A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture
Acclimatization is the final stage of propagation by in vitro culture. This phase is crucial in micropropagation, as it will determine the survival of plantlets outside culture jars. This phase’s efforts should ensure optimal conditions, including the media type. This study sought the best media for...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM Universitas Sebelas Maret
2024-06-01
|
| Series: | AgriHealth |
| Subjects: | |
| Online Access: | https://jurnal.uns.ac.id/agrihealth/article/view/85776 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850138038388129792 |
|---|---|
| author | Rindang Dwiyani Yuyun Fitriani Francis Geren Sta Ana Putu Oki Bimantara |
| author_facet | Rindang Dwiyani Yuyun Fitriani Francis Geren Sta Ana Putu Oki Bimantara |
| author_sort | Rindang Dwiyani |
| collection | DOAJ |
| description | Acclimatization is the final stage of propagation by in vitro culture. This phase is crucial in micropropagation, as it will determine the survival of plantlets outside culture jars. This phase’s efforts should ensure optimal conditions, including the media type. This study sought the best media for acclimating strawberry plantlets produced under meristem culture. The research employed the randomized block design, utilizing 7 various media treatments, which include husk charcoal; perlite; cocopeat; perlite + husk charcoal; soil + sand; husk charcoal + cocopeat; and perlite + cocopeat. The media mix was set at 1:1 a ratio (weight/weight), with 9 replicates. The chemical properties of the media were then analyzed, including several parameters such as organic C, total N, and water content. Several parameters were observed for plantlet’s growth parameters, including plant survival rates, number of leaves per plant, average leaf area, and root fresh weight. The results found that cocopeat was the best medium for acclimating strawberry plantlets. In cocopeat media, the plant survival rate reached 96.68%, with the leaves number of 7.67 plant-1, an average leaf area of 120.92 cm2 plant-1, and root fresh weight of 4.30 g plant-1. These results indicate that cocopeat is a medium derived from coconut fiber powder, a natural resource that can be renewed sustainably and produces better plant plantlet growth. |
| format | Article |
| id | doaj-art-32839bc8fba2414cb74f55ad0684a3a6 |
| institution | OA Journals |
| issn | 2722-0656 2722-0648 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Research and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM Universitas Sebelas Maret |
| record_format | Article |
| series | AgriHealth |
| spelling | doaj-art-32839bc8fba2414cb74f55ad0684a3a62025-08-20T02:30:39ZengResearch and Development Center for Food, Nutrition and Public Health (P4GKM) LPPM Universitas Sebelas MaretAgriHealth2722-06562722-06482024-06-0152859110.20961/agrihealth.v5i2.8577642261A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem CultureRindang Dwiyani0Yuyun Fitriani1Francis Geren Sta Ana2Putu Oki Bimantara3Department of Agroecotechnology, Faculty of Agriculture, Universitas Udayana, BaliDepartment of Agroecotechnology, Faculty of Agriculture, Universitas Udayana, BaliDepartment of Environmental Science, Thai Nguyen University of Agriculture and Forestry, Thai NguyenDepartment of Agroecotechnology, Faculty of Agriculture, Universitas Udayana, BaliAcclimatization is the final stage of propagation by in vitro culture. This phase is crucial in micropropagation, as it will determine the survival of plantlets outside culture jars. This phase’s efforts should ensure optimal conditions, including the media type. This study sought the best media for acclimating strawberry plantlets produced under meristem culture. The research employed the randomized block design, utilizing 7 various media treatments, which include husk charcoal; perlite; cocopeat; perlite + husk charcoal; soil + sand; husk charcoal + cocopeat; and perlite + cocopeat. The media mix was set at 1:1 a ratio (weight/weight), with 9 replicates. The chemical properties of the media were then analyzed, including several parameters such as organic C, total N, and water content. Several parameters were observed for plantlet’s growth parameters, including plant survival rates, number of leaves per plant, average leaf area, and root fresh weight. The results found that cocopeat was the best medium for acclimating strawberry plantlets. In cocopeat media, the plant survival rate reached 96.68%, with the leaves number of 7.67 plant-1, an average leaf area of 120.92 cm2 plant-1, and root fresh weight of 4.30 g plant-1. These results indicate that cocopeat is a medium derived from coconut fiber powder, a natural resource that can be renewed sustainably and produces better plant plantlet growth.https://jurnal.uns.ac.id/agrihealth/article/view/85776acclimatizationcocopeatmeristem culturerootingsurvival rate |
| spellingShingle | Rindang Dwiyani Yuyun Fitriani Francis Geren Sta Ana Putu Oki Bimantara A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture AgriHealth acclimatization cocopeat meristem culture rooting survival rate |
| title | A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture |
| title_full | A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture |
| title_fullStr | A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture |
| title_full_unstemmed | A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture |
| title_short | A Study of Acclimatization Media on Strawberry (Fragaria x ananassa Duch.) Plantlets Produced from Meristem Culture |
| title_sort | study of acclimatization media on strawberry fragaria x ananassa duch plantlets produced from meristem culture |
| topic | acclimatization cocopeat meristem culture rooting survival rate |
| url | https://jurnal.uns.ac.id/agrihealth/article/view/85776 |
| work_keys_str_mv | AT rindangdwiyani astudyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT yuyunfitriani astudyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT francisgerenstaana astudyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT putuokibimantara astudyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT rindangdwiyani studyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT yuyunfitriani studyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT francisgerenstaana studyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture AT putuokibimantara studyofacclimatizationmediaonstrawberryfragariaxananassaduchplantletsproducedfrommeristemculture |