Effect of leptin on porcine adipocytes mRNA expression of lipid droplet related protein and adipogenetic related enzyme
To investigate the effect of leptin on porcine adipocytes mRNA expression of lipid droplet related protein (perilipin, ADRP, TIP47) and adipogenetic related enzyme (FAS, SCD α, ACC α), the adipocytes were treated for 3, 6 h with 0, 50, 100, 150 nmol·L<sup>-1</sup> leptin, and then identi...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.05.006 |
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| Summary: | To investigate the effect of leptin on porcine adipocytes mRNA expression of lipid droplet related protein (perilipin, ADRP, TIP47) and adipogenetic related enzyme (FAS, SCD α, ACC α), the adipocytes were treated for 3, 6 h with 0, 50, 100, 150 nmol·L<sup>-1</sup> leptin, and then identified with red oil staining and tested by semiquantitative RT-PCR. The result shows that, when the adipocytes was treated with 150 nmol·L<sup>-1</sup> leptin for 3 h, the expression of perilipin mRNA was decreased remarkably; treated with 50 and 100 nmol·L<sup>-1</sup> leptin for 3 h, the expression of PPAR γ, ACC α, FAS mRNA were decreased (P<0.05); when the adipocytes was treated with 50, 100 nmol·L<sup>-1</sup> leptin for 6 h, the expression of perilipin, SCD α, PPARγ, FAS, ACC α, LXR α mRNA were decreased; treated with 50, 100, 150 nmol·L<sup>-1</sup> leptin for 6 h, the expression of ADRP mRNA was decreased; treated with 100, 150 nmol·L<sup>-1</sup> leptin for 6 h, the expression of TIP47 mRNA was increased. The findings above suggested that 50, 100 nmol·L<sup>-1</sup> leptin may inhibit the expression of perilipin, SCD α, FAS, ACC α mRNA through inhibiting PPAR γ and LXR α to regulate lipid metabolism in the porcine adipocytes, and 150 nmol·L<sup>-1</sup> leptin may cause leptin resistance, and then reduced the effect of leptin. |
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| ISSN: | 1008-9209 2097-5155 |