COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.

<h4>Background</h4>Malaria is a common and severe public health problem in Ghana and largely responsible for febrile symptoms presented at health facilities in the country. Other infectious diseases, including COVID-19, may mimic malaria due to their shared non-specific symptoms such as...

Full description

Saved in:
Bibliographic Details
Main Authors: Issabella Asamoah, Mildred Adusei-Poku, Priscilla Vandyck-Sey, Allen Steele-Dadzie, Atta Senior Kuffour, Albert Turkson, Ivy Asantewaa Asante, Kantanka Addo-Osafo, Quaneeta Mohktar, Bright Adu, Yaw A Afrane, Kwamena W C Sagoe
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0298088&type=printable
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850081738552770560
author Issabella Asamoah
Mildred Adusei-Poku
Priscilla Vandyck-Sey
Allen Steele-Dadzie
Atta Senior Kuffour
Albert Turkson
Ivy Asantewaa Asante
Kantanka Addo-Osafo
Quaneeta Mohktar
Bright Adu
Yaw A Afrane
Kwamena W C Sagoe
author_facet Issabella Asamoah
Mildred Adusei-Poku
Priscilla Vandyck-Sey
Allen Steele-Dadzie
Atta Senior Kuffour
Albert Turkson
Ivy Asantewaa Asante
Kantanka Addo-Osafo
Quaneeta Mohktar
Bright Adu
Yaw A Afrane
Kwamena W C Sagoe
author_sort Issabella Asamoah
collection DOAJ
description <h4>Background</h4>Malaria is a common and severe public health problem in Ghana and largely responsible for febrile symptoms presented at health facilities in the country. Other infectious diseases, including COVID-19, may mimic malaria due to their shared non-specific symptoms such as fever and headache thus leading to misdiagnosis. This study therefore investigated COVID-19 among patients presenting with malaria-like symptoms at Korle-Bu Polyclinic, Accra, Ghana.<h4>Methods</h4>This study enrolled 300 patients presenting with malaria-like symptoms aged ≥18yrs. After consent was obtained from study patients, two to three millilitres of whole blood, nasopharyngeal and oropharyngeal swab samples, were collected for screening of Plasmodium falciparum using malaria rapid diagnostic test, microscopy and nested PCR, and SARS-CoV-2 using SARS-CoV-2 antigen test and Real-time PCR, respectively. The plasma and whole blood were also used for COVID-19 antibody testing and full blood counts using hematological analyser. SARS-CoV-2 whole genome sequencing was performed using MinIon sequencing.<h4>Results</h4>The prevalence of malaria by microscopy, RDT and nested PCR were 2.3%, 2.3% and 2.7% respectively. The detection of SARS-CoV-2 by COVID-19 Rapid Antigen Test and Real-time PCR were 8.7% and 20% respectively. The Delta variant was reported in 23 of 25 SARS-CoV-2 positives with CT values below 30. Headache was the most common symptom presented by study participants (95%). Comorbidities reported were hypertension, asthma and diabetes. One hundred and thirteen (37.8%) of the study participants had prior exposure to SARS CoV-2 and (34/51) 66.7% of Astrazeneca vaccinated patients had no IgG antibody.<h4>Conclusion</h4>It may be difficult to use clinical characteristics to distinguish between patients with COVID-19 having malaria-like symptoms. Detection of IgM using RDTs may be useful in predicting CT values for SARS-CoV-2 real-time PCR and therefore transmission.
format Article
id doaj-art-32aeb98cbb1449e38afb142515ce22e2
institution DOAJ
issn 1932-6203
language English
publishDate 2024-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-32aeb98cbb1449e38afb142515ce22e22025-08-20T02:44:40ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01192e029808810.1371/journal.pone.0298088COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.Issabella AsamoahMildred Adusei-PokuPriscilla Vandyck-SeyAllen Steele-DadzieAtta Senior KuffourAlbert TurksonIvy Asantewaa AsanteKantanka Addo-OsafoQuaneeta MohktarBright AduYaw A AfraneKwamena W C Sagoe<h4>Background</h4>Malaria is a common and severe public health problem in Ghana and largely responsible for febrile symptoms presented at health facilities in the country. Other infectious diseases, including COVID-19, may mimic malaria due to their shared non-specific symptoms such as fever and headache thus leading to misdiagnosis. This study therefore investigated COVID-19 among patients presenting with malaria-like symptoms at Korle-Bu Polyclinic, Accra, Ghana.<h4>Methods</h4>This study enrolled 300 patients presenting with malaria-like symptoms aged ≥18yrs. After consent was obtained from study patients, two to three millilitres of whole blood, nasopharyngeal and oropharyngeal swab samples, were collected for screening of Plasmodium falciparum using malaria rapid diagnostic test, microscopy and nested PCR, and SARS-CoV-2 using SARS-CoV-2 antigen test and Real-time PCR, respectively. The plasma and whole blood were also used for COVID-19 antibody testing and full blood counts using hematological analyser. SARS-CoV-2 whole genome sequencing was performed using MinIon sequencing.<h4>Results</h4>The prevalence of malaria by microscopy, RDT and nested PCR were 2.3%, 2.3% and 2.7% respectively. The detection of SARS-CoV-2 by COVID-19 Rapid Antigen Test and Real-time PCR were 8.7% and 20% respectively. The Delta variant was reported in 23 of 25 SARS-CoV-2 positives with CT values below 30. Headache was the most common symptom presented by study participants (95%). Comorbidities reported were hypertension, asthma and diabetes. One hundred and thirteen (37.8%) of the study participants had prior exposure to SARS CoV-2 and (34/51) 66.7% of Astrazeneca vaccinated patients had no IgG antibody.<h4>Conclusion</h4>It may be difficult to use clinical characteristics to distinguish between patients with COVID-19 having malaria-like symptoms. Detection of IgM using RDTs may be useful in predicting CT values for SARS-CoV-2 real-time PCR and therefore transmission.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0298088&type=printable
spellingShingle Issabella Asamoah
Mildred Adusei-Poku
Priscilla Vandyck-Sey
Allen Steele-Dadzie
Atta Senior Kuffour
Albert Turkson
Ivy Asantewaa Asante
Kantanka Addo-Osafo
Quaneeta Mohktar
Bright Adu
Yaw A Afrane
Kwamena W C Sagoe
COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
PLoS ONE
title COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
title_full COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
title_fullStr COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
title_full_unstemmed COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
title_short COVID-19 in patients presenting with malaria-like symptoms at a primary healthcare facility in Accra, Ghana.
title_sort covid 19 in patients presenting with malaria like symptoms at a primary healthcare facility in accra ghana
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0298088&type=printable
work_keys_str_mv AT issabellaasamoah covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT mildredaduseipoku covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT priscillavandycksey covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT allensteeledadzie covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT attaseniorkuffour covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT albertturkson covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT ivyasantewaaasante covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT kantankaaddoosafo covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT quaneetamohktar covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT brightadu covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT yawaafrane covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana
AT kwamenawcsagoe covid19inpatientspresentingwithmalarialikesymptomsataprimaryhealthcarefacilityinaccraghana