Human papillomavirus in canine serum: evidence from a Chinese study

IntroductionHuman papillomaviruses (HPVs) are well-known for causing both benign and malignant epithelial growths in humans, but their occurrence in non-human species is rarely reported. Expanding the understanding of HPV’s host range is essential for assessing its ecological and public health impli...

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Main Authors: Yumeng Liu, Lulu Xie, Yimin Zhou, Lin Zhou, Jingshan Bi, Min Zheng, Tian Lan, Wenchao Sun
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Veterinary Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2025.1511289/full
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author Yumeng Liu
Yumeng Liu
Lulu Xie
Yimin Zhou
Lin Zhou
Jingshan Bi
Min Zheng
Tian Lan
Wenchao Sun
author_facet Yumeng Liu
Yumeng Liu
Lulu Xie
Yimin Zhou
Lin Zhou
Jingshan Bi
Min Zheng
Tian Lan
Wenchao Sun
author_sort Yumeng Liu
collection DOAJ
description IntroductionHuman papillomaviruses (HPVs) are well-known for causing both benign and malignant epithelial growths in humans, but their occurrence in non-human species is rarely reported. Expanding the understanding of HPV’s host range is essential for assessing its ecological and public health implications.MethodsWe investigated serum samples from dogs collected in Guangxi, China, between 2014 and 2020. PCR screening was performed to detect HPV DNA, followed by sequencing and phylogenetic analysis of the positive amplicons.ResultsHPV DNA was unexpectedly detected in 2 out of 1,226 canine serum samples, yielding a detection rate of 0.16%. Phylogenetic analysis revealed that the sequences clustered with alpha2-HPV78 (GX-70-related) and alpha2-HPV94 (GX-47-related), respectively. Interestingly, both sequences displayed multiple amino acid variations in viral proteins. However, virus isolation was not achieved.DiscussionThe detection of HPV nucleic acids in canine serum suggests a potential presence of HPV in canine hosts. These findings provide new insights into the possible host range of HPV, underscoring the need for further research to assess the virus’s infectivity, transmission dynamics, and implications for both animal and human health.
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spelling doaj-art-3292d678481a47a4abedcc764fff7fbf2025-08-20T02:19:15ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-04-011210.3389/fvets.2025.15112891511289Human papillomavirus in canine serum: evidence from a Chinese studyYumeng Liu0Yumeng Liu1Lulu Xie2Yimin Zhou3Lin Zhou4Jingshan Bi5Min Zheng6Tian Lan7Wenchao Sun8Institute of Virology, Wenzhou University, Wenzhou, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaInstitute of Virology, Wenzhou University, Wenzhou, ChinaInstitute of Virology, Wenzhou University, Wenzhou, ChinaInstitute of Virology, Wenzhou University, Wenzhou, ChinaGuangxi Centre for Animal Disease Control and Prevention, Nanning, ChinaGuangxi Centre for Animal Disease Control and Prevention, Nanning, ChinaInstitute of Virology, Wenzhou University, Wenzhou, ChinaInstitute of Virology, Wenzhou University, Wenzhou, ChinaIntroductionHuman papillomaviruses (HPVs) are well-known for causing both benign and malignant epithelial growths in humans, but their occurrence in non-human species is rarely reported. Expanding the understanding of HPV’s host range is essential for assessing its ecological and public health implications.MethodsWe investigated serum samples from dogs collected in Guangxi, China, between 2014 and 2020. PCR screening was performed to detect HPV DNA, followed by sequencing and phylogenetic analysis of the positive amplicons.ResultsHPV DNA was unexpectedly detected in 2 out of 1,226 canine serum samples, yielding a detection rate of 0.16%. Phylogenetic analysis revealed that the sequences clustered with alpha2-HPV78 (GX-70-related) and alpha2-HPV94 (GX-47-related), respectively. Interestingly, both sequences displayed multiple amino acid variations in viral proteins. However, virus isolation was not achieved.DiscussionThe detection of HPV nucleic acids in canine serum suggests a potential presence of HPV in canine hosts. These findings provide new insights into the possible host range of HPV, underscoring the need for further research to assess the virus’s infectivity, transmission dynamics, and implications for both animal and human health.https://www.frontiersin.org/articles/10.3389/fvets.2025.1511289/fullpapillomavirushuman papillomaviruscanine serumphylogenetic analysispublic health
spellingShingle Yumeng Liu
Yumeng Liu
Lulu Xie
Yimin Zhou
Lin Zhou
Jingshan Bi
Min Zheng
Tian Lan
Wenchao Sun
Human papillomavirus in canine serum: evidence from a Chinese study
Frontiers in Veterinary Science
papillomavirus
human papillomavirus
canine serum
phylogenetic analysis
public health
title Human papillomavirus in canine serum: evidence from a Chinese study
title_full Human papillomavirus in canine serum: evidence from a Chinese study
title_fullStr Human papillomavirus in canine serum: evidence from a Chinese study
title_full_unstemmed Human papillomavirus in canine serum: evidence from a Chinese study
title_short Human papillomavirus in canine serum: evidence from a Chinese study
title_sort human papillomavirus in canine serum evidence from a chinese study
topic papillomavirus
human papillomavirus
canine serum
phylogenetic analysis
public health
url https://www.frontiersin.org/articles/10.3389/fvets.2025.1511289/full
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