Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere
Abstract High-throughput sequencing of the 16 S rRNA gene of bacteria and the 18 S rRNA gene of fungi was employed to characterize the compositional diversity of the rhizosphere microbial community in cucumbers (Cucumis sativus L.), bitter gourds (Momordica charantia L.), and eggplants (Solanum melo...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-11657-9 |
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| author | Yuling Yin Yuan Liu Shaochun Luo Jinsong Zhou |
| author_facet | Yuling Yin Yuan Liu Shaochun Luo Jinsong Zhou |
| author_sort | Yuling Yin |
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| description | Abstract High-throughput sequencing of the 16 S rRNA gene of bacteria and the 18 S rRNA gene of fungi was employed to characterize the compositional diversity of the rhizosphere microbial community in cucumbers (Cucumis sativus L.), bitter gourds (Momordica charantia L.), and eggplants (Solanum melongena L.) with or without asparagus straw return. Asparagus straw return caused significant changes in the bacterial and fungal community composition and abundance of the three vegetables. The dominant bacterial phyla in all the treatments were primarily Actinobacteria, Firmicutes, Gemmatimonadetes, Bacteroidetes, Chloroflexi, Acidobacteria, and Proteobacteria. The dominant fungal phyla were Ciliophora, Ascomycota, and Basidiomycota. The relative abundance of the bacterial phylum Actinobacteria was greater in HG1, KG1, and QZ1 and decreased by approximately 20% with asparagus straw return, whereas that of Firmicutes was greater in the two treatments to which asparagus straw was added to all three vegetable soils, which were nearly 20–30 times greater than those in HG1, KG1, and QZ1. Asparagus straw return was a crucial factor in the formation of clusters according to the dominant OTUs. Asparagus straw return increased the bacterial diversity in the rhizosphere of eggplants. Special beneficial functional microbes, such as some Paenibacillus and Cephaliophora_tropica for cucumbers and Lysinibacillus and Ramlibacter for eggplants, are affected by certain vegetables. Moreover, the vigour of eggplants treated with asparagus straw return was greater than that of the other vegetables. The fresh weights increased at straw rates of 15 g/kg and 25 g/kg in eggplants (QZ2 and QZ3), with the greatest percentage reaching approximately 200%. Thus, eggplants might be considered a better option for planting after asparagus straw application in the field. Straw return was the main factor affecting rhizosphere microorganisms but it also affected plant species in the soil microbial community, which could allow us to understand the use of straw return. |
| format | Article |
| id | doaj-art-328d3d6889f94510b0476dffda7b8589 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-328d3d6889f94510b0476dffda7b85892025-08-20T03:04:25ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-11657-9Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphereYuling Yin0Yuan Liu1Shaochun Luo2Jinsong Zhou3Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural SciencesLiaoning Saline or Alkaline Land Utilization and Research Institute, Liaoning Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Jiangxi Academy of Agricultural SciencesInstitute of Vegetables and Flowers, Jiangxi Academy of Agricultural SciencesAbstract High-throughput sequencing of the 16 S rRNA gene of bacteria and the 18 S rRNA gene of fungi was employed to characterize the compositional diversity of the rhizosphere microbial community in cucumbers (Cucumis sativus L.), bitter gourds (Momordica charantia L.), and eggplants (Solanum melongena L.) with or without asparagus straw return. Asparagus straw return caused significant changes in the bacterial and fungal community composition and abundance of the three vegetables. The dominant bacterial phyla in all the treatments were primarily Actinobacteria, Firmicutes, Gemmatimonadetes, Bacteroidetes, Chloroflexi, Acidobacteria, and Proteobacteria. The dominant fungal phyla were Ciliophora, Ascomycota, and Basidiomycota. The relative abundance of the bacterial phylum Actinobacteria was greater in HG1, KG1, and QZ1 and decreased by approximately 20% with asparagus straw return, whereas that of Firmicutes was greater in the two treatments to which asparagus straw was added to all three vegetable soils, which were nearly 20–30 times greater than those in HG1, KG1, and QZ1. Asparagus straw return was a crucial factor in the formation of clusters according to the dominant OTUs. Asparagus straw return increased the bacterial diversity in the rhizosphere of eggplants. Special beneficial functional microbes, such as some Paenibacillus and Cephaliophora_tropica for cucumbers and Lysinibacillus and Ramlibacter for eggplants, are affected by certain vegetables. Moreover, the vigour of eggplants treated with asparagus straw return was greater than that of the other vegetables. The fresh weights increased at straw rates of 15 g/kg and 25 g/kg in eggplants (QZ2 and QZ3), with the greatest percentage reaching approximately 200%. Thus, eggplants might be considered a better option for planting after asparagus straw application in the field. Straw return was the main factor affecting rhizosphere microorganisms but it also affected plant species in the soil microbial community, which could allow us to understand the use of straw return.https://doi.org/10.1038/s41598-025-11657-9AsparagusStraw returnMicrobial community diversityVegetables |
| spellingShingle | Yuling Yin Yuan Liu Shaochun Luo Jinsong Zhou Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere Scientific Reports Asparagus Straw return Microbial community diversity Vegetables |
| title | Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere |
| title_full | Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere |
| title_fullStr | Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere |
| title_full_unstemmed | Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere |
| title_short | Influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere |
| title_sort | influence of asparagus straw returns associated with vegetable species on microbial diversity in the rhizosphere |
| topic | Asparagus Straw return Microbial community diversity Vegetables |
| url | https://doi.org/10.1038/s41598-025-11657-9 |
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