacteria in quails.
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issn 0954-0105
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language English
publishDate 2024-12-01
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series Food and Agricultural Immunology
spelling doaj-art-32b57ee3db254b3b89baa381b11f3a932025-08-20T02:52:13ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432024-12-0135110.1080/09540105.2024.2351362Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quailsAsad Sultan0Sidra Bibi1Shabana Naz2Ziaul Islam3Abdulwahed Fahad Alrefaei4Rifat Ullah Khan5Samia H. Abdelrahman6Maria Selvaggi7Department of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, PakistanDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, PakistanDepartment of Zoology, Government College University, Faisalabad, PakistanDepartment of Animal Sciences, Shaheed Benazir Bhutto University Sheringal, Dir Upper, PakistanDepartment of Zoology, College of Sciences, King Saud University, Riyadh, Saudi ArabiaCollege of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, PakistanCentre of Veterinary Research, Khartum, SudanDepartment of Soil, Plant and Food Science, University of Bari “Aldo Moro”, Bari, ItalyThe trial investigated the effects of anise seed powder and protease, alone and combined, in Japanese quail diets. 540 seven-day-old quails were divided into four groups: Control (basal diet), Anise Seed Group (1000 mg/kg anise seed powder), Protease Enzyme Group (30,000 IU/kg Bacillus subtilis-derived protease enzyme), and a combination of anise powder and protease. The combination significantly increased weight gain and dressing percentage compared to the control. Quails receiving protease, anise powder, or both showed higher antibody titers against infectious diseases. The population of beneficial Lactobacillus increased in groups receiving protease and the combination. Additionally, the presence of harmful bacteria like Escherichia coli and Salmonella decreased significantly in quails supplemented with both protease enzyme and anise powder. In summary, combining protease and anise powder improved growth performance, immune responses, increased Lactobacillus population, and decreased pathogenic bacteria in quails.https://www.tandfonline.com/doi/10.1080/09540105.2024.2351362Japanese quailsproteaseanise seed powdergrowthmicrobiotadigestibility
spellingShingle Asad Sultan
Sidra Bibi
Shabana Naz
Ziaul Islam
Abdulwahed Fahad Alrefaei
Rifat Ullah Khan
Samia H. Abdelrahman
Maria Selvaggi
Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails
Food and Agricultural Immunology
Japanese quails
protease
anise seed powder
growth
microbiota
digestibility
title Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails
title_full Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails
title_fullStr Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails
title_full_unstemmed Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails
title_short Effect of anise seed (Pimpinella anisum L.) and protease alone or in combination on growth performance, carcase characteristics, humoral immunity, nutrients digestibility and cecal microbiota in Japanese quails
title_sort effect of anise seed pimpinella anisum l and protease alone or in combination on growth performance carcase characteristics humoral immunity nutrients digestibility and cecal microbiota in japanese quails
topic Japanese quails
protease
anise seed powder
growth
microbiota
digestibility
url https://www.tandfonline.com/doi/10.1080/09540105.2024.2351362
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