Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism
Electroacupuncture (EA) has shown promise as a treatment for Functional constipation (FC), with growing evidence suggesting it may enhance gut motility. MicroRNAs (miRNAs) serve as key regulatory molecules mediating host-microbiota interactions. However, the specific fecal miRNAs regulating microbio...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1517018/full |
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| author | Lu Wang Lu Wang Menghan Xi Menghan Xi Wei Cao Haiyan Qin Di Qin Shuai Chen Siyuan Zhou Yujun Hou Ying Chen Xianjun Xiao Qianhua Zheng Dehua Li Ying Li |
| author_facet | Lu Wang Lu Wang Menghan Xi Menghan Xi Wei Cao Haiyan Qin Di Qin Shuai Chen Siyuan Zhou Yujun Hou Ying Chen Xianjun Xiao Qianhua Zheng Dehua Li Ying Li |
| author_sort | Lu Wang |
| collection | DOAJ |
| description | Electroacupuncture (EA) has shown promise as a treatment for Functional constipation (FC), with growing evidence suggesting it may enhance gut motility. MicroRNAs (miRNAs) serve as key regulatory molecules mediating host-microbiota interactions. However, the specific fecal miRNAs regulating microbiota composition and metabolism in EA-treated constipated mice, along with their key targets, remain unidentified. We examined fecal microbiome composition, metabolism, and colonic miRNA expression in loperamide-induced constipated mice and EA-treated mice to identify differentially expressed miRNAs and assess their relationships with microbial abundance, metabolism, and gut motility. An antibiotic cocktail and adeno-associated virus were employed to interfere with the gut microbiota and target miRNA in vivo, thereby validating the proposed mechanism. Our results indicate that miR-205-5p, significantly upregulated in fecal and colonic tissues of EA-treated constipated mice, promotes intestinal motility in a microbiome-dependent manner. Specifically, EA promoted the growth of Lactobacillus reuteri, enriched in the feces of constipation-recovered mice, through host-derived miR-205-5p regulation. Furthermore, Lactobacillus reuteri and its tryptophan metabolites (indole-3-acetamide, indole-3-acetic acid, and indole-3-carboxaldehyde) alleviated loperamide-induced constipation. These findings underscore the pivotal role of host-derived miR-205-5p in modulating microbial composition and tryptophan metabolites to enhance intestinal motility through EA. |
| format | Article |
| id | doaj-art-32b30d4ce00643c2b2ac67a91cb78555 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-32b30d4ce00643c2b2ac67a91cb785552025-02-05T07:32:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15170181517018Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolismLu Wang0Lu Wang1Menghan Xi2Menghan Xi3Wei Cao4Haiyan Qin5Di Qin6Shuai Chen7Siyuan Zhou8Yujun Hou9Ying Chen10Xianjun Xiao11Qianhua Zheng12Dehua Li13Ying Li14Department of Acupuncture, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaDepartment of Acupuncture and Moxibustion, Chengdu Pidu District Hospital of TCM/The Third Clinical Medical College of Chengdu University of TCM, Chengdu, Sichuan, ChinaCenter of Preventive Medicine, Chengdu Integrated TCM and Western Medicine Hospital, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaSchool of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaDepartment of Acupuncture, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaAcupuncture and Tuina School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, ChinaElectroacupuncture (EA) has shown promise as a treatment for Functional constipation (FC), with growing evidence suggesting it may enhance gut motility. MicroRNAs (miRNAs) serve as key regulatory molecules mediating host-microbiota interactions. However, the specific fecal miRNAs regulating microbiota composition and metabolism in EA-treated constipated mice, along with their key targets, remain unidentified. We examined fecal microbiome composition, metabolism, and colonic miRNA expression in loperamide-induced constipated mice and EA-treated mice to identify differentially expressed miRNAs and assess their relationships with microbial abundance, metabolism, and gut motility. An antibiotic cocktail and adeno-associated virus were employed to interfere with the gut microbiota and target miRNA in vivo, thereby validating the proposed mechanism. Our results indicate that miR-205-5p, significantly upregulated in fecal and colonic tissues of EA-treated constipated mice, promotes intestinal motility in a microbiome-dependent manner. Specifically, EA promoted the growth of Lactobacillus reuteri, enriched in the feces of constipation-recovered mice, through host-derived miR-205-5p regulation. Furthermore, Lactobacillus reuteri and its tryptophan metabolites (indole-3-acetamide, indole-3-acetic acid, and indole-3-carboxaldehyde) alleviated loperamide-induced constipation. These findings underscore the pivotal role of host-derived miR-205-5p in modulating microbial composition and tryptophan metabolites to enhance intestinal motility through EA.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1517018/fullelectroacupuncturefunctional constipationmiR-205-5pLactobacillus reuteritryptophan metabolism |
| spellingShingle | Lu Wang Lu Wang Menghan Xi Menghan Xi Wei Cao Haiyan Qin Di Qin Shuai Chen Siyuan Zhou Yujun Hou Ying Chen Xianjun Xiao Qianhua Zheng Dehua Li Ying Li Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism Frontiers in Microbiology electroacupuncture functional constipation miR-205-5p Lactobacillus reuteri tryptophan metabolism |
| title | Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism |
| title_full | Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism |
| title_fullStr | Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism |
| title_full_unstemmed | Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism |
| title_short | Electroacupuncture alleviates functional constipation by upregulating host-derived miR-205-5p to modulate gut microbiota and tryptophan metabolism |
| title_sort | electroacupuncture alleviates functional constipation by upregulating host derived mir 205 5p to modulate gut microbiota and tryptophan metabolism |
| topic | electroacupuncture functional constipation miR-205-5p Lactobacillus reuteri tryptophan metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1517018/full |
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