Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women

Adipose tissue receptors C5aR and C5L2 and their heterodimerization/functionality and interaction with ligands C5a and acylation stimulating protein (ASP) have been evaluated in cell and rodent studies. Their contribution to obesity factors in humans remains unclear. We hypothesized that C5a recepto...

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Main Authors: Pegah Poursharifi, Reza Rezvani, Abhishek Gupta, Marc Lapointe, Picard Marceau, André Tchernof, Katherine Cianflone
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2014/413921
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author Pegah Poursharifi
Reza Rezvani
Abhishek Gupta
Marc Lapointe
Picard Marceau
André Tchernof
Katherine Cianflone
author_facet Pegah Poursharifi
Reza Rezvani
Abhishek Gupta
Marc Lapointe
Picard Marceau
André Tchernof
Katherine Cianflone
author_sort Pegah Poursharifi
collection DOAJ
description Adipose tissue receptors C5aR and C5L2 and their heterodimerization/functionality and interaction with ligands C5a and acylation stimulating protein (ASP) have been evaluated in cell and rodent studies. Their contribution to obesity factors in humans remains unclear. We hypothesized that C5a receptors, classically required for host defense, are also associated with adiposity. Anthropometry and fasting blood parameters were measured in 136 women divided by body mass index (BMI): normal/overweight (≤30 kg/m2; n = 34), obese I (≤45 kg/m2; n = 33), obese II (≤51 kg/m2; n = 33), and obese III (≤80 kg/m2; n = 36). Subcutaneous and omental adipose tissue C5aR and C5L2 expression were analysed. C5L2 expression was comparable between subcutaneous and omental across all BMI groups. Plasma ASP and ASP/omental C5L2 expression increased with BMI (P < 0.001 and P < 0.01, resp.). While plasma C5a was unchanged, C5aR expression decreased with increasing BMI in subcutaneous and omental tissues (P < 0.01 and P < 0.05, resp.), with subcutaneous omental depots. Omental C5L2/C5aR ratio increased with BMI (P < 0.01) with correlations between C5L2/C5aR and waist circumference, HDL-C, and adiponectin. Tissue and BMI differences in receptors and ligands, particularly in omental, suggest relationship to metabolic disturbances and highlight adipose-immune interactions.
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spelling doaj-art-329798c611de408aab756d08467ca0342025-08-20T02:24:18ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/413921413921Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in WomenPegah Poursharifi0Reza Rezvani1Abhishek Gupta2Marc Lapointe3Picard Marceau4André Tchernof5Katherine Cianflone6Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaCentre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec (CRIUCPQ), Laval University, Y4323, 2725 Chemin Ste-Foy, Québec, QC, G1V 4G5, CanadaAdipose tissue receptors C5aR and C5L2 and their heterodimerization/functionality and interaction with ligands C5a and acylation stimulating protein (ASP) have been evaluated in cell and rodent studies. Their contribution to obesity factors in humans remains unclear. We hypothesized that C5a receptors, classically required for host defense, are also associated with adiposity. Anthropometry and fasting blood parameters were measured in 136 women divided by body mass index (BMI): normal/overweight (≤30 kg/m2; n = 34), obese I (≤45 kg/m2; n = 33), obese II (≤51 kg/m2; n = 33), and obese III (≤80 kg/m2; n = 36). Subcutaneous and omental adipose tissue C5aR and C5L2 expression were analysed. C5L2 expression was comparable between subcutaneous and omental across all BMI groups. Plasma ASP and ASP/omental C5L2 expression increased with BMI (P < 0.001 and P < 0.01, resp.). While plasma C5a was unchanged, C5aR expression decreased with increasing BMI in subcutaneous and omental tissues (P < 0.01 and P < 0.05, resp.), with subcutaneous omental depots. Omental C5L2/C5aR ratio increased with BMI (P < 0.01) with correlations between C5L2/C5aR and waist circumference, HDL-C, and adiponectin. Tissue and BMI differences in receptors and ligands, particularly in omental, suggest relationship to metabolic disturbances and highlight adipose-immune interactions.http://dx.doi.org/10.1155/2014/413921
spellingShingle Pegah Poursharifi
Reza Rezvani
Abhishek Gupta
Marc Lapointe
Picard Marceau
André Tchernof
Katherine Cianflone
Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women
Mediators of Inflammation
title Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women
title_full Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women
title_fullStr Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women
title_full_unstemmed Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women
title_short Association of Immune and Metabolic Receptors C5aR and C5L2 with Adiposity in Women
title_sort association of immune and metabolic receptors c5ar and c5l2 with adiposity in women
url http://dx.doi.org/10.1155/2014/413921
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