The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity

The Yangtze River Delta (YRD) is one of the most important <i>japonica</i> rice planting areas in China. Balancing the resistance, yield, and quality has always been a core issue in rice breeding due to the negative correlation among these three factors, while the broad-spectrum blast re...

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Main Authors: Shengyuan Zeng, Cancan Du, Yihao Yang, Qingfeng Hu, Chuang Li, Fang Feng, Min Guo, Dedao Jing, Tianzi Lin, Hongbing Gong, Changjie Yan
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/12/1836
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author Shengyuan Zeng
Cancan Du
Yihao Yang
Qingfeng Hu
Chuang Li
Fang Feng
Min Guo
Dedao Jing
Tianzi Lin
Hongbing Gong
Changjie Yan
author_facet Shengyuan Zeng
Cancan Du
Yihao Yang
Qingfeng Hu
Chuang Li
Fang Feng
Min Guo
Dedao Jing
Tianzi Lin
Hongbing Gong
Changjie Yan
author_sort Shengyuan Zeng
collection DOAJ
description The Yangtze River Delta (YRD) is one of the most important <i>japonica</i> rice planting areas in China. Balancing the resistance, yield, and quality has always been a core issue in rice breeding due to the negative correlation among these three factors, while the broad-spectrum blast resistance gene <i>Piz</i> is closely linked with <i>Hd1</i>, the major gene regulating days to heading (DTH), and a precise combination of their beneficial alleles plays a key role in synchronously improving blast resistance and the regional adaptability of <i>japonica</i> rice in YRD. In this study, using the backcross progeny population derived from backbone parent ZD9471 and W1063, two alleles of <i>Hd1</i> were identified. Then, through molecular marker-assisted selection combined with Green Super Rice 40K (GSR40K) chip-based screening, six introgression lines (ILs) with two different alleles combinations of <i>Hd1</i> and <i>Pigm</i> were obtained. An evaluation of the blast resistance, yield, and quality traits showed that compared with the recipient parent, the panicle blast resistance of ILs was significantly enhanced; the grain number per panicle increased consistently with the delaying of the growth period, leading to higher yield in the ILs; the grain quality were synchronously improved. Two representative lines with similar genetic backgrounds but a significantly different regional adaptability, exhibiting a good blast resistance, high yield, and prominent quality were approved and demonstrated promising application prospects.
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institution Kabale University
issn 2223-7747
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publishDate 2025-06-01
publisher MDPI AG
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series Plants
spelling doaj-art-32aae6868b9f4c2badfab7691b8dc0252025-08-20T03:29:51ZengMDPI AGPlants2223-77472025-06-011412183610.3390/plants14121836The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome CollinearityShengyuan Zeng0Cancan Du1Yihao Yang2Qingfeng Hu3Chuang Li4Fang Feng5Min Guo6Dedao Jing7Tianzi Lin8Hongbing Gong9Changjie Yan10Agricultural College, Yangzhou University, Yangzhou 225009, ChinaZhenjiang Academic of Agricultural Sciences, Zhenjiang 212400, ChinaAgricultural College, Yangzhou University, Yangzhou 225009, ChinaZhenjiang Academic of Agricultural Sciences, Zhenjiang 212400, ChinaZhenjiang Academic of Agricultural Sciences, Zhenjiang 212400, ChinaWuhan Greenfafa Institute of Novel Genechip R&D Co., Ltd., Wuhan 430070, ChinaAgricultural College, Yangzhou University, Yangzhou 225009, ChinaZhenjiang Academic of Agricultural Sciences, Zhenjiang 212400, ChinaZhenjiang Academic of Agricultural Sciences, Zhenjiang 212400, ChinaZhenjiang Academic of Agricultural Sciences, Zhenjiang 212400, ChinaAgricultural College, Yangzhou University, Yangzhou 225009, ChinaThe Yangtze River Delta (YRD) is one of the most important <i>japonica</i> rice planting areas in China. Balancing the resistance, yield, and quality has always been a core issue in rice breeding due to the negative correlation among these three factors, while the broad-spectrum blast resistance gene <i>Piz</i> is closely linked with <i>Hd1</i>, the major gene regulating days to heading (DTH), and a precise combination of their beneficial alleles plays a key role in synchronously improving blast resistance and the regional adaptability of <i>japonica</i> rice in YRD. In this study, using the backcross progeny population derived from backbone parent ZD9471 and W1063, two alleles of <i>Hd1</i> were identified. Then, through molecular marker-assisted selection combined with Green Super Rice 40K (GSR40K) chip-based screening, six introgression lines (ILs) with two different alleles combinations of <i>Hd1</i> and <i>Pigm</i> were obtained. An evaluation of the blast resistance, yield, and quality traits showed that compared with the recipient parent, the panicle blast resistance of ILs was significantly enhanced; the grain number per panicle increased consistently with the delaying of the growth period, leading to higher yield in the ILs; the grain quality were synchronously improved. Two representative lines with similar genetic backgrounds but a significantly different regional adaptability, exhibiting a good blast resistance, high yield, and prominent quality were approved and demonstrated promising application prospects.https://www.mdpi.com/2223-7747/14/12/1836<i>Japonica</i> riceblast resistanceyieldquality<i>Pigm</i><i>Hd1</i>
spellingShingle Shengyuan Zeng
Cancan Du
Yihao Yang
Qingfeng Hu
Chuang Li
Fang Feng
Min Guo
Dedao Jing
Tianzi Lin
Hongbing Gong
Changjie Yan
The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity
Plants
<i>Japonica</i> rice
blast resistance
yield
quality
<i>Pigm</i>
<i>Hd1</i>
title The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity
title_full The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity
title_fullStr The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity
title_full_unstemmed The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity
title_short The Molecular Breeding of Different Ecotype <i>Japonica</i> Varieties Resistant to Rice Blast with High Genome Collinearity
title_sort molecular breeding of different ecotype i japonica i varieties resistant to rice blast with high genome collinearity
topic <i>Japonica</i> rice
blast resistance
yield
quality
<i>Pigm</i>
<i>Hd1</i>
url https://www.mdpi.com/2223-7747/14/12/1836
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